Clinical usefulness of serum tartrate-resistant acid phosphatase in paget's disease of bone: Correlation with other biochemical markers of bone demodelling

Abstract

Tartrate resistant acid phosphatase (TRAP) has been proposed as a new biochemical marker for bone resorption. We have compared this new marker, TRAP, with the classical biochemical markers of bone remodelling, serum alkaline phosphatase (sAP), serum osteocalcin (sBGP), and with the urinary hydroxyproline/creatinine ratio (uOHProl/creatinine), a routine marker of bone resorption. Serum TRAP was significantly higher in pagetic patients (n = 43) than in control subjects (n = 12) (13.02 +/- 4.7 vs 5.48 +/- 1.31 IU/L, P less than 0.001) and a significantly positive linear correlation was found between the sTRAP and uOHProl/creatinine ratio (y = 0.0051x - 0.0069, r = 0.82, P less than 0.001), between sTRAP and sAP (y = 19.3x - 85.0, r = 0.71, P less than 0.001) and also between sTRAP and sBGP (y = 0.02x + 2.23, r = 0.52, P less than 0.01). Serum TRAP levels were higher than the upper limit of normality in all our pagetic patients except for two, whose uOHProl/creatinine levels were in the normal range. We conclude that (1) sTRAP could be a parameter as sensitive as uOHProl/creatinine in the diagnosis of Paget's disease; (2) sTRAP and uOHProl/creatinine are both good markers of bone resorption; (3) the correlation found between sTRAP and formation markers (sAP and sBGP) makes sTRAP a marker of disease activity in Paget's disease of bone; (4) the assay of sTRAP is easier, faster, and of lower cost than the urinary hydroxyproline determination. We suggest that sTRAP determination could be used as a routine marker of bone resorption in Paget's disease of bone, as is the case with uOHProl determination.

Acute effects of plyometric jumping and intermittent running on serum bone markers in young males

“…Values were shown as mean § SE; a the PL group signiWcantly diVerent from the CON group, P < 0.05 (Table 2). However, despite an inherent correlation between osteocalcin and TRAP (Malyszko et al 2003;Torres et al 1991), both speciWc bone metabolism markers did not correlate well to calcium and phosphorus. Similar results have been found in previous studies: blood calcium levels increased after exercise while TRAP concentration decreased (Ashizawa et al 1997), suggesting that the raised serum calcium after exercise was caused by short-term metabolic acidosis after exercise intervention, rather than by bone cellular activities.…”

Identification of inhibitors of Tartrate‐resistant acid phosphatase (TRAP/ACP5) activity by small‐molecule screening

“…TRAP has been implicated as a potential drug target because of its association with several pathologies, such as bone‐associated diseases, chronic inflammation, and cancer . Several attempts to find potent and specific inhibitors for TRAP have been performed; however, the compounds found are often toxic and lack drug‐like characteristics.…”

“…Aberrant appearance of either of the TRAP isoforms has been associated with different pathological conditions. For example, increased TRAP 5a expression has been reported in obesity, rheumatoid arthritis, and end‐stage renal disease, while the TRAP 5b isoform is associated with the development of bone diseases such as osteoporosis, Paget's disease of the bone, and multiple myeloma . In addition, several studies support the role of TRAP 5b as a serum marker for the detection of bone metastases .…”

Discriminative value of biochemical markers of bone turnover in assessing the activity of Paget's disease

“…Few studies have focused on the use of serum TRAP and ICTP in pagetic patients. (13314) Although a good correlation between serum TRAP and other markers of bone turnover in Paget's disease has been reported, (15) no mention of the usefulness of this marker in patients with low disease activity has been described. The development of an immunoassay using monoclonal antibodies specifically directed against the bone isoenzyme of serum TRAP could probably improve our poor results.…”