Clinical significance of expression of apoptotic signal proteins in gastric carcinoma tissue

Abstract

Abstract Abstract Abstract AIM:To evaluate the expressions of apoptotic signal proteins FADD, TRADD, FasL, Fas, and NFκB in gastric carcinoma tissues and their clinical significance. METHODS:Western blot immune trace method was adopted to detect the expressions of apoptotic signal proteins FADD, TRADD, FasL, Fas, and NFκB in 55 tissue specimens of gastric carcinoma. RESULTS:Five apoptotic signal proteins had different expressions in the gastric carcinoma samples and their expressions were not correlated to age (P = 0.085). Expressions of the FADD, FasL, Fas, and NFκB proteins reduced with increase of the volume of tumor with the exception of increased expression the TRADD protein (64.7-71.1%, P = 0.031). With gradual increase of the malignancy of gastric carcinoma tissues, expressions of the FADD, FasL, and Fas proteins decreased (78.6-28.0%, P = 0.008; 78.6-65.9%, P = 0.071; 100.0-46.3%, P = 0.014), while expressions of the TRADD and NFκB proteins increased (42.9-78.1%, P = 0.063; 78.6-79.1%, P = 0.134). With gradual increase of serum CEA, expression of the FADD protein decreased (62.5-34.0%, P = 0.073), but expressions of the TRADD, FasL, Fas, and NFκB proteins increased (0.0-80.8%, P = 0.005; 62.5-70.2%, P = 0.093; 0.0-70.2%, P = 0.003; 62.5-80.9%, P = 0.075). When compared to the tissues of gastric carcinoma without metastasis, the positive rate of expressions of the FADD and FasL proteins increased, whereas expressions of the TRADD, FADD, and NFκB proteins decreased. There was no significant difference between them (P = 0.095). CONCLUSION:Gastric carcinoma is endurable to Fasrelated apoptosis and apoptotic signal proteins are differently expressed in gastric carcinoma.

miR‐106a is frequently upregulated in gastric cancer and inhibits the extrinsic apoptotic pathway by targeting FAS

“…Moreover, in order to study the relationship between miR‐106a and FAS in vivo, FAS expression was examined by immunohistochemical staining in tissue specimens. We found that the expression of FAS was much lower in gastric carcinoma tissue samples than in normal gastric mucosa, which has also been found by other groups [27–29]. Even though a clear reverse correlation between FAS and miR‐106a in tissue specimens was found, the correlation was weak ( r = −0.409).…”

“…As miR‐106a was frequently upregulated in gastric cancer and was predicted to target FAS to inhibit cell apoptosis, we used immunohistochemical staining to determine whether FAS expression was associated with stomach cancer (Figure 6E). The results showed that FAS protein level was significantly decreased in gastric cancer samples compared with their nontumor counterparts (15.4% vs. 84.6%, Z = −4.944, P < 0.001), as reported by other groups [27–29]. To assess the clinical relevance of these findings, we correlated FAS level with miR‐106a expression in the same patients.…”

Expression of nuclear factor κB in human gastric carcinoma: relationship with IκBa and prognostic significance

“…A wide spectrum of molecular markers, especially those involved in the regulation of cell apoptosis, has been tested as potentially useful parameters to predict clinical outcome in gastric cancer [32,42]. These include interleukin 1β, interleukin 8, tumour necrosis factor alpha [21] and COX-2 [22].…”