Clinical strains of Streptococcus agalactiae carry two different variants of pathogenicity island XII

Kuleshevich, Eugenia; Ferretti, Joseph J.; Sanches, Ilda Santos; Balasubramanian, N; Spellerberg, Barbara; Efstratiou, Androulla; Kriz, Paula; Grabovskaya, K. B.; Arjanova, O. N.; Savitcheva, A. M.; Shevchenko, V.A.; Рысев, А. П.; Suvorov, Alexander

doi:10.1007/s12223-017-0509-8

Cited by 2 publications

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References 23 publications

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“…The streptococcal genes encode exotoxins, superantigens, and streptodornases, which are responsible for GAS virulence and pathogenesis ( Igwe et al, 2003 ; Pires et al, 2009 ). Most of the diseases caused by GAS are probably due to the diversity of the virulent gene’s products mainly encoded by mobile genetic elements, i.e., prophages ( Walker et al, 2014 ; Kuleshevich et al, 2017 ). Furthermore, streptococcal pyrogenic exotoxins (s peA or speC ) and streptococcal superantigen ( ssa ) are bacteriophage encoded virulence factors that are associated with invasive diseases ( McCormick et al, 2001 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Docking of speB Gene Encoding Cysteine Protease With Antibiotic Interaction in Streptococcus pyogenes NBMKU12 From the Clinical Isolates

et al. 2018

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Streptococcus pyogenes causes a variety of diseases ranging from mild diseases to severe invasive infections which result in significant morbidity and mortality. This study focuses on the antibiotic resistance of S. pyogenes and their interaction with cysteine protease. Around 36 beta-hemolytic isolates were collected from the clinical lab, of which seven isolates (19.4%) were identified as Streptococcus pyogenes. One of the seven isolates was collected from a urinary tract infection, which was identified by antibody agglutination and MALTI-TOF-MS, and it is designated as S. pyogenes NBMKU12. Around 8.3 to 66.6 % of the isolates were found to be resistant to one or more antimicrobial agents, especially, penicillin-G resistance was exhibited by 29.1% of the isolates. In the NBMKU12 isolate, the beta lactem (TEM) gene was detected among the 13 antibiotic genes for which it was tested. Furthermore, when analysis for presence of 13 virulence genes were carried out in NBMKU12 isolate, only speJ and speB were detected. The speB (streptococcal pyrogenic exotoxin B) encoding cysteine protease gene was cloned. This was followed by performing DNA sequencing to understand the putative cysteine protease interaction with antibiotics, inhibitors, and substrate. The speB gene consists of 1197 nucleotides and encodes a protein with multiple domains, including a signal peptide (aa 1–22), an inhibitor region (aa 27–156), and a catalytic cysteine domain (aa 160–367). The signal peptide cleavage site is predicted between Ala22 and Asn23. The putative 398 amino acid residues were found to have a theoretical pI of 8.76 and a molecular mass of 43,204.36 Da. The tested culture supernatants of NBMKU12 isolate exhibited the proteolytic activity against casein, papaya and pineapple used as substrates. The proteolytic activity suggests the expression of speB gene. Molecular docking analysis of cysteine protease showed that erythromycin (bond length 2.41 Å), followed by chloramphenicol (2.51 Å), exhibited a strong interaction; while penicillin-G (3.24 Å) exhibited a weak interaction, and this factor could be considered as a cause for penicillin-G resistance. The present study contributes to a better understanding of speB gene encoding cysteine protease, antibiotic resistance, and their interaction in the isolate, S. pyogenes NBMKU12. The antibiotics and cysteine protease interaction study confirms the resistance or sensitivity of S. pyogenes. Hence, it could be hypothesized that the isolate NBMKU12 is resistant to most of the tested antibiotics, and this resistance might be a cause for mutation.

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Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Docking of speB Gene Encoding Cysteine Protease With Antibiotic Interaction in Streptococcus pyogenes NBMKU12 From the Clinical Isolates

et al. 2018

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Russian strains of group B streptococci are different in the content and organization of the PAI-A and PAI-A1 pathogenicity islands

et al. 2021

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Group B streptococci, or Streptococcus agalactiae, are the major cause of severe diseases in newborns and adults. The PAI-A and PAI-A1 pathogenicity islands containing the sspB1 and sspB1a genes, respectively, were found among group B streptococci mobile genetic elements. The presence of sspB genes correlates with urogenital tract infections. The aim of this study was to determine the frequency of group B streptococci strains with the PAI-A and PAI-A1 pathogenicity islands, circulating in Moscow, in comparison with strains from St. Petersburg. The sspB1 gene, and hence the PAI-A pathogenicity island, was not found in the genomes of strains from Moscow. The frequency of the sspB1a gene and the PAI-A1 pathogenicity island in the genomes of clinical strains was three times higher than in the genomes of colonizing strains. Thus, it can be assumed that the genes of the sspB family are more specific of group B streptococci colonizing pregnant women and newborns.

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Clinical strains of Streptococcus agalactiae carry two different variants of pathogenicity island XII

Cited by 2 publications

References 23 publications

Molecular Cloning and Docking of speB Gene Encoding Cysteine Protease With Antibiotic Interaction in Streptococcus pyogenes NBMKU12 From the Clinical Isolates

Molecular Cloning and Docking of speB Gene Encoding Cysteine Protease With Antibiotic Interaction in Streptococcus pyogenes NBMKU12 From the Clinical Isolates

Russian strains of group B streptococci are different in the content and organization of the PAI-A and PAI-A1 pathogenicity islands

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