Clinical Trials of Probiotics in Patients With Irritable Bowel Syndrome: Some Points to Consider

Quigley, Eamonn Martin

doi:10.5056/jnm22012

Cited by 13 publications

(11 citation statements)

References 36 publications

(35 reference statements)

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“…The therapeutic impact of probiotics on glycaemic control has been the subject of various investigations in humans. Recent clinical studies have produced encouraging outcomes, which emphasize the necessity of a thorough systematic review and meta-analysis of these results ( Markowiak and Ślizewska, 2017 ; Kim et al, 2019 ; Dudek-Wicher et al, 2020 ; Montassier et al, 2021 ; Mullish et al, 2021 ; Quigley, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of lactic acid bacteria with potential probiotic activity and further investigation of their activity by α-amylase and α-glucosidase inhibitions of fermented batters

et al. 2023

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Probiotic microbiota plays a vital role in gastrointestinal health and possesses other beneficial attributes such as antimicrobial and antibiotic agents along with a significant role in the management of diabetes. The present study identifies the probiotic potential of Lactobacillus spp. isolated from three traditionally fermented foods namely, jalebi, medhu vada, and kallappam batters at biochemical, physiological, and molecular levels. By 16S rRNA gene amplification and sequencing, the isolates were identified. A similarity of >98% to Lacticaseibacillus rhamnosus RAMULAB13, Lactiplantibacillus plantarum RAMULAB14, Lactiplantibacillus pentosus RAMULAB15, Lacticaseibacillus paracasei RAMULAB16, Lacticaseibacillus casei RAMULAB17, Lacticaseibacillus casei RAMULAB20, and Lacticaseibacillus paracasei RAMULAB21 was suggested when searched for homology using NCBI database. Utilizing the cell-free supernatant (CS), intact cells (IC), and cell-free extract (CE) of the isolates, inhibitory potential activity against the carbohydrate hydrolyzing enzymes α-glucosidase and α-amylase was assessed. CS, CE, and IC of the isolates had a varying capability of inhibition against α-glucosidase (15.08 to 59.55%) and α-amylase (18.79 to 63.42%) enzymes. To assess the probiotic potential of seven isolates, various preliminary characteristics were examined. All the isolates exhibited substantial tolerance toward gastrointestinal conditions and also demonstrated the highest survival rate (> 99%), hydrophobicity (> 65%), aggregation (> 76%), adherence to HT-29 cells (> 84%), and chicken crop epithelial cells suggesting that the isolates had a high probiotic attribute. Additionally, the strains showed remarkable results in safety assessment assays (DNase and hemolytic), and antibacterial and antibiotic evaluations. The study concludes that the lactic acid bacteria (LAB) characterized possesses outstanding probiotic properties and has antidiabetic effects. In order to obtain various health advantages, LAB can be utilized as probiotic supplements.

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Section: Discussionmentioning

confidence: 99%

Isolation and characterization of lactic acid bacteria with potential probiotic activity and further investigation of their activity by α-amylase and α-glucosidase inhibitions of fermented batters

et al. 2023

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“…To avoid these problems in probiotic clinical trials, experts recommend selecting an appropriate research design in advance by considering the confounding factors above. 6 Although a parallel design is considered gold standards, a crossover design may reveal more accurate results if underlying gut microbiota is the main determining factor for the effectiveness of probiotics. 6 , 7 However, the crossover design is discouraged for the unknown carry-over effect of probiotics.…”

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confidence: 99%

“… 6 Although a parallel design is considered gold standards, a crossover design may reveal more accurate results if underlying gut microbiota is the main determining factor for the effectiveness of probiotics. 6 , 7 However, the crossover design is discouraged for the unknown carry-over effect of probiotics. 6 For this issue, Tremblay’s study 4 provided valuable insights that determining the optimal washout period for each study by considering both probiotic strain and the host-specific parameters is ideal, rather than having a 2-5-week washout period without rationale.…”

mentioning

confidence: 99%

“… 6 , 7 However, the crossover design is discouraged for the unknown carry-over effect of probiotics. 6 For this issue, Tremblay’s study 4 provided valuable insights that determining the optimal washout period for each study by considering both probiotic strain and the host-specific parameters is ideal, rather than having a 2-5-week washout period without rationale.…”

mentioning

confidence: 99%

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The Long and Winding Road to the Best Clinical Research and Personalized Therapy With Probiotics: Do Not Forget Host Physiology

