Clinical significance and outcome of Aeromonas infection among 19 patients-a descriptive study from south India

Vithiya, G.; Raja, S

doi:10.1016/j.ijmmb.2022.03.001

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…The infections caused by Aeromonas can involve more than one strain or strains belonging to Aeromonas or other genera ( Lamy et al, 2009 ; Chao et al, 2013 ; Barraud et al, 2020 ; Lin et al, 2020 ; Vithiya and Raja, 2022 ). However, to the best of our knowledge, very few studies have investigated the potential interactions of the isolated bacteria.…”

Section: Discussionmentioning

confidence: 99%

Interactions between Aeromonas caviae and Yersinia enterocolitica isolated from a case of diarrhea: evaluation of antimicrobial susceptibility and immune response of infected macrophages

Fernández-Bravo,

Recio,

Figueras

2024

Front. Microbiol.

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Aeromonas species cause a wide spectrum of human diseases, primarily gastroenteritis, septicemia, and wound infections. Several studies have shown that about 40% of these cases involve mixed or polymicrobial infections between Aeromonas spp. and bacteria from other genera. However, the immune response of macrophages in front of the bacteria present in the mixed infections, as well as their impact on antimicrobial therapy, have not been investigated. This study evaluated the cell damage and immune response of the mouse macrophage BALB/c cell line (J774A.1) after performing a single and a mixed infection with a strain of Aeromonas caviae and Yersinia enterocolitica, both recovered from the same fecal sample from a patient with diarrhea. Macrophage cell damage was measured by the release of lactate dehydrogenase (LDH) while the immune response was evaluated studying the expression by RT-qPCR of six relevant immune-related genes. Additionally, the antimicrobial susceptibility pattern of the single and mixed strains in front of seventeen antibiotics was evaluated to determine the potential impact on the infection treatment. Macrophages infected with the mixture of the two strains showed a higher cell damage in comparison with the single infections and the immune-related genes, i.e., cytokines and chemokines genes (TNF-α, CCL20), and apoptotic and pyroptotic genes (TP53 and IL-1β) were overexpressed. After infection with the mixed cultures, an increase in the antimicrobial resistance was observed for ciprofloxacin, trimethoprim, chloramphenicol, gentamicin and ertapenem. This study increased the knowledge about the synergetic effect of the bacteria involved in mixed infection and on their potential impact on the treatment and evolution of the infection.

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