Clinical Utility of Assays for Circulating Immune Complexes

Endo, L.M.; Corman, Lourdes C.; Panush, Richard S.

doi:10.1016/s0025-7125(16)31011-2

Cited by 12 publications

(4 citation statements)

References 43 publications

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“…Certain properties influence their potential patho genicity. Features of particular importance in clude nature, size, and concentration of the antigen, nature, size, and concentration of the antibody (22), rate of forma tion and clearance of the ICs (23). Just where they end up they set off reactions that lead to inflammation and tissue damage.…”

Section: Discussionmentioning

confidence: 99%

Immune Complexes and Complement in Serum and Synovial Fluid of Rheumatoid Arthritis Patients

Mijusković¹,

Rackov²,

Pejović³

et al. 2009

Journal of Medical Biochemistry

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Immune Complexes and Complement in Serum and Synovial Fluid of Rheumatoid Arthritis PatientsRheumatoid arthritis (RA) is predominantly an intraarticular inflammatory and autoimmune disease that involves different autoantibodies and effector mechanisms. The aim of the study was to determine the utility of Circulating Immune Complexes (CIC) and complement components (C3c, C4) as possible markers for the disease activity in laboratory diagnostics. In a cross-section study 59 patients, according to the clinical criteria, were categorized into two groups: group with moderate (MA, n=24), and group with severe activity (SA, n=35) of RA. The concentration of CIC, C3c and C4 in sera (S) and synovial fluids (SF) was examined by an immunonephelometric method in both groups and compared with values in the control group (n=15) of patients with lesions of the menisci. Obtained results showed that there was no statistical significance in the values of C3c and C4, in both biological fluids, among all tested groups. Significant differences were found in the levels of CIC in both fluids, while testing the parameters (× ± SD, IU/mL) in the sera of groups with SA and MA of RA: 7.43 ± 13.40; 3.01 ± 2.92 (p<0.05) and SF: 13.47 ± 21.1, 5.33 ± 7.53 (p<0.001), respectively. These differences were higher between the group with SA and CG. Results for the concentrations of CIC were significantly higher in SF compared to sera: in the RA group with SA by 77% and group with MA by about 82%. These data could provide a confirmation of the hypothesis about local, intraarticular autoantibodies and subsequent CIC production. It can be concluded that the examination of CIC concentration in serum, and where it is possible in SF, is a useful marker of disease activity in RA patients, in contrast to the tested components of the complement. This statement does not exclude their consumption within immune effector mechanisms, but elicits the possibility that lower molecular fragments (C3d, C4d), as well as the novel activation products, could be better disease activity markers in RA patients.

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Section: Discussionmentioning

confidence: 99%

Immune Complexes and Complement in Serum and Synovial Fluid of Rheumatoid Arthritis Patients

Mijusković¹,

Rackov²,

Pejović³

et al. 2009

Journal of Medical Biochemistry

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“…The mononuclear phagocytes, erythrocytes, and complement systems, which are responsible for the removal of complexes, become overloaded and the complexes are trapped in the different tissues, e.g. kidneys, lung, skin, joints, or blood vessels where they may set off the complement reactions, causing inflammation of the small blood vessels (vasculitis) [1][2][3], eliciting different symptoms such as skin purpura and neuropathy, and leading ultimately to tissue damage. Deposition of IC in local tissues is common in rheumatic diseases, scleroderma, Sjögren's syndrome, and systemic lupus erythematosus.…”

Section: Introductionmentioning

confidence: 99%

“…Because of their clinical importance, IC have been determined by several methods [2][3][4][5], such as LC [6], precipitation with PEG [7][8][9][10][11][12], nephelometry [13][14]], and by different immunoassays [15]. These different methods can detect the whole antigen-antibody complex, the bound antigen, or fragments of the complement system.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of immune complexes by capillary electrophoresis

Shihabi

2008

Electrophoresis

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A simple method for immune complexes (IC) analysis by CE is described. This method combines the ease of precipitation of the IC by polyethylene glycol with the separation power of CE. The advantage of this method is a better quantitation of the IC, since it corrects and eliminates the interferences from other serum proteins. It also reveals the composition (monoclonality) of the precipitate. Three types of IC have been detected in this method: monoclonal, polyclonal and mixed (mono-polyclonal) IC. Furthermore, the method is rapid and simple.

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Uncontrolled trial of cryofiltration in rheumatoid arthritis

Malchesky

Smith

Koo

et al. 1988

J of Clinical Apheresis

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In an open trial of cryofiltration in rheumatoid arthritis at our institution, 19 patients received 464 treatments; the intensive schedule of ten treatments in 5 weeks was completed in 14 of the 19, and excellent to good clinical response was achieved in ten of these. The combined results demonstrated significant reductions in Ritchie index, morning stiffness, and 50-foot-walk time in the intensive treatment period, as well as significant increases in hand grip strengths. In the maintenance treatment period treatment frequency was individually selected and at less frequent intervals. Changes in clinical parameters were notably less. Although significant correlations were observed between the percent change in clinical parameters and the percent change in biochemical and hematological parameters, no consistent pattern was found. The favorable results of this trial and other uncontrolled trials has led to a two-center double-blind controlled trial of cryofiltration in rheumatoid arthritis that is now in progress.

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Clinical Utility of Assays for Circulating Immune Complexes

Cited by 12 publications

References 43 publications

Immune Complexes and Complement in Serum and Synovial Fluid of Rheumatoid Arthritis Patients

Immune Complexes and Complement in Serum and Synovial Fluid of Rheumatoid Arthritis Patients

Analysis of immune complexes by capillary electrophoresis

Uncontrolled trial of cryofiltration in rheumatoid arthritis

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