Clinical significance of microRNA-320a and insulin-like growth factor-1 receptor in early-onset preeclampsia patients

Liao, Guilian; Cheng, Dan-Ling; Li, Juan; Hu, Shaona

doi:10.1016/j.ejogrb.2021.06.032

Cited by 9 publications

(4 citation statements)

References 38 publications

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“…IGF1 plays a significant role in maintaining immune function, and IGF1R facilitates the differentiation of naïve CD4 + T cells into Th17 cells (DiToro et al, 2020). A study showed that IGF-1R was downregulated in the serum of individuals with PE (Liao et al, 2021). Decreased amounts of IGF1R were also found in the placentas of individuals with PE (Robajac et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Unveiling the Crucial Role of Pro-inflammatory Macrophages in the Immune Network Imbalance of the maternal-fetal interface with Preeclampsia

Fei,

Lu,

Shi

et al. 2024

Preprint

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Preeclampsia (PE), a major cause of maternal and perinatal mortality with highly heterogeneous causes and symptoms, is usually complicated by gestational diabetes mellitus (GDM). However, a comprehensive understanding of the immune microenvironment in the placenta of PE and the differences between PE and GDM is still lacking. In this study, Cytometry by time of flight (CyTOF) indicated that the frequencies of memory-like Th17 cells (CD45RA-CCR7+IL-17A+CD4+), memory-like CD8+T cells (CD45RA-CCR7+CD38+pAKTmidCD127low) and pro-inflam Macs (CD206-CD163-CD38midCD107alowCD86midHLA-DRmidCD14+) were increased, while the frequencies of CD69hiHeliosmidCD127midγδT cells, anti-inflam Macs (CD206+CD163-CD86midCD33+HLA-DR+) and granulocyte myeloid-derived suppressor cells (gMDSCs, CD11b+CD15hiHLA-DRlow) were decreased in the placenta of PE compared with that of NP, but not in that of GDM or GDM&PE. The pro-inflam Macs were positively correlated with memory-like Th17 cells and memory-like CD8+T cells but negatively correlated with gMDSCs. Single-cell RNA sequencing revealed that transferring the F480+CD206-pro-inflam Macs with a Folr2+Ccl7+Ccl8+C1qa+C1qb+C1qc+phenotype from the uterus of PE mice to normal pregnant mice induced the production of memory-like IL-17a+Rora+Il1r1+TNF+Cxcr6+S100a4+CD44+Th17 cells via IGF1-IGF1R, which contributed to the development and recurrence of PE. Pro-inflam Macs also induced the production of memory-like CD8+T cells but inhibited the production of Ly6g+S100a8+S100a9+Retnlg+Wfdc21+gMDSCs at the maternal-fetal interface, leading to PE-like symptoms in mice. In conclusion, this study revealed the PE-specific immune cell network, which was regulated by pro-inflam Macs, providing new ideas about the pathogenesis of PE.

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Section: Discussionmentioning

confidence: 99%

WITHDRAWN: Unveiling the Crucial Role of Pro-inflammatory Macrophages in the Immune Network Imbalance of the maternal-fetal interface with Preeclampsia

Fei,

Lu,

Shi

et al. 2024

Preprint

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“…Suggesting that miR-377-3p may be involved in the pathogenesis of PE and correlated with disease severity and neonatal prognosis. MiR-320a was shown to be upregulated in patients with early-onset PE and differentially expressed in severe early-onset PE than in mild early-onset PE, further suggesting that miR-320a expression could be related to the severity of early-onset PE [20].The results of the present study was similar to those of Liao et al In addition, this study also showed a positive correlation between miR-377-3p expression in plasma and placental tissues, suggesting that miR-377-3p expression levels in blood circulation could better re ect the expression of this miRNA in placental tissues.…”

