Clinical utility of loop‐mediated isothermal amplification assay for the diagnosis of common alpha herpesvirus skin infections

Kobayashi, Tatsuhiko; Yagami, Akiko; Suzuki, Kayoko; Ihira, Masaru; Yoshikawa, Tetsushi; Matsunaga, Kayoko

doi:10.1111/1346-8138.12325

Cited by 16 publications

(4 citation statements)

References 21 publications

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“…On the other hand, the sensitivity and specificity of the LAMP assay for VZV are similar to those of real-time PCR [ 11 ]. Clinical usefulness of the LAMP assay for the diagnosis of cutaneous herpesvirus infections have been reported [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Concurrent Reactivation of Herpes Simplex and Varicella Zoster Viruses Confirmed by the Loop-Mediated Isothermal Amplification Assay

Kobayashi¹,

Yagami²,

Suzuki

et al. 2014

Case Rep Dermatol

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Concurrent reactivation of herpes simplex and varicella zoster viruses is rare. Here, we describe the case of an elderly patient with herpes labialis and herpes zoster manifesting as a right-side facial eruption with vesicles and crusting. The loop-mediated isothermal amplification (LAMP) assay demonstrated the presence of both herpes simplex virus type 1 and varicella zoster virus in swab samples taken from the face, which was confirmed by real-time PCR, suggesting concurrent reactivation of both viruses. The use of the LAMP assay in the present case indicates its usefulness in the diagnosis of atypical herpes infections.

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Section: Introductionmentioning

confidence: 99%

Concurrent Reactivation of Herpes Simplex and Varicella Zoster Viruses Confirmed by the Loop-Mediated Isothermal Amplification Assay

Kobayashi¹,

Yagami²,

Suzuki

et al. 2014

Case Rep Dermatol

Self Cite

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“…Loop-Mediated Isothermal Amplification Technique: DNA amplification, with high efficiency and specificity, can also be achieved using the loop-mediated isothermal amplification (LAMP) method [ 28 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 ]. It involves the use of Bst DNA polymerase and a set of six primers that recognize specific sequences on the target DNA.…”

Section: Detection Of Hsv In Lesionsmentioning

confidence: 99%

Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

Nath

Kabir

Doust

et al. 2021

Infectious Disease Reports

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Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of physical contact. Additionally, viral shedding can also happen from asymptomatic infections. Thus, early and accurate detection of HSV is needed to prevent the transmission of this infection. Herpes can be diagnosed in two ways, by either detecting the presence of the virus in lesions or the antibodies in the blood. Different detection techniques are available based on both laboratory and point of care (POC) devices. Laboratory techniques include different biochemical assays, microscopy, and nucleic acid amplification. In contrast, POC techniques include microfluidics-based tests that enable on-spot testing. Here, we aim to review the different diagnostic techniques, both laboratory-based and POC, their limits of detection, sensitivity, and specificity, as well as their advantages and disadvantages.

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“…These methods of dilution or heating can save over thirty minutes of processing time, as with Nihonyanagi et al's heating protocol to release MRSA in CellEaseII (Biocosm Inc., Hyogo, Japan) diluents [169]. LAMP-based testing of swab samples can also be performed at room-temperature [192][193][194][195][196] or while heated [145,[197][198][199][200][201][202], as can MDA [84]. In particular, Mahony et al's LAMP-based test for influenza A and B achieved an analytical sensitivity of one genome equivalent, operating via a novel swab preparation procedure of vortexing and heating [198].…”

Section: Direct Naats For Oral Dermal and Conjunctival Swabsmentioning

confidence: 99%

Advances in Directly Amplifying Nucleic Acids from Complex Samples

Walker

Hsieh

2019

Biosensors

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Advances in nucleic acid amplification technologies have revolutionized diagnostics for systemic, inherited, and infectious diseases. Current assays and platforms, however, often require lengthy experimental procedures and multiple instruments to remove contaminants and inhibitors from clinically-relevant, complex samples. This requirement of sample preparation has been a bottleneck for using nucleic acid amplification tests (NAATs) at the point of care (POC), though advances in "lab-on-chip" platforms that integrate sample preparation and NAATs have made great strides in this space. Alternatively, direct NAATs-techniques that minimize or even bypass sample preparation-present promising strategies for developing POC diagnostic tools for analyzing real-world samples. In this review, we discuss the current status of direct NAATs. Specifically, we surveyed potential testing systems published from 1989 to 2017, and analyzed their performances in terms of robustness, sensitivity, clinical relevance, and suitability for POC diagnostics. We introduce bubble plots to facilitate our analysis, as bubble plots enable effective visualization of the performances of these direct NAATs. Through our review, we hope to initiate an in-depth examination of direct NAATs and their potential for realizing POC diagnostics, and ultimately transformative technologies that can further enhance healthcare.

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Clinical utility of loop‐mediated isothermal amplification assay for the diagnosis of common alpha herpesvirus skin infections

Cited by 16 publications

References 21 publications

Concurrent Reactivation of Herpes Simplex and Varicella Zoster Viruses Confirmed by the Loop-Mediated Isothermal Amplification Assay

Concurrent Reactivation of Herpes Simplex and Varicella Zoster Viruses Confirmed by the Loop-Mediated Isothermal Amplification Assay

Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

Advances in Directly Amplifying Nucleic Acids from Complex Samples

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