Clinical Spectrum of Leishmaniasis

Pearson, Richard D.; Sousa, Anastácio de Queiroz

doi:10.1093/clinids/22.1.1

Cited by 466 publications

(344 citation statements)

References 41 publications

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“…Clinical symptoms result from replication of the parasites in macrophages of the dermis, naso-oropharyngeal mucosa, and the mononuclear phagocyte system, respectively (reviewed in Refs. [1][2][3]. Leishmaniasis is prevalent in four continents and is considered by the World Health Organization to be one of the top six most important human parasitic diseases.…”

Section: Identification and Characterization Of T Cell-stimulating Anmentioning

confidence: 99%

“…In 1990, estimates by the World Health Organization indicated that ϳ350 million people were at risk of acquiring leishmaniasis and that 12 million were currently infected (4). In a recent epidemic of visceral leishmaniasis (VL) 3 in southern Sudan, Medicins San Frontièrers estimated that the excess mortality has been about 100,000 deaths among about 300,000 people at risk (5).…”

Section: Identification and Characterization Of T Cell-stimulating Anmentioning

confidence: 99%

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Identification and Characterization of T Cell-Stimulating Antigens from Leishmania by CD4 T Cell Expression Cloning

Probst

Stromberg

Ghalib

et al. 2001

The Journal of Immunology

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Persistent immunity against Leishmania infections in humans is mediated predominantly by CD4+ T cells of the Th1 phenotype. Herein we report the expression cloning of eight Leishmania Ags using parasite-specific T cell lines derived from an immune donor. The Ags identified by this technique include the flagellar proteins α- and β-tubulin, histone H2b, ribosomal protein S4, malate dehydrogenase, and elongation factor 2, as well as two novel parasite proteins. None of these proteins have been previously reported as T cell-stimulating Ags from Leishmania. β-tubulin-specific T cell clones generated against Leishmania major amastigotes responded to Leishmania-infected macrophages and dendritic cells. IFN-γ enzyme-linked immunospot analysis demonstrated the presence of T cells specific for several of these Ags in PBMC from self-healing cutaneous leishmaniasis patients infected with either Leishmania tropica or L. major. The responses elicited by Leishmania histone H2b were particularly striking in terms of frequency of histone-specific T cells in PBMC (1 T cell of 6000 PBMC) as well as the percentage of responding donors (86%, 6 of 7). Ags identified by T cells from immune donors might constitute potential vaccine candidates for leishmaniasis.

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Section: Identification and Characterization Of T Cell-stimulating Anmentioning

confidence: 99%

Section: Identification and Characterization Of T Cell-stimulating Anmentioning

confidence: 99%

Identification and Characterization of T Cell-Stimulating Antigens from Leishmania by CD4 T Cell Expression Cloning

Probst

Stromberg

Ghalib

et al. 2001

The Journal of Immunology

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“…The clinical forms of leishmaniasis depend on the association among the virulent characteristics of the infective Leishmania and host susceptibility to the illnesses (Pearson and Souza, 1996). Leishmaniasis is categorized as cutaneous, mucocutaneous, diffuse cutaneous, and visceral.…”

Section: Introductionmentioning

confidence: 99%

Crude Ethanolic Extract, Lignoid Fraction and Yangambin from Ocotea duckei (Lauraceae) Show Antileishmanial Activity

Monte

Barbosa

Sousa

et al. 2007

Zeitschrift Für Naturforschung C

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Crude ethanolic extract, lignoid fraction and the purified compound yangambin were obtained from Ocotea duckei (Lauraceae) and their antileishmanial activity was tested against promastigote forms of Leishmania chagasi and Leishmania amazonensis cultivated in Schneider medium, supplemented with 20% of fetal bovine serum. All substances presented antileishmanial activity with IC 50 values of 135.7 μg/mL for the crude ethanolic extract, 26.5 μg/mL for the lignoid fraction and 49.0 μg/mL for yangambin on L. chagasi. For L. amazonensis the IC 50 values were 143.7 μg/mL, 48.2 μg/mL and 64.9 μg/mL for the crude ethanolic extract, the lignoid fraction, and the purified compound yangambin, respectively. The crude ethanolic extract, lignoid fraction, and yangambin caused an inhibition higher than Glucantime " , a reference drug used for the treatment of leishmaniasis.

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“…1 Apresentando-se com febre, perda de peso gradual, esplenomegalia, hipergamaglobulinemia e pancitopenia, esta doença é fatal se não diagnosticada e tratada. 2 Portanto, um método simples de diagnóstico é essencial. De mesma importância é a validade do diagnóstico, uma vez que, a terapia anti-leishmania frequentemente resulta em significante mortalidade e morbidade.…”

Section: Introductionunclassified

Avaliação do teste rápido utilizando o antígeno recombinante K39 no diagnóstico da leishmaniose visceral no Brasil

Lemos

Carvalho

Corey

et al. 2003

Rev. Soc. Bras. Med. Trop.

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Este estudo avaliou o desempenho teste rápido de imunocromatografia utilizando o antígeno rK39 (Kalazar Detected® InBios International, Seattle, WA, USA) e um teste de ELISA utilizando antígeno total, para o diagnóstico da leishmaniose visceral (LV) em 128 pacientes com diagnóstico parasitológico da doença. Como controle foram incluídos soros de 10 indivíduos saudáveis e de 50 pacientes portadores de outras infecções como: malária (10), hanseníase (9), doença de Chagas (10), tuberculose (10) e leishmaniose cutânea (11). A sensibilidade do teste rápido com antígeno rK39 e ELISA foram 90% e 89% respectivamente, enquanto a especificidade foi 100% e 98% respectivamente. Nossos dados portanto, confirmam a eficiência do teste rápido com rK39 no diagnóstico da LV

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Clinical Spectrum of Leishmaniasis

Cited by 466 publications

References 41 publications

Identification and Characterization of T Cell-Stimulating Antigens from Leishmania by CD4 T Cell Expression Cloning

Identification and Characterization of T Cell-Stimulating Antigens from Leishmania by CD4 T Cell Expression Cloning

Crude Ethanolic Extract, Lignoid Fraction and Yangambin from Ocotea duckei (Lauraceae) Show Antileishmanial Activity

Avaliação do teste rápido utilizando o antígeno recombinante K39 no diagnóstico da leishmaniose visceral no Brasil

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