Clinical utility of a nested nucleic acid amplification format in comparison to viral culture for the diagnosis of mucosal herpes simplex infection in a genitourinary medicine setting

Coyle, Peter; O’Neill, Hugh J.; McCaughey, Conall; Wyatt, D.E.; McBride, M

doi:10.1186/1471-2334-1-11

Cited by 6 publications

(1 citation statement)

References 19 publications

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“…In particular, NATs can detect HSV with improved sensitivity in lesions that have progressed past the vesicular stage (12,17,52). Along with a very high sensitivity, NAT methods also have specificity consistently close to 100% (53)(54)(55)(56). In addition, NATs are much less affected by specimen storage beyond 48 h or by freezing, thawing, bacterial contamination and other factors that reduce virus viability (57,58).…”

Section: Natsmentioning

confidence: 99%

The Diagnosis of Genital Herpes – Beyond Culture: An Evidence‐Based Guide for the Utilization of Polymerase Chain Reaction and Herpes Simplex Virus Type‐Specific Serology

Ratnam

Severini

Zahariadis³

et al. 2007

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Accurate identification of persons with genital herpes is necessary for optimal patient management and prevention of transmission. Because of inherent inaccuracies, clinical diagnosis of genital herpes should be confirmed by laboratory testing for the causative agents herpes simplex virus type 1 (HSV-1) and HSV type 2 (HSV-2). Further identification of the HSV type is valuable for counselling on the natural history of infection and risk of transmission. Laboratory methods include antigen detection, culture, polymerase chain reaction (PCR) and conventional and type-specific serology (TSS). PCR has, by far, the greater sensitivity and should be the test of choice for symptomatic cases. HSV-2 TSS is indicated for patients with genital lesions in whom antigen detection, culture or PCR fail to detect HSV, and for patients who are asymptomatic but have a history suggestive of genital herpes. HSV-2 TSS is further indicated for patients infected with HIV. HSV-2 TSS along with HSV-1 TSS may be considered, as appropriate, in evaluating infection and/or immune status in couples discordant for genital herpes, women who develop their first clinical episode of genital herpes during pregnancy, asymptomatic pregnant women whose partners have a history of genital herpes or HIV infection, and women contemplating pregnancy or considering sexual partnership with those with a history of genital herpes. The above should be performed in conjunction with counselling of infected persons and their sex partners.

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Section: Natsmentioning

confidence: 99%

The Diagnosis of Genital Herpes – Beyond Culture: An Evidence‐Based Guide for the Utilization of Polymerase Chain Reaction and Herpes Simplex Virus Type‐Specific Serology

Ratnam

Severini

Zahariadis³

et al. 2007

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Real‐time nested multiplex PCR for the detection of Herpes simplex virus types 1 and 2 and Varicella zoster virus

O’Neill

Wyatt

Coyle

et al. 2003

Journal of Medical Virology

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One hundred forty-nine specimens were tested in a LightCycler nested multiplex polymerase chain reaction (LCnmPCR) for Herpes simplex virus (HSV)1, HSV2, and VZV. Eighty-one were from genitourinary medicine (GUM) patients and the other 68 specimens were from other patients with skin lesions. The results were compared to a conventional multiplex nested PCR (nmPCR) using agarose gel electrophoresis. Twenty-five specimens were positive in both assays for HSV1 and 29 were positive for VZV. For HSV2 there were 27 positive in the LCnmPCR and 26 positive in the nmPCR assay. The melting temperatures (Tms) of each target were different with a mean of 84.75 degrees C for HSV1, 88.57 degrees C for HSV2, and 83.62 degrees C for VZV. The melting curves of positive specimens directly overlaid the melting curves of the positive controls in the assay. The LCnmPCR assay is a convenient alternative to conventional PCR using agarose gel electrophoresis. It improves specimen turnaround time by eliminating the need for gel electrophoresis, transillumination, and gel photography. It also shows increased sensitivity for HSV2 over our standard assay. This LCnmPCR reduces further the possibility of amplicon contamination with nested PCR protocols.

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Herpes Diagnostic Tests and Their Use

Wagoner

Hook

2012

Curr Infect Dis Rep

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Clinical utility of a nested nucleic acid amplification format in comparison to viral culture for the diagnosis of mucosal herpes simplex infection in a genitourinary medicine setting

Abstract: Background: Nested nucleic acid amplification tests are often thought too sensitive or prone to generatingfalse positive results for routine use. The current study investigated the specificity and clinicalutility of a routine multiplex nested assay for mucosal herpetic infections.

Cited by 6 publications

References 19 publications

The Diagnosis of Genital Herpes – Beyond Culture: An Evidence‐Based Guide for the Utilization of Polymerase Chain Reaction and Herpes Simplex Virus Type‐Specific Serology

The Diagnosis of Genital Herpes – Beyond Culture: An Evidence‐Based Guide for the Utilization of Polymerase Chain Reaction and Herpes Simplex Virus Type‐Specific Serology

Real‐time nested multiplex PCR for the detection of Herpes simplex virus types 1 and 2 and Varicella zoster virus

Herpes Diagnostic Tests and Their Use

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