Clinical Significance of Folate Receptor-positive Circulating Tumor Cells Detected by Ligand-targeted Polymerase Chain Reaction in Lung Cancer

Wang, Lin; Wu, Chuanyong; Qiao, Lihua; Yu, Wenjing; Guo, Qiaojuan; Zhao, Mingna; Yang, Guohua; Zhao, Hang; Lou, Jiatao

doi:10.7150/jca.16856

Cited by 54 publications

(57 citation statements)

References 30 publications

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“…ABI7300 instruments (Life Technologies, Carlsbad, CA, USA) were used to collect PCR signals and data. The reaction conditions were: 95°C, 2 minutes; 40°C, 30 seconds; 72°C, 30 seconds; 8°C, 5 minutes; 40 cycles, 95°C, 10 seconds; 35°C, 30 seconds; and 72°C, 10 seconds …”

Section: Methodsmentioning

confidence: 99%

Circulating tumor cell levels and carcinoembryonic antigen: An improved diagnostic method for lung adenocarcinoma

Ding

Zhou

et al. 2018

Thoracic Cancer

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BackgroundThe aim of this study was to determine a correlation between benign and malignant lung solitary pulmonary nodules (SPN), and analyze the association between circulating tumor cell (CTC) levels and different subtypes of lung adenocarcinoma.MethodsA total of 200 patients (80 with SPNs and 120 diagnosed with lung cancer) were included in the study. The CTC levels were quantified by identifying the folate receptor on the surface of tumor cells; clinical tumor specific markers were detected by biochemical immunization. The content of peripheral blood CTCs in benign and malignant lung SPN patients was detected and the differences in preoperative CTC levels in different pathological subtypes were analyzed. Based on the collected data, receiver operating characteristic curves were calculated and the rate of lung cancer was predicted.ResultsThe peripheral blood CTC levels in patients with malignant lung SPNs were higher than in patients with benign SPNs. The maximum nodule diameter, carcinoembryonic antigen, and CTC levels were independent risk factors for malignant lung SPNs. The peripheral blood CTC levels in patients with stage III–IV lung adenocarcinoma were higher than in stage I–II patients. The peripheral blood CTC levels in patients with microinvasive and invasive adenocarcinoma were higher than in adenocarcinoma in situ patients. The CTC levels in the peripheral blood of patients with maximum tumor diameter > 2 cm were higher than in patients with tumors < 2 cm.ConclusionThe detection of CTCs can be used as a biomarker for screening SPNs and diagnosing early‐stage lung cancer. Using the combination of CTC levels and CEA significantly improves the efficacy of lung adenocarcinoma diagnosis.

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Section: Methodsmentioning

confidence: 99%

Circulating tumor cell levels and carcinoembryonic antigen: An improved diagnostic method for lung adenocarcinoma

Ding

Zhou

et al. 2018

Thoracic Cancer

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“…These studies have shown that by using folate receptor as a marker it is possible to discriminate between NSCLC patients, individuals with benign lung disease and healthy subjects. A sensitivity up to 77.7% and a specificity up to 89.5% for the diagnosis of NSCLC was reported (7,9). However, it must be emphasized that the sensitivity was higher for stage IV patients, while it was <70% in stage I.…”

Section: Ctc As a Diagnostic Marker In Nsclcmentioning

confidence: 99%

“…Different studies have used the folate receptor transcript as a marker to identify the CTC (7)(8)(9). In fact, folate receptor is often expressed in NSCLC cells whereas its levels of expression are usually low in blood cells.…”

Section: Ctc As a Diagnostic Marker In Nsclcmentioning

confidence: 99%

Clinical utility of circulating tumor cells in patients with non-small-cell lung cancer

Gallo

Luca

Maiello

et al. 2017

Transl. Lung Cancer Res.