Section: Discussionmentioning

confidence: 98%

Expression of miR-377-3p in preeclampsia and its effect on CCNG2

Niu,

Yu,

Wang

et al. 2024

Preprint

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Background To investigate the relationship between changes in miR-377-3p and cell cyclin G2 (CCNG2) expression and clinical features of pregnant women in preeclampsia (PE) and to further analyze the effect of miR-377-3p on CCNG2. Methods 120 pregnant subjects were recruited consisting of 70 PE and 50 healthy pregnancies. The expression of miR-377-3p and CCNG2 mRNA were detected through RT-qPCR, and the protein expression of CCNG2 was examined through Western Blot. The Pearson test was used to analyze the relationship between miR-377-3p and CCNG2 mRNA expression and clinical parameters related to pregnant women with PE, and the association between them. Dual luciferase assay to verify the effect of miR-377-3p on CCNG2. Results The expression of miR-377-3p in the peripheral plasma and placenta of pregnant women in the PE group was lower than that in the control group, and the expression levels of CCNG2 mRNA and protein were higher than that in the control group. Expression of miR-377-3p and CCNG2 mRNA in plasma and placenta of pregnant women with PE was correlated with systolic, diastolic blood pressure and neonatal birth weight. miR-377-3p and CCNG2 were consistently expressed in plasma and placental tissues, and their expressions were negatively correlated. Dual luciferase assay showed that miR-377-3p negatively regulated CCNG2 expression in HTR-8/SVneo cells. Conclusion Aberrant expression of miR-377-3p and CCNG2 in pregnant women with PE was associated with the severity of PE and its prognosis. It is hypothesized that miR-377-3p may be involved in the pathogenesis of PE through targeted inhibition of CCNG2 expression.

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“…The potential role of miR-92a-3p, miR-320a, and miR-16-5p in the pathogenesis of pre-eclampsia (PE) has been studied in several works, revealing that (i) intravascular inflammation occurs in PE as a sequence of Th1 polarization [41,42] through the targeting of GATA3 by upregulated miR-92a-3p, contained in vesicles of activated NK cells [43]; (ii) miR-320a overexpression observed in PE inhibits trophoblast cell invasion and causes anomalous placentation by targeting estrogen-related receptor-gamma [44], IL-4 [45], and IGF-1R [46]; (iii) in a PE rat model, the upregulation of miR-16-5p directly downregulates IGF-2 and provides inhibition of trophoblast cell viability and migration [47]. Thus, the involvement of the same miRNA molecules in different diseases necessitates the search for a unique combination of marker miRNAs that can differentiate one pregnancy complication from another.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Role of Cell-Free miRNAs in Identifying Placenta Accreta Spectrum during First-Trimester Screening

Timofeeva,

Fedorov,

Suhova

et al. 2024

IJMS

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Placenta accreta spectrum (PAS) is a severe complication of pregnancy associated with excessive invasion of cytotrophoblast cells at the sites of the endometrial–myometrial interface and the myometrium itself in cases of adherent (creta) and invasive (increta and percreta) forms, respectively. This leads to a high risk of massive blood loss, maternal hysterectomy, and preterm birth. Despite advancements in ultrasound protocols and found associations of alpha-fetoprotein, PAPP-A, hCG, PLGF, sFlt-1, IL-8, and IL-33 peripheral blood levels with PAS, there is a high need for an additional non-invasive test to improve the diagnostic accuracy and to select the real PAS from the suspected ones in the first-trimester screening. miRNA signatures of placental tissue, myometrium, and blood plasma from women with PAS in the third trimester of pregnancy, as well as miRNA profiles in exosomes from the blood serum of women in the first trimester with physiologically progressing pregnancy, complicated by PAS or pre-eclampsia, were obtained using deep sequencing. Two logistic regression models were constructed, both featuring statistically significant parameters related to the levels of miR-26a-5p, miR-17-5p, and miR-101-3p, quantified by real-time PCR in native blood serum. These models demonstrated 100% sensitivity in detecting PAS during the first pregnancy screening. These miRNAs were identified as specific markers for PAS, showing significant differences in their blood serum levels during the first trimester in the PAS group compared to those in physiological pregnancies, early- or late-onset pre-eclampsia groups. Furthermore, these miRNAs exhibited differential expression in the PAS placenta and/or myometrium in the third trimester and, according to data from the literature, control angiogenesis. Significant correlations were found between extracellular hsa-miR-101-3p and nuchal translucency thickness, hsa-miR-17-5p and uterine artery pulsatility index, and hsa-miR-26a-5p and hsa-miR-17-5p with PLGF. The developed test system for early non-invasive PAS diagnosis based on the blood serum level of extracellular miR-26a-5p, miR-17-5p, and miR-101-3p can serve as an auxiliary method for first-trimester screening of pregnant women, subject to validation with independent test samples.

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Clinical significance of microRNA-320a and insulin-like growth factor-1 receptor in early-onset preeclampsia patients

Cited by 9 publications

References 38 publications

WITHDRAWN: Unveiling the Crucial Role of Pro-inflammatory Macrophages in the Immune Network Imbalance of the maternal-fetal interface with Preeclampsia

WITHDRAWN: Unveiling the Crucial Role of Pro-inflammatory Macrophages in the Immune Network Imbalance of the maternal-fetal interface with Preeclampsia

Expression of miR-377-3p in preeclampsia and its effect on CCNG2

Diagnostic Role of Cell-Free miRNAs in Identifying Placenta Accreta Spectrum during First-Trimester Screening

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