Clinical utility of a rapid test for uristatin

Pugia, Michael J.; Takemura, Toskihiho; Kuwajima, Shiro; Suzuki, Motoyohisa; Cast, Todd K; Profit, James A; Schulman, Lloyd S.; Ohta, Yoshihisa; Lott, John

doi:10.1016/s0009-9120(02)00288-6

Cited by 17 publications

(21 citation statements)

References 7 publications

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“…Sputum levels of the cytokine IL-8 are known to correlate with neutrophil counts in sputum [3,9], which in turn may correlate with NE levels in BAL [3]. Neutrophils are mobilised and the elevated UTI concentrations were observed in bacterial pneumonia [17,18]. In the current study, neither peripheral blood neutrophil counts nor CRP values showed a significant correlation with serum UTI concentrations.…”

Section: Discussioncontrasting

confidence: 62%

“…Several proteins are known to inactivate NE activity and NE degrades the pre-a-/inter-a-trypsin inhibitors, proteinase inhibitors, that release UTI [14,15]. UTI has been reported to be a positive acute phase protein whose concentration in serum and excretion in urine is increased in proportion to the invasiveness of the various diseases and the activation of neutrophils [17,18]. Previous study showed that both NE and UTI concentrations in BAL fluid of acute respiratory distress syndrome are significantly elevated, but only UTI concentration is significantly high in the serum [20].…”

Section: Discussionmentioning

confidence: 99%

“…Urinary trypsin inhibitor concentrations have already attracted attention as a useful index of the status of systemic inflammatory response syndrome [14,17,18]. It has been reported that neutrophil activation and cell infiltration develop in childhood asthmatic patients even in viral infections [7,9,11].…”

Section: Discussionmentioning

confidence: 99%

“…Assay of urinary trypsin inhibitor, neutrophil elastase, a 1 -antitrypsin, tissue inhibitor of metalloproteinase-1 and matrix metalloproteinase-2 Serum UTI was determined by sandwich-type one-step enzyme immunoassay using polyclonal antibodies to human trypsin inhibitor [14,17,18] and a human assay kit (Mochida Pharmaceutical Co. Ltd., Tokyo, Japan). The ELISA procedure makes it possible to specifically measure UTI by trapping pre-a-/inter-a-inhibitors in solid phase with a treatment of perchloric acid.…”

Section: Controlsmentioning

confidence: 99%

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Increased serum concentration of urinary trypsin inhibitor with asthma exacerbation

Yasui¹,

Kanda²,

Iwanami³

et al. 2003

Eur Respir J

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The aim of the study was to determine whether the amount of urinary trypsin inhibitor (UTI) in serum, a degenerate induced by neutrophil elastase (NE), reflects the degree of bronchial inflammation in children with acute asthma exacerbation.The involvement of neutrophil-mediated inflammation plays as important a role as eosinophil-mediated inflammation in the pathogenesis of acute asthma exacerbation. However, no measurable marker is sensitive enough to assess neutrophil-mediated inflammation in the airways. The pre-a-/inter-a-trypsin inhibitors are assumed to be precursors of UTI. NE degrades pre-a-/inter-a-trypsin inhibitors to liberate UTI. UTI concentrations in 25 childhood patients admitted with asthma exacerbation and 15 control subjects were measured by means of one-step sandwich-type enzyme immunoassay.Serum UTI concentrations in the patients at admission were significantly higher than control values (10.597¡0.649 and 6.136¡0.303 U?mL -1 , respectively (mean¡SEM)). These levels returned to baseline values with improvement in the asthmatic symptoms. However, serum NE and a 1 antitrypsin concentrations were not significantly different between patients and controls, even during acute exacerbation in the former.The findings suggest that neutrophil-mediated inflammatory events are involved in exacerbation of childhood asthma. The monitoring of urinary trypsin inhibitor concentrations might be useful for evaluating the neutrophil-mediated inflammation in childhood asthma attack. Eur Respir J 2003; 22: 739-742.

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Section: Discussioncontrasting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Controlsmentioning

confidence: 99%

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Increased serum concentration of urinary trypsin inhibitor with asthma exacerbation

Yasui¹,

Kanda²,

Iwanami³

et al. 2003

Eur Respir J

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“…The prevalent uTi forms include Bikunin (molecular weight=E30 kDa) and its Uristatin fragments, which lack the chondroitin sulfate chain. These fragments are generally o17 KDa, and all exhibit trypsin inhibitory activity (7,8).…”

Section: Introductionmentioning

confidence: 99%

Sensitive noninvasive marker for the diagnosis of probable bacterial or viral infection

Jortani

Pugia

Elin

et al. 2004

Clinical Laboratory Analysis

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Urinary trypsin inhibitor (uTi) is a product of elastase-mediated degradation of interleukin-alpha-inhibitor (I-alpha-I). Its activity increases in the urine of patients with a malignancy, inflammation, or infection, or in late pregnancy. The objective of this study was to compare the sensitivity of uTi in urine with that of serum quantitative C-reactive protein (CRP) for diagnosing infection, as indicated by white cell response and clinical assessment. Ninety controls and 171 patients with various systemic infections were enrolled. We measured uTi enzymatically on a Cobas Fara (Roche Diagnostics). Patients were separated into bacterial, probable bacterial, viral, or probable viral groups based on the results of a complete blood count with differential (CBC), urinalysis (UA), and clinical assessment. In the bacterial (n=70) and control (n=90) groups, the uTi values (mean+/-SE) were 25.3+/-3.1 mg/L and 2.8+/-0.8 mg/L, respectively. uTi (at 2.7 mg/L) had a diagnostic sensitivity of 91% and specificity of 82% (AUC=0.889), whereas CRP (at a cutoff of 10 mg/L) had a sensitivity and specificity of 82% and 96%, respectively (AUC=0.921). As a marker of infection (positive in both bacterial and viral groups), uTi had a sensitivity of 91% (AUC=0.884) vs. 89% (AUC=0.828) for CRP. Our data indicate that uTi has sufficient clinical sensitivity for screening systemic infections, and may have diagnostic value as a noninvasive test.

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Uristatin Anti-inflammatory Cellular Signaling

Basu

Pugia

2015

Inflammatory Pathways in Diabetes

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Clinical utility of a rapid test for uristatin

Cited by 17 publications

References 7 publications

Increased serum concentration of urinary trypsin inhibitor with asthma exacerbation

Increased serum concentration of urinary trypsin inhibitor with asthma exacerbation

Sensitive noninvasive marker for the diagnosis of probable bacterial or viral infection

Uristatin Anti-inflammatory Cellular Signaling

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