Clinical significance of "anti-HBc alone" in human immunodeficiency virus-positive patients

Pérez-Rodríguez, M. Teresa; Sopeña, B.; Crespo, Manuel; Rivera, Alberto; Blanco, Teresa González del; Ocampo, Antonio; Martínez-Vázquez, C.

doi:10.3748/wjg.15.1237

Cited by 29 publications

(24 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In some individuals, the only serological marker of HBV infection is the presence of anti-HBc antibodies (10,24), and thus detection of "anti-HBcAg alone" could reflect unrecognized "occult" HBV infection and physicians should consider investigating such patients with HBV molecular tests (21). Additionally, isolated anti-HBc can be used as a marker to assess the risk of HBV reactivation in patients undergoing therapy that could result in immunosuppression or patients who are HIV positive (17) or hepatitis C virus (HCV) positive (25).…”

mentioning

confidence: 99%

Novel Double-Antigen Sandwich Immunoassay for Human Hepatitis B Core Antibody

Yuan

Huang

et al. 2010

Clin Vaccine Immunol

View full text Add to dashboard Cite

A novel diagnostic immunoassay testing procedure for hepatitis B virus core antibody (anti-HBc) using homogeneous purified full-length hepatitis B virus core antigen (HBcAg) capsids obtained from Escherichia coli was compared with Abbott Architect anti-HBc chemiluminescent microparticle immunoassay (CMIA; indirect method) against a library of specimens. A monoclonal anti-HBc neutralization confirmatory assay was then used to determine the degree of discordance between specimens. The new assay was found to be superior in both sensitivity and specificity.Antibodies (anti-HBc) to hepatitis B virus (HBV) core antigen (HBcAg) are present in current and past HBV infections. Anti-HBc antibodies represent a long-term serological marker of HBV infection, initially appearing during the acute phase of the infection and generally persisting thereafter (16). Thus, as anti-HBc is a universal marker of HBV infection, routine blood donor screening for anti-HBc has been implemented in some countries with low endemicity. It is a cost-effective method of screening. Such screening procedures have resulted in a decrease in the risk of posttransfusion HBV infections (12). In some individuals, the only serological marker of HBV infection is the presence of anti-HBc antibodies (10, 24), and thus detection of "anti-HBcAg alone" could reflect unrecognized "occult" HBV infection and physicians should consider investigating such patients with HBV molecular tests (21). Additionally, isolated anti-HBc can be used as a marker to assess the risk of HBV reactivation in patients undergoing therapy that could result in immunosuppression or patients who are HIV positive (17) or hepatitis C virus (HCV) positive (25).With these uses in mind, having a more efficient and reliable assay for anti-HBc is desirable. Most current commercially available anti-HBc assays have poor sensitivity or specificity (2, 19) and can be attributed to the inferior performance of the competitive immunoassay, especially for detecting low-titer antiHBc-reactive samples. False-positive reactivity can partially be attributed to unspecific activation of premature B lymphocytes causing the production of IgM, IgA, or IgM-related molecules without previous exposure to HBV (18,19). The specificity of competitive assays for anti-HBc can be significantly improved by addition of mild reducing agents, but such modified procedures often lead to the loss of sensitivity, particularly for IgM anti-HBc (23). In this study, a novel immunoassay for anti-HBc based on the double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) method is compared with a commercial anti-HBc assay, the Architect chemiluminescent microparticle immunoassay (CMIA). MATERIALS AND METHODSExpression and purification of full-length rHBcAg in E. coli. Two full-length HBcAg genes were obtained by chemosynthesis: AB090269 (genotype D, named CpD) and AB014368 (genotype C, named CpC). The rare arginine codes in Escherichia coli (AGA and AGG) located in the HBcAg gene were changed to CGT. The two fragments were inse...

show abstract

mentioning

confidence: 99%

Novel Double-Antigen Sandwich Immunoassay for Human Hepatitis B Core Antibody

Yuan

Huang

et al. 2010

Clin Vaccine Immunol

View full text Add to dashboard Cite

show abstract

“…Careful follow-up is required in order to start, complete and booster vaccination schedules in HIV patients, not only for HBV but probably also for other vaccine antigens. For all these reasons, the doctor who is informing you, an expert in the management of pediatric HIV infection, considers it appropriate for you to take part in the Implementation of Occult Hepatitis Screening in the 152 Spanish Cohort of HIV-Infected Pediatric Patients study that is going to be carried out in several Spanish pediatric hospitals.…”

Section: Discussionmentioning

confidence: 99%

“…However, the results are not conclusive since liver biopsy for HBV DNA intrahepatic detection was not performed, and children participating in the study showed a high median age and good clinical and immunological status, as good as those in similar studies. Thus, the interpretation of the HBV marker profile in the 6 patients with probable OBI in the present study remains controversial in terms of its being a surrogate marker for HBV/HIV coinfection, or possibly not in the event of additional immunosuppresion (144,152,157,160). …”

mentioning

confidence: 81%

See 1 more Smart Citation

Implementation of Occult Hepatitis Screening in the Spanish Cohort of HIV-infected Pediatric Patients

Dapena

Figueras

Noguera-Julián³

et al. 2013

Pediatric Infectious Disease Journal

View full text Add to dashboard Cite

“…A presença de anti-HBc isolado é frequente, ocorrendo em cerca de 30% na população coinfectada, com relevância clínica indeterminada [96][97][98] .…”

Section: Portadores Inativos Dounclassified

Evolução dos marcadores sorológicos da hepatite B, AgHBs e AgHBe, em pacientes AgHBs positivos coinfectados com o vírus da imunodeficiência humana (HIV)

Toscano¹

View full text Add to dashboard Cite

Clinical significance of "anti-HBc alone" in human immunodeficiency virus-positive patients

Cited by 29 publications

References 23 publications

Novel Double-Antigen Sandwich Immunoassay for Human Hepatitis B Core Antibody

Novel Double-Antigen Sandwich Immunoassay for Human Hepatitis B Core Antibody

Implementation of Occult Hepatitis Screening in the Spanish Cohort of HIV-infected Pediatric Patients

Evolução dos marcadores sorológicos da hepatite B, AgHBs e AgHBe, em pacientes AgHBs positivos coinfectados com o vírus da imunodeficiência humana (HIV)

Contact Info

Product

Resources

About