Clinical use of autologous adipose-derived stromal vascular fraction cell injections for hip osteoarthritis

Onoi, Yuma; Matsumoto, Tomoyuki; Sobajima, Satoshi; Tsubosaka, Masanori; Hayashi, Shinya; Matsushita, Takehiko; Iwaguro, Hideki; Kuroda, Ryosuke

doi:10.1016/j.reth.2023.06.006

Cited by 7 publications

(7 citation statements)

References 40 publications

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“…SVF [22][23][24] Harvested from adipose tissue; packed with cellular entities to facilitate healing.…”

Section: Stimulation Technique Mechanism Of Action Application Method...mentioning

confidence: 99%

“…Moreover, these cells are known for their angiogenic and immunomodulatory capabilities, primarily due to their secretion of growth factors and cytokines [23]. While the regenerative and reparative capacities can be traced back to the ADSCs, the immune cells within SVF contribute to the immunomodulatory effects, essential for tissue repair and regeneration [24]. Over the following decades, this recognition spiraled into a flurry of research investigating the regenerative potential of SVF, spurred by its accessibility and abundant stem cell content.…”

Section: Introductionmentioning

confidence: 99%

“…Through immune modulation, SVF, with its composition of immune cells, refines inflammatory responses, mitigating excessive inflammation and creating a favorable environment conducive to healing. SVF promotes angiogenesis, ensuring the formation of an integrated vascular network within the healing bone, which is crucial for the supply of essential nutrients and oxygen, and is crucial for optimal bone regeneration and pain reduction [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

“…Comparative overview of various bone stimulation modalities[10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26].…”

mentioning

confidence: 99%

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Comparative Analysis of Stromal Vascular Fraction and Alternative Mechanisms in Bone Fracture Stimulation to Bridge the Gap between Nature and Technological Advancement: A Systematic Review

Goncharov,

Koval,

Nikolaevich Bezuglov

et al. 2024

Biomedicines

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Background: Various stimulation methods, including electrical, ultrasound, mechanical, and biological interventions, are explored, each leveraging intricate cellular and molecular dynamics to expedite healing. The advent of stromal vascular fraction (SVF) marks a significant stride, offering multifarious benefits in bone healing, from enhanced bone formation to optimal vascular integration, drawing a harmonious balance between innate mechanisms and scientific advancements. Methods: This systematic review was conducted focusing on literature from 2016 to 2023 and encompassing various bone healing stimulation mechanisms like SVF, electrical, ultrasound, and mechanical stimulation. The extracted data underwent meticulous synthesis and analysis, emphasizing comparative evaluations of mechanisms, applications, and outcomes of each intervention. Results: The reviewed studies reveal the potential of SVF in bone fracture healing, with its regenerative and anti-inflammatory effects. The purification of SVF is crucial for safe therapeutic use. Characterization involves flow cytometry and microscopy. Studies show SVF’s efficacy in bone regeneration, versatility in various contexts, and potential for clinical use. SVF appears superior to electrical, ultrasound, and mechanical stimulation, with low complications. Conclusions: This review compares bone healing methods, including SVF. It provides valuable insights into SVF’s potential for bone regeneration. However, due to limited human studies and potential bias, cautious interpretation is necessary. Further research is essential to validate these findings and determine the optimal SVF applications in bone healing.

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“…SVF [22][23][24] Harvested from adipose tissue; packed with cellular entities to facilitate healing.…”

Section: Stimulation Technique Mechanism Of Action Application Method...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Comparative overview of various bone stimulation modalities[10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26].…”

mentioning

confidence: 99%

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Comparative Analysis of Stromal Vascular Fraction and Alternative Mechanisms in Bone Fracture Stimulation to Bridge the Gap between Nature and Technological Advancement: A Systematic Review

Goncharov,

Koval,

Nikolaevich Bezuglov

et al. 2024

Biomedicines

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show abstract

“…The SVF has been reported to be as effective as ADSCs in treating chronic lower-limb ulcers, ischemic cardiomyopathy, fat grafting, limb ischemia, and nerve regeneration [8][9][10][11]. Moreover, intra-articular injections of SVF can improve pain and joint function in OA, showing therapeutic effects comparable to those of ADSCs [12][13][14][15]. However, the proportion of ADSCs in the SVF is only approximately 1-16% [16], and the mechanisms underlying the effects of the SVF on chondrocytes remain unclear.…”

mentioning

confidence: 99%

Human uncultured adipose-derived stromal vascular fraction shows therapeutic potential against osteoarthritis in immunodeficient rats by enhancing anabolism-promoting and catabolism-inhibiting effects via direct effects of transplanted M2 macrophages

