Clinical significance of measurement of plasma annexin V concentration of patients in the emergency room

Matsuda, Ryuko; Kaneko, Noboru; Kikuchi, Masatoshi; Chiwaki, Fumiko; Toda, Masashi; Ieiri, Tamio; Horikawa, Yoshifumi; Shimizu, Mayumi; Shimamoto, Ken

doi:10.1016/s0300-9572(03)00034-0

Cited by 40 publications

(35 citation statements)

References 14 publications

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“…Annexin A2 and A5 are both present in serum (39,40) and can bind negatively charged phospholipids. Additionally, their surface expression on lymphocytes increases during apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Annexin A2 and A5 Serve as New Ligands for C1q on Apoptotic Cells

Martin

Leffler

Blom

2012

Journal of Biological Chemistry

102

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“…Annexin A2 and A5 are both present in serum (39,40) and can bind negatively charged phospholipids. Additionally, their surface expression on lymphocytes increases during apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Annexin A2 and A5 Serve as New Ligands for C1q on Apoptotic Cells

Martin

Leffler

Blom

2012

Journal of Biological Chemistry

102

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“…ANXV belongs to a recently discovered family of proteins, the annexins, is a convenient instrument for the apoptotic cells detection, because in the presence of Ca 2+ this protein preferentially binds to phosphatidylserines and other anionic phospholipids (PLs) [54,55] . ANXV is an important modulator of the immune response against PS-exposing particles like apoptotic cells.…”

Section: Discussionmentioning

confidence: 99%

“…Anionic PLs exposure on the cell surface, due to loss of plasma membrane asymmetry, is an early sign of apoptosis and is observed before apoptosis-specific changes in cell morphology as well as loss of the cell plasma membrane integrity have occurred. The apoptotic cells are cleared via an anti-inflammatory pathway, and the above mentioned sign is a major "eat-me" signal that they send to phagocytes [54,55] . ANXV may interfere in vivo with the immunosuppressive effects of apoptotic cells, since it binds PS with high selectivity and inhibits the uptake of apoptotic cell by phagocytes [54,55] .…”

Section: Discussionmentioning

confidence: 99%

“…The apoptotic cells are cleared via an anti-inflammatory pathway, and the above mentioned sign is a major "eat-me" signal that they send to phagocytes [54,55] . ANXV may interfere in vivo with the immunosuppressive effects of apoptotic cells, since it binds PS with high selectivity and inhibits the uptake of apoptotic cell by phagocytes [54,55] . This protein is expressed by many types of cells and is an in vivo marker of cellular injury and death.…”

Section: Discussionmentioning

confidence: 99%

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Collectins, C3 complement protein, annexin V and C-reactive protein in acute ischemic stroke: interrelation and implication to upregulated apoptosis and inflammation

Boyajyan

Tsakanova

Sim

2014

Inflamm Cell Signal

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Upregulated inflammatory response and apoptosis, both triggered by ischemia, are the most destructive pathologic processes developed after acute ischemic stroke (IS) at both central and peripheral levels. Identification of molecular mediators of these processes and understanding of their pathomechanisms will support the development of therapies, which can significantly improve outcomes of IS-affected subjects.Mannan-binding lectin (MBL), ficolin L (FL), ficolin H (FH) and C3 complement proteins participate in apoptotic cells recognition and clearance through various mechanisms. In the present study we performed comparative determination of the blood levels of MBL, FL and FH, and hemolytic activity of C3 protein (C3H50) as well as apoptosis marker protein, circulating annexin V (cANXV), and inflammatory marker C-reactive protein (CRP) in 99 patients with acute IS on the first day of stroke onset and 110 healthy subjects. Potential correlation between all measured parameters was analyzed. Methods included enzyme-linked immunosorbent, hemolytic, and immunonephelometric assays; statistics were performed by Student's t-test and Pearson's correlation analysis. The obtained data demonstrated significantly increased and correlated with each other blood levels of MBL and C3H50, cANXV and CRP in patients compared to controls. Slight, while significant, changes in the blood levels of FL and FH were also observed. We concluded that post-ischemic response at the first day of IS onset is characterized by sufficiently elevated and positively correlated with each other blood levels of MBL, C3H50, cANXV and CRP as well as by slightly increased blood levels of FH and FL. The obtained results suggest that MBL and C3 protein are implicated in upregulated inflammatory response and apoptosis developed after IS onset at a peripheral level, and that these pathological processes are interrelated and interdependent.

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“…In this respect, it has been reported in humans that plasma levels of annexin V determined by means of ELISA are increased 8-fold in the early phase of acute myocardial infarction, and immediately decrease after the onset of pain [34]. Other circulating markers of apoptosis are currently under investigation.…”

Section: Biochemical Assessmentmentioning

confidence: 99%