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Several different studies have addressed the role of the circulating tumor cells (CTC) in nonsmall-cell lung cancer (NSCLC). In particular, the potential of CTC analysis in the early diagnosis of NSCLC and in the prediction of the outcome of patients with early and advanced NSCLC have been explored. A major limit of these studies is that they used different techniques for CTC isolation and enumeration, they employed different thresholds to discriminate between high-and low-risk patients, and they enrolled heterogeneous and often small cohort of patients. Nevertheless, the results of many studies are concordant in indicating a correlation between high CTC count and poor prognosis in both early and advanced NSCLC. The reduction of CTC number following treatment might also represent an important indicator of sensitivity to therapy in patients with metastatic disease. Preliminary data also suggest the potential for CTC analysis in the early diagnosis of NSCLC in high-risk individuals. However, these findings need to be confirmed in large prospective trials in order to be transferred to the clinical practice.The molecular profiling of single CTC in NSCLC might provide important information on tumor biology and on the mechanisms involved in tumor dissemination and in acquired resistance to targeted therapies. In this respect, xenografts derived from CTC might represent a valuable tool to investigate these phenomena and to develop novel therapeutic strategies.

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“…Although CTCs were observed more than 100 years ago, for the first time, their clinical significance has recently recognized. 15 Detection of CTCs might be difficult because of their rare numbers comparing with the number of leukocytes (~1 CTC per 10 5 -10 8 leukocytes) in blood. 13 Although there are many different techniques applied for the detection of CTC, only the CellSearch system which has been approved by the U.S. Food and Drug…”

Section: Discussionmentioning

confidence: 99%

Akciğer kanseri hastalarında dolaşımdaki tümör hücrelerini nasıl tespit ederiz? akım sitometrisi ile yapılan kısa bir çalışma

Guler

Uysal

Simsek

et al. 2018

Online Türk Sağlık Bilimleri Dergisi

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Dolaşımdaki tümör hücreleri (CTC'ler) karsinomların metastatik yayılımında önemli role sahiptir. Bu nedenle, son yıllarda birçok kanser türünde hastalığın seyri ve tedavi etkinliğinin anlaşılmasında yardımcı olabileceği düşünülen CTC'ler üzerine yoğunlaşılmıştır. Teknik yaklaşımlardaki gelişmeler ile özellikle akciğer kanseri gibi dokularına ulaşılması zor olan tümörlerde sıvı biyopsi olarak tüm kandan CTC tespitinin değeri her geçen gün artmaktadır. Tanı anında sıklıkla metastaz yaptıkları teşhis edilen akciğer kanseri, hem kadınlarda hem de erkeklerde kanser ölüm nedenlerinin birincil sebebidir. Çalışmamızın amacı akım sitometrisi ile akciğer kanserli hastaların periferik kan örneklerinde (7.5 mL) CTC tespitidir. Zenginleştirme ve saptama adımlarından oluşan modifiye ettiğimiz yöntemimiz ile CTC sayısının tespit edilmesi için 9 akciğer kanserli birey ve 9 sağlıklı birey çalışmaya dahil edilmiştir. Uyguladığımız metotta zenginleştirme basamağı için fikol yoğunluk gradiyent ayrımı ve immünomanyetik ayırma tekniği (CD45 negatif seçilim) gerçekleştirilmiştir. Sonrasında, zenginleştirilmiş hücreler arasında CTC'leri tespit etmek için, anti-epitelyal hücre adhezyon molekülünün ve sitokeratinlerin ekspresyonuna dayanan çok parametreli akım sitometrisi ile analiz yapılmıştır. Çalışmamız sonucunda akciğer kanserli hastaların tümünde CTC gözlenirken, sağlıklı bireylerde gözlenmemiştir (Z=3.823; p<0.001). Böylece, modifiye ettiğimiz metodun akciğer kanserinde CTC tespitinde kullanılabilirliği gösterilmiştir.

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Clinical Significance of Folate Receptor-positive Circulating Tumor Cells Detected by Ligand-targeted Polymerase Chain Reaction in Lung Cancer

Cited by 54 publications

References 30 publications

Circulating tumor cell levels and carcinoembryonic antigen: An improved diagnostic method for lung adenocarcinoma

Circulating tumor cell levels and carcinoembryonic antigen: An improved diagnostic method for lung adenocarcinoma

Clinical utility of circulating tumor cells in patients with non-small-cell lung cancer

Akciğer kanseri hastalarında dolaşımdaki tümör hücrelerini nasıl tespit ederiz? akım sitometrisi ile yapılan kısa bir çalışma

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