Onoi,

Matsumoto,

Anjiki

et al. 2024

Preprint

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Background The uncultured adipose-derived stromal vascular fraction (SVF), consisting of adipose-derived stromal cells (ADSCs), M2 macrophages (M2Φ) and others, has shown therapeutic potential against osteoarthritis (OA), however, the mechanisms underlying its therapeutic effects remain unclear. Therefore, this study investigated the effects of the SVF on OA in a human–immunodeficient rat xenotransplantation model. Methods OA model was induced in the knees of female immunodeficient rats by destabilization of the medial meniscus. Immediately after the surgery, human SVF (1 × 105), ADSCs (1 × 104), or phosphate buffered saline as a control group were transplanted into the knees. At 4 and 8 weeks postoperatively, OA progression and synovitis were analyzed by macroscopic and histological analyses, and the expression of collagen II, SOX9, MMP-13, ADAMTS-5, F4/80, CD86 (M1), CD163 (M2), and human nuclear antigen (hNA) were evaluated immunohistochemically. In vitro, flow cytometry was performed to collect CD163-positive cells as M2Φ from the SVF. Chondrocyte pellets (1×105) were co-cultured with SVF (1×105), M2Φ (1×104), and ADSCs (1×104) or alone as a control group, and the pellet size was compared. TGF-β, IL-10 and MMP-13 concentrations in the medium were evaluated using enzyme-linked immunosorbent assay. Results In comparison with the control and ADSC groups, the SVF group showed significantly slower OA progression and less synovitis with higher expression of collagen II and SOX9, lower expression of MMP-13 and ADAMTS-5, and lower F4/80 and M1/M2 ratio in the synovium. Only the SVF group showed partial expression of hNA-, CD163-, and F4/80-positive cells in the rat synovium. In vitro, the SVF, M2Φ, ADSC and control groups, in that order, showed larger pellet sizes, higher TGF-β and IL-10, and lower MMP-13 concentrations. Conclusions The M2Φ in the transplanted SVF directly affected recipient tissue, enhancing the secretion of growth factors and chondrocyte-protecting cytokines, and partially improving chondrocytes and joint homeostasis. These findings indicate that the SVF is as an effective option for regenerative therapy for OA, with mechanisms different from those of ADSCs.

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Management of hip osteoarthritis: harnessing the potential of mesenchymal stem cells—a systematic review

Giorgino,

Alessandri Bonetti,

Migliorini

et al. 2024

Eur J Orthop Surg Traumatol

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Introduction Hip osteoarthritis (OA) is a prevalent and debilitating condition, necessitating effective and safe treatment options. This systematic review aims to explore the potential of intra-articular mesenchymal stem cell (MSC) infiltrations as a therapeutic approach for hip OA. Methods Following PRISMA guidelines, a systematic review was conducted, encompassing PubMed, Embase, and Cochrane Library databases. Inclusion criteria involved studies focusing on intra-articular MSC injections in patients with hip OA and reporting pain relief as an outcome measure. Quality assessment utilized the Newcastle–Ottawa scale and methodological index for non-randomized studies. Results Ten studies were included in the review, exhibiting varied designs and sample sizes (316 patients). Outcome measures consisted of cartilage repair assessed through MRI and radiographies, pain scores (WOMAC, VAS, NRS), and functional improvements (HOS-ADL, OHS, FRI, PDQQ, LEFS). The studies reported favorable improvements in functional scores, pain relief, and cartilage repair/radiographic findings, with minimal reported adverse events. Conclusions Intra-articular MSC infiltrations demonstrate promise as an effective and safe therapeutic intervention for managing hip OA, offering pain relief and functional enhancements. Nevertheless, limited high-quality studies and outcome measure variations underscore the need for further research to establish definitive treatment guidelines. Future investigations should address optimal MSC utilization, long-term outcomes, and potential complications to ensure the success of MSC-based therapies for hip OA management, ultimately improving patient outcomes. The findings provide valuable insights into the potential of MSC-based treatments for hip OA, advocating further rigorous research in this field. Trial Registration The protocol was registered on PROSPERO database (CRD42023436973).

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Clinical use of autologous adipose-derived stromal vascular fraction cell injections for hip osteoarthritis

Cited by 7 publications

References 40 publications

Comparative Analysis of Stromal Vascular Fraction and Alternative Mechanisms in Bone Fracture Stimulation to Bridge the Gap between Nature and Technological Advancement: A Systematic Review

Comparative Analysis of Stromal Vascular Fraction and Alternative Mechanisms in Bone Fracture Stimulation to Bridge the Gap between Nature and Technological Advancement: A Systematic Review

Human uncultured adipose-derived stromal vascular fraction shows therapeutic potential against osteoarthritis in immunodeficient rats by enhancing anabolism-promoting and catabolism-inhibiting effects via direct effects of transplanted M2 macrophages

Management of hip osteoarthritis: harnessing the potential of mesenchymal stem cells—a systematic review

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