Clinical Usefulness of Combined Measurements of Serum Soluble Transferrin Receptor Levels and Serum Interleukin-18 Levels at Determination of Serum KL-6 Levels in Haematologic Malignancies

Takubo, Takayuki; Kumura, Takeo; Nakao, Takafumi; Nakamae, Hirohisa; Aoyama, Yasutaka; Nishiki, Saori; Kinoshita, Y.; Koh, Ki-Ryang; Ohta, Kensuke; Yamane, Takahisa; Hino, Masayuki; Kamitani, T; Tatsumi, Noriyuki

doi:10.1159/000039750

Cited by 12 publications

(12 citation statements)

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“…The data found in literature also indicate that higher, normal or lower values of sTfR level may be registered in MDS patients, reflecting various models of erythropoiesis that may be found in these patients [25]. In 14.3% of patients with multiple myeloma we found higher sTfR levels, while 85.7% had the normal values, that being in accordance with the literature [24]. The NHL patients had higher sTfR levels in 27.3% of cases, although all of them suffered from the active disease.…”

Section: Discussionsupporting

confidence: 91%

“…For that reason, it may be considered as the marker of the disease progression in CLL patients. In patients with acute leukemia types, sTfR values are generally lower or normal according to the literature data [24], so that higher sTfR values could indicate the associated IDA. We found lower or normal values of sTfR in 90.9% patients with acute leukemia.…”

Section: Discussionmentioning

confidence: 95%

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The significance of soluble transferrin receptors in diagnosing iron deficiency anemia

Tijanić

Vučić

Golubovic

2015

Revista Romana De Medicina De Laborator

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 95%

The significance of soluble transferrin receptors in diagnosing iron deficiency anemia

Tijanić

Vučić

Golubovic

2015

Revista Romana De Medicina De Laborator

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“…Appropriate sTfR levels for the degree of anemia could in fact result from an inadequately small amount of erythropoietic cell-derived sTfR with an exaggerated release by malignant lymphocytes. In patients with high-grade non-Hodgkin's lymphoma, elevated sTfR levels have been observed, which correlate with the tumor burden and clinical stage and normalize in patients responding to chemotherapy [135,136]. In conclusion, sTfR derived from tumor cells do not contribute significantly to overall sTfR levels, with the possible exception of non-Hodgkin's lymphoma, CLL and hepatocellular carcinoma.…”

Section: Soluble Tfr As a Tumor Markermentioning

confidence: 87%

“…Levels in acute leukemia have been found to be decreased at diagnosis and during chemotherapy [12,16,130,135]. Patients with many lymphoid malignancies, including hairy cell leukemia and multiple myeloma, have been found to have normal sTfR values, but this represents relative marrow failure for the degree of anemia [12,18,130,135].…”

Section: Soluble Tfr As a Tumor Markermentioning

confidence: 99%

Soluble transferrin receptor for the evaluation of erythropoiesis and iron status

Béguin

2003

Clinica Chimica Acta

398

316

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Iron transport in the plasma is carried out by transferrin, which donates iron to cells through its interaction with a specific membrane receptor, the transferrin receptor (TfR). A soluble form of the TfR (sTfR) has been identified in animal and human serum. Soluble TfR is a truncated monomer of tissue receptor, lacking its first 100 amino acids, which circulates in the form of a complex of transferrin and its receptor. The erythroblasts rather than reticulocytes are the main source of serum sTfR. Serum sTfR levels average 5.0 F 1.0 mg/l in normal subjects but the various commercial assays give disparate values because of the lack of an international standard. The most important determinant of sTfR levels appears to be marrow erythropoietic activity which can cause variations up to 8 times below and up to 20 times above average normal values. Soluble TfR levels are decreased in situations characterized by diminished erythropoietic activity, and are increased when erythropoiesis is stimulated by hemolysis or ineffective erythropoiesis. Measurements of sTfR are very helpful to investigate the pathophysiology of anemia, quantitatively evaluating the absolute rate of erythropoiesis and the adequacy of marrow proliferative capacity for any given degree of anemia, and to monitor the erythropoietic response to various forms of therapy, in particular allowing to predict response early when changes in hemoglobin are not yet apparent. Iron status also influences sTfR levels, which are considerably elevated in iron deficiency anemia but remain normal in the anemia of inflammation, and thus may be of considerable help in the differential diagnosis of microcytic anemia. This is particularly useful to identify concomitant iron deficiency in a patient with inflammation because ferritin values are then generally normal. Elevated sTfR levels are also the characteristic feature of functional iron deficiency, a situation defined by tissue iron deficiency despite adequate iron stores. The sTfR/ferritin ratio can thus describe iron availability over a wide range of iron stores. With the exception of chronic lymphocytic leukemia (CLL) and high-grade non-Hodgkin's lymphoma and possibly hepatocellular carcinoma, sTfR levels are not increased in patients with malignancies. We conclude that soluble TfR represents a valuable quantitative assay of marrow erythropoietic activity as well as a marker of tissue iron deficiency. D

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“…Several studies have demonstrated that TfR was expressed more abundantly in malignant tissues than in their healthy counterparts [2,[4][5][6][7][8] . The serum soluble transferrin receptor (sTfR) levels in the hepatoma and haematologic malignancies were significantly increased [9] . In light of these pieces of evidence, anti-TfR antibody (Ab) is regarded as an alternative for the localization diagnosis and targeting treatment of tumors [10][11][12][13][14][15][16] .…”

mentioning

confidence: 99%

Preparation and identification of scFv and bsFv against transferrin receptor

Liu

Xiao

Zhou

et al. 2008

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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To obtain single chain variable fragment (scFv) and bivalent single chain variable fragment (bsFv) against transferrin receptor, up-stream and down-stream primers were designed according to the complementary sequences of FR1 region of variable heavy (VH) and FR4 of variable light (VL), respectively, which contained inter-linker G4S and the restriction endonuclease SfiI, AscI and NotI. Two pieces of scFv fragments were first amplified through PCR and then inserted into plasmid pAB1, which could express scFv protein once induced by IPTG in the host bacteria. To express scFv and bsFv, E. coli TG1 was cultured in LB broth and was induced by IPTG. The restriction enzyme digestion map and DNA sequencing demonstrated that scFv and bsFv genes were successfully inserted into the expression plasmid. SDS-PAGE and Western blotting revealed the protein band at 35kD and 60kD, which were consistent with the molecular weight of scFv and bsFv respectively. Flow cytometry showed that scFv and bsFv harbored the specific binding activity with TfR expressed in various tumor cells, and the avidity of bsFv was higher than that of the parent scFv.

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Clinical Usefulness of Combined Measurements of Serum Soluble Transferrin Receptor Levels and Serum Interleukin-18 Levels at Determination of Serum KL-6 Levels in Haematologic Malignancies

Cited by 12 publications

References 12 publications

The significance of soluble transferrin receptors in diagnosing iron deficiency anemia

The significance of soluble transferrin receptors in diagnosing iron deficiency anemia

Soluble transferrin receptor for the evaluation of erythropoiesis and iron status

Preparation and identification of scFv and bsFv against transferrin receptor

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