Clinical significance of preoperative and postoperativecytokeratin 19messenger RNA level in peripheral blood of esophageal cancer patients

Abstract

The purpose of this study is to analyze the correlation between preoperative/postoperative Cytokeratin 19 (CK19) messenger RNA (mRNA) level in peripheral blood (PB) and the clinical significance in esophageal cancer patients with different clinicopathological factors. We detected the preoperative and postoperative CK19 mRNA level in the PB of 139 esophageal cancer patients who underwent complete resection and evaluated its clinical significance. We found that both the preoperative and postoperative CK19 mRNA level increased in the esophageal cancer patients with lymph node metastasis, relapse or distant metastasis compared with that in cancers without lymph node metastasis, relapse or distant metastasis. High postoperative CK19 mRNA levels indicate a short disease-free survival (DFS) for the whole cohort esophageal cancer patients, whereas the high preoperative CK19 mRNA levels only indicate a short DFS for the esophageal cancer patients with squamous cell carcinoma, TNM III stage, and lymph node metastasis. The dynamic change of CK19 mRNA levels could indicate the prognosis of esophageal cancer patients. The patients with decreasing CK19 mRNA level after surgery had good prognosis, and the patients with changeless CK19 mRNA level had poor prognosis. Taken together, CK19 mRNA levels could be a promising marker in assessing prognosis or assigning treatment for the esophageal cancer patients according to different clinicopathological factors.

Prognostic significance of preoperative and postoperative CK19 and CEA mRNA levels in peripheral blood of patients with gastric cardia cancer

“…Cytokeratins are components of epithelial cells and the intermediate filaments of epithelial cancer cells; these proteins are involved in the dynamic remodeling of tumor cells during invasion and metastasis. CK19, a tissue-specific marker for epithelial tumor micrometastasis, has been reported to be a prognostic marker in esophageal cancer[ 9 ], hepatocellular carcinoma[ 25 ], and lung cancer[ 21 ]. Based on the previously published data of CEA and CK19 in other malignancies, we further explored the relationships between pre- and post-CEA and CK19 mRNA levels with OS in patients with GCC.…”

“…The pelleted cells were resuspended in red blood cell lysis buffer (Solarbio) and centrifuged another two times. The remaining cells, which are karyocytes in PB, were washed with PBS, and the total cellular RNA isolation and cDNA synthesis were performed as previously described[ 9 ]. Total RNA was extracted from the cell lysate using TRIzol reagent (Invitrogen, Gaithersburg, MD, United States) according to the manufacturer’s instructions.…”

“…Cytokeratin 19 (CK19), which originates from epithelial cells, is a reliable tissue-specific marker for epithelial tumor micrometastasis and is highly expressed in the gastrointestinal tract but not in normal lymph node tissue or blood. The detection of CEA and CK19 expression has been used to examine tumor micrometastasis and predict the prognosis in GC and esophageal squamous cell carcinoma[ 8 , 9 ]. Nevertheless, there is no valid marker to predict the lymph node metastasis and prognosis in patients with GCC.…”

Clinicopathological and prognostic value of circulating tumor cells in esophageal carcinoma: a meta-analysis

“…All investigators finally agreed to include 33 eligible studies in our meta-analysis (Table 1). Among these, Seventeen studies (19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) were conducted on esophageal squamous cell carcinoma (ESCC), and nine (36)(37)(38)(39)(40)(41)(42)(43)(44) addressed esophageal adenocarcinoma cancer (EAC). Other 3) and no single study markedly changed the overall effect on the sensitivity analysis.…”

“…Nineteen studies (19)(20)(21)23,24,26,27,(30)(31)(32)(33)(34)(35)39,40,43,44,49,51) were included in this meta-analysis. High heterogeneity was shown among the studies (I 2 =83.4%, P=0.000).…”