Clinical significance of serum miR-196a in cervical intraepithelial neoplasia and cervical cancer

Abstract

ABSTRACT. Previous studies have reported that miR-196a is upregulated in cervical cancer tissues and cell lines. However, whether serum miR196a is increased in patients with cervical cancer or cervical intraepithelial neoplasia (CIN), and its potential clinical value remained unknown. In total, 105 cervical cancer patients, 86 CIN patients, and 50 healthy volunteers were recruited. Quantitative reverse transcription-polymerase chain reaction was performed to compare the serum levels miR-196a in all participants. The associations between serum miR-196a and CIN grade/ clinicopathological parameters of cervical cancer were also examined. A survival analysis was performed using the Kaplan-Meier method. Univariate and multivariate analyses were conducted to explore the independent risk factors for cervical cancer. Our results revealed that serum miR196a levels were higher in patients with cervical cancer (P < 0.01) and CIN (P < 0.05) compared to those in healthy controls. Serum miR-196a was associated with CIN grade and various cervical cancer parameters including tumor size (P = 0.031), lymph node metastasis (P = 0.018),

Use of Mature miRNA Strand Selection in miRNAs Families in Cervical Cancer Development

“…Based on miRNA expression, it is possible to know the stage of cancer progression, making them ideal molecular markers for diagnosis. Up-regulation of miR-155 [21] and miR-196a [114] and down-regulation of miR-26a [128], miR-125a [101], and miR-126 [129] are associated with poor survival of patients with cervical cancer predicting relapse and survival of patients. Regarding miRNA therapy, it should be taken into account that miRNA overexpression or inhibition could present RNAi off-target [130].…”

“…Interestingly, the 3p strand is not annotated for miR-196a [20] (Figure 3). miR-196a is overexpressed in serum [114], Cervical Intraepithelial Neoplasia (CIN) 2-3 and cervical cancer tissues inducing proliferation and migration, diminishing mRNA and protein of Netrin 4 (NTN4) via 3′UTR. This protein is a member of Netrin family that promotes neurite growth and cell branching [115].…”

MicroRNAs in gynecological cancers: Small molecules with big implications

“…A correlation between serum miR-196a levels and the tumor size, grade, lymph node metastasis and FIGO stage was suggested by Liu and coworkers [304]. Based on multivariate analysis, authors reported that higher serum miR-196a levels in CC patients were an independent predictor for poor survival.…”

“…A recent study by Coimbra and coworkers found that the expression levels miR-203 and of ΔNp63 mRNA positively correlate with each other in CC tissues, and could serve as a promising tool for the screening of CC [303]. Liu et al suggested the use of miR-196 as a diagnostic marker based on their observation that serum levels of miR-196a are elevated in CC patients as well as in cervical intraepithelial neoplasia (CIN) [304]. Altogether, these reports underscore the potential of miRNAs to be used as valuable tools for discriminating GCs from normal cases and classifying the tumor stage and grade, either alone, or in combination with other biomarkers.…”

Effect of miRNA-203 on cervical cancer cells and its underlying mechanism

“…Studies have shown that miRNA are abnormally expressed in cancer. miRNA participate in the tumor cell differentiation, proliferation, and apoptosis processes (Gaca et al, 2012;Ji et al, 2012;Jin et al, 2013;Huang et al, 2014;Liu et al, 2015). A previous report suggested that miRNA-203 plays an important role in the pathogenesis of many types of cancer (Kapinas et al, 2015).…”