Clinical significance of microRNA-183/Ezrin axis in judging the prognosis of patients with osteosarcoma

Mu, Yisila; Zhang, Haoshaqiang; Che, Lixin; Li, Kun

doi:10.1007/s12032-013-0821-3

Cited by 38 publications

(29 citation statements)

References 31 publications

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“…Leonard et al [24] reported that the ezrin gene was highly expressed in human primary osteosarcoma samples and could be considered the new prognostic marker in osteosarcoma. Mu et al [25] proved that ezrin mRNA expression was higher in osteosarcoma than in noncancerous bone tissues, and high ezrin expression was also significantly correlated with the high grade of the tumour. Data presented by Salas et al [26] suggested that ezrin is a useful immunohistochemical marker for differential diagnosis between chondroblastic osteosarcomas and conventional chondrosarcomas, with a specificity of 100 %.…”

Section: Discussionmentioning

confidence: 99%

The clinical significance of changes in ezrin expression in osteosarcoma of children and young adults

Ługowska

Mierzejewska²,

Lenarcik³

et al. 2016

Tumor Biol.

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Ezrin is a protein that functions as a cross-linker between actin cytoskeleton and plasma membrane. Its clinical role in osteosarcoma is unclear. The aim of this study was to investigate, in osteosarcoma, the prognostic value of ezrin expression at biopsy and changes in expression levels after preoperative chemotherapy. Thirty-eight newly diagnosed osteosarcoma patients aged 6-23 years were included. At diagnosis, 20 patients had localized disease, the others had distant metastases. Median follow-up was 75 months (range 13-135). Ezrin expression was assessed immunohistochemically in biopsy tissue and primary tumour specimens resected after chemotherapy. The influence on survival of changes in ezrin expression after chemotherapy was analysed. Ezrin expression was significantly higher after preoperative chemotherapy and changes compared to biopsy tissue were significantly lower in patients with early progression than in patients with relapse or no further evidence of disease (p = 0.006 and p = 0.002, respectively). Similarly, ezrin expression was higher after preoperative chemotherapy and exhibited less change in expression in deceased patients compared to patients surviving more than 5 years (both p = 0.001). Ezrin expression at biopsy was significantly associated with both histopathological aggressiveness (p < 0.001) and tumour size (p = 0.037). The results of this study provide evidence that changes in overexpression of ezrin due to preoperative chemotherapy could be a useful predictive and prognostic marker in patients with osteosarcoma.

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Section: Discussionmentioning

confidence: 99%

The clinical significance of changes in ezrin expression in osteosarcoma of children and young adults

Ługowska

Mierzejewska²,

Lenarcik³

et al. 2016

Tumor Biol.

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show abstract

“…In pediatric cancers, osteosarcoma accounts for 8 % incidence rate and is a leading cause of cancer-related death among young adults [3,4]. The etiology of osteosarcoma involves environment factors and genetic predisposition [5,6].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-144 inhibits the proliferation, apoptosis, invasion, and migration of osteosarcoma cell line F5M2

Cui

Wang

2015

Tumor Biol.

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This study is aimed to investigate the role of microRNA-144 (miR-144) in osteosarcoma cell line F5M2 proliferation, apoptosis, invasion, and metastasis. Between 2007 and 2014, 66 cases of osteosarcoma samples in the corresponding adjacent normal tissue samples were selected from surgical resection or biopsy in the Department of Orthopedics, Shengjing Hospital, China Medical University. MiR-144 levels and Ezrin messenger RNA (mRNA) levels in osteosarcoma and the adjacent bone tissues were detected, and clinical and pathological features were analyzed. Exogenous miR-144 was transfected into human osteosarcoma cell lines at two different concentrations (low and high), and the expression levels of miR-144 and Ezrin protein between highly metastatic osteosarcoma cells and lowly metastatic osteosarcoma cells were compared. Real-time polymerase chain reaction (RT-PCR) and Western blot were used for detecting the expression levels of miR-144 or Ezrin protein, respectively. Cell proliferation was measured by methylthiazol tetrazolium (MTT) assay. Cell invasion and migration was evaluated by Transwell assays. Finally, flow cytometry was employed to determine the cell apoptosis. MiR-144 expression in osteosarcoma tissue was significantly lower than that in the surrounding normal bone tissue (P < 0.001), while Ezrin mRNA expression in osteosarcoma tissue was significantly higher than that in the surrounding normal bone tissue (P < 0.001); correlation analysis showed a significant negative correlation between miR-144 and Ezrin mRNA levels (r = 0.982, P < 0.001). MiR-144 and Ezrin mRNA expressions were significantly related with cell metastasis (P < 0.05) but were not related with other clinical factors such as gender, age, tumor location, tumor size, Enneking staging, and Dahlin's histological classification. The results of RT-PCR showed that the expression level of miR-144 in osteosarcoma cells increased after transfected with exogenous miR-144 mimics, and this increase positively correlated with the transfection concentration of miR-144 mimics. Furthermore, Western blotting revealed a significant and dose-dependent decrease in Ezrin protein levels in F5M2 cells transfected with miR-144. MTT and Transwell assays showed that the invasion and metastasis of F5M2 cells was significantly decreased following exogenous overexpression of miR-144. Our study supports the view that the miR-144 may regulate Ezrin protein expression by inhibiting the invasion and metastasis of osteosarcoma cells.

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“…Moreover, the combined miR-183 downregulation and ezrin upregulation show a positive correlation with high tumor grade, poor response to chemotherapy, positive metastasis and recurrence, and lower five-year survival rate [48]. However, there is no significant association between miR-183/ezrin expression and other clinicopathological parameters, including patients' age, sex, site, histologic type and surgical method [48]. It has been reported that miR-183 mimics markedly diminish the migratory and invasive abilities of F5M2, a high-metastatic human osteosarcoma cell line, by targeting ezrin [49].…”

Section: Mir-183mentioning

confidence: 97%

“…More recently, Mu et al analyzed the correlation of combined miR-183 and ezrin mRNA expression with clinicopathologic features and prognosis in patients with OS. They found that miR-183 expression is significantly decreased but ezrin mRNA expression is increased in specimens derived from OS cases compared with corresponding noncancerous bone tissues [48]. Moreover, the combined miR-183 downregulation and ezrin upregulation show a positive correlation with high tumor grade, poor response to chemotherapy, positive metastasis and recurrence, and lower five-year survival rate [48].…”

Section: Mir-183mentioning

confidence: 99%

“…They found that miR-183 expression is significantly decreased but ezrin mRNA expression is increased in specimens derived from OS cases compared with corresponding noncancerous bone tissues [48]. Moreover, the combined miR-183 downregulation and ezrin upregulation show a positive correlation with high tumor grade, poor response to chemotherapy, positive metastasis and recurrence, and lower five-year survival rate [48]. However, there is no significant association between miR-183/ezrin expression and other clinicopathological parameters, including patients' age, sex, site, histologic type and surgical method [48].…”

Section: Mir-183mentioning

confidence: 99%

See 1 more Smart Citation

MicroRNAs in osteosarcoma

Zhang

Yan

Wang

et al. 2015

Clinica Chimica Acta

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Clinical significance of microRNA-183/Ezrin axis in judging the prognosis of patients with osteosarcoma

Cited by 38 publications

References 31 publications

The clinical significance of changes in ezrin expression in osteosarcoma of children and young adults

The clinical significance of changes in ezrin expression in osteosarcoma of children and young adults

MicroRNA-144 inhibits the proliferation, apoptosis, invasion, and migration of osteosarcoma cell line F5M2

MicroRNAs in osteosarcoma

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