Kim¹,

Choi

2023

J Neurogastroenterol Motil

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The human gut microbiota is composed of numerous bacteria similar in number to human cells, and each individual has their own gut microbiota status. 1 Research on human gut microbiota has grown exponentially in the past 2 decades and has demonstrated that gut microbiota is crucial for maintaining human health. At the same time, it has also been known that dysbiosis is related to many diseases. However, no gut microbiota-based therapy is available in clinical practice other than fecal microbiota transplantation for Clostridioides difficile infection. Only probiotics have been used for more than 100 years in human life, and some antibiotics are onhand as a modality to improve gut dysbiosis.Multiple factors, including delivery mode, breastfeeding, diet, medication, exercise, aging, stress, and infection, can influence the gut microbiota. 2 The widespread inter-individual heterogeneity of gut microbiota composition makes gut microbiota research inconsistent. 3 Similarly, when probiotics, living microorganisms by definition, are administered and act on the gut, they can be affected by various factors and each individual's gut microbiota. In this issue of the Journal of Neurogastroenterology and Motility, Tremblay et al 4 demonstrated the relationship between the persistence of probiotics JNM

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“…The possibility to influence the homeostasis of neuroactive molecules such as 5-HT and GABA by means of microbiota-based treatment to attenuate gut and central nervous system symptoms related to IBS, has been extensively described in the review by Chen et al, which underlines the potential of dopamine and histamine as additional amine neurotransmitters that have received limited attention until now (4). However, manipulation of the indigenous microbiota has been shown to be an effective approach primarily on animal-based investigations since clinical evidence is still quite poor with variable results on disease outcomes mainly due to patient heterogeneity (5). Neuroendocrine signals involving hormones, such as sex steroids, along the microbiota-gut brain axis may also have a pathogenetic role in IBS and contribute to the sexual dimorphism, which represents a main feature of IBS, with females more prone to develop the disorder with respect to males.…”

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confidence: 99%

Editorial: Neuroendocrine signalling pathways along the microbiota-gut-brain axis in functional gut disorders

2022

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Editorial on the Research TopicNeuroendocrine signalling pathways along the microbiota-gut-brain axis in functional gut disorders Irritable bowel syndrome (IBS) is the most common among functional gastrointestinal disorders (FGIDs) characterized by non-structural symptoms that destabilize the patient's quality of life with increasing incidence in industrialized countries. Although the exact etiopathogenesis remains unknown, FGDIs develop as a consequence of miscellaneous factors, such as genetic, environmental, immune, inflammatory, neurological and psychological triggers, in addition to visceral hypersensitivity and postinfectious events. The interplay between an altered gut physiology and psychological factors is suggested to depend upon the existence of bidirectional communication pathways where the gut and the brain influence each other along the so-called "gut-brain axis". This communication axis involves the gut microbiota, which plays a pivotal role in maintaining local, systemic and gut-brain homeostasis, by secreting several neuroactive molecules, hormones and metabolites. From the seminal discovery, about 30 years ago, of noradrenaline and adrenaline as neuroendocrine molecules along the microbiota-gut-brain axis, it is now ascertained that several other neuroactive molecules are produced by microbes inhabiting the human gut. Tryptophanderived metabolites, such as serotonin (5-hydroxytryptamine (5-HT)), kynurenine and indoles, neurotransmitters, such as glutamate, g-aminobutyric acid (GABA) and acetylcholine, neuroactive short-and medium-chain fatty acids, secondary bile acids, branch-chain aminoacids are among the most relevant molecules produced by saprophytic bacteria (1). The present Research Topic highlights the more recent advances on the involvement of neuroendocrine signalling pathways along the microbiota-gut-brain axis in the pathogenesis of FGIDs.In this context, the review of Layunta et al. defines 5-HT, a fundamental neurotransmitter/ neuromodulator involved in the control of both gut physiology and central nervous systemFrontiers in Endocrinology frontiersin.org 01

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Clinical Trials of Probiotics in Patients With Irritable Bowel Syndrome: Some Points to Consider

Abstract: published online; they have not yet been assigned to a journal issue. When these articles are published in an issue, they will be removed. Online First articles are copy-edited, typeset and approved by the authors before publication.

Cited by 13 publications

References 36 publications

Isolation and characterization of lactic acid bacteria with potential probiotic activity and further investigation of their activity by α-amylase and α-glucosidase inhibitions of fermented batters

Isolation and characterization of lactic acid bacteria with potential probiotic activity and further investigation of their activity by α-amylase and α-glucosidase inhibitions of fermented batters

The Long and Winding Road to the Best Clinical Research and Personalized Therapy With Probiotics: Do Not Forget Host Physiology

Editorial: Neuroendocrine signalling pathways along the microbiota-gut-brain axis in functional gut disorders

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