Clinical Value of Radioimmunoassay of Plasma Growth-Hormone-Releasing Factor

Saito, Haruhiko; Saito, Shiro; Yamazaki, Ryuichi; Hosoi, Eiji

doi:10.1016/s0140-6736(84)90565-8

Cited by 49 publications

(28 citation statements)

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“…The present finding of a lack of a temporal correlation between plasma GH and GHRH in the peripheral blood GHRH during the sleep test is distinct from the previous reports in adults by Saito et al [9] and in children by Ohyama et al [10]. One possible explanation for the difference may be due to the method for measuring plasma GHRH.…”

Section: Discussioncontrasting

confidence: 99%

“…Recent reports have indicated that the sleepassociated GH release is mediated by hypothalamic GHRH release [5,6]. Some previous reports suggested that the GHRH in the peripheral blood could reflect hypothalamic GHRH neuronal activities stimulated by 1-dopa [7], glucose [8] or sleep [9,10], although plasma GHRH concentrations were measured by RIAs combined with rather unreliable extraction methods [7,11].…”

mentioning

confidence: 99%

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Peripheral Plasma levels of Human Growth Hormone Releasing Hormone (GHRH) during the Sleep Test in Short Children

et al. 1998

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Section: Discussioncontrasting

confidence: 99%

mentioning

confidence: 99%

Peripheral Plasma levels of Human Growth Hormone Releasing Hormone (GHRH) during the Sleep Test in Short Children

et al. 1998

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“…In rats it has been shown that the mechanical ablation of GHRH neurones in the medial basal hypothalamus results in a 70% reduction of circulating ir-GHRH levels (34), indicating that the majority of the peripheral immunoreactivity could originate from hypothalamic sources. Changes in circulating ir-GHRH concentrations have been claimed to correlate with the secretion of GH (35,36), suggesting that peripheral plasma ir-GHRH measurements reflect hypothalamic function. Our results support the view that a considerable proportion of human peripheral ir-GHRH is derived from the hypothalamus, because the nocturnal ir-GHRH and GH peaks showed a close temporal relationship in most of the cases.…”

Section: Discussionmentioning

confidence: 99%

Nocturnal Release of Immunoreactive Growth Hormone-Releasing Hormone and Growth Hormone in Normal Children

et al. 1989

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ABSTRACT. To evaluate the role of growth hormonereleasing hormone (GHRH) in the physiologic release of growth hormone (GH) we studied the nocturnal secretion of immunoreactive GHRH (ir-GHRH) and its relationship to GH release and various stages of sleep in six prepubertal (three boys) and six pubertal children (two boys) with normal stature. Their ages ranged from 8.1 to 14.9 yr and their bone ages from 6.8 to 14.8 yr. Blood was withdrawn continuously between 2200-0600 h at a constant rate of 5 mL/20 min. The EEG was simultaneously registered. The ir-GHRH and GH data were analyzed by a discrete-pulse detection algorithm (Pulsar). The number of nocturnal ir-GHRH pulses varied from 0-8 (median 7) and the number of GH peaks from 2-6 (median 3). Pubertal children had significantly more ( p < 0.05) ir-GHRH pulses and the pulse amplitude was higher ( p < 0.05) than in the prepubertal children. There were no significant differences in the G H parameters between the two groups. The ir-GHRH peaks were not significantly related to any specific sleep stage. The majority of the GH pulses (71%) were associated with slow wave sleep ( p c: 0.001). Two-thirds (69%) of the GHRH peaks preceded closely or coincided with GH pulses ( p < 0.02). Pubertal subjects had more isolated ir-GHRH peaks than prepubertal children (p < 0.05). We conclude that the nocturnal secretion of ir-GHRH is pulsatile and, assuming that the peripheral plasma concentrations of ir-GHRH reflect its release from the hypothalamus, GHRH appears to play a physiologic role in the regulation of GH secretion. The partial dissociation between ir-GHRH and GH pulses suggests that other factors are also involved in the regulation of episodic GH release and/or that some of the circulating ir-GHRH originates from extrahypothalamic sources. Pubertal children have increased ir-GHRH secretion, the importance of which remains to be defined. specifically stimulates G H release in normal men (3-5) and it has been shown that increases in circulating concentrations of ir-GHRH are followed by GH release in some physiologic situations (6, 7). However, only a little is known about the role of GHRH in the generation of the nocturnal rhythm of GH secretion. Based on observations obtained by intravenous administration of GHRH in the rat it has been suggested that there is a steady-state GHRH and somatostatin release from the hypothalamus and an additional 3-to 4-h rhythmic surge of each peptide (8). This interplay provides for the integration of the ultradian rhythm of GH secretion, as observed in the peripheral circulation. Plotsky and Vale (9) have demonstrated with direct measurements of ir-GHRH and ir-somatostatin in the hypophysialportal circulation that ir-GHRH is secreted in a pulsatile fashion and that ir-GHRH pulses occur only in the presence of diminished somatostatin release. GHRH infusion studies in man also suggest that GH secretion is regulated by dynamic interaction between GHRH and somatostatin (10, 11). Our study was aimed at evaluating nocturnal variation in the circu...

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“…Plasma GH before and after a provocative test or a suppressive test was assayed by RIA [12]. Plasma GHRH was assayed as reported previously [14,15]. Data were presented as means ± SD with the number of samples shown as 'n'.…”

Section: Endocrine Studiesmentioning

confidence: 99%

Two Different Pituitary Adenomas in a Patient with Multiple Endocrine Neoplasia Type 1 Associated with Growth Hormone-Releasing Hormone-Producing Pancreatic Tumor: Clinical and Genetic Features.

et al. 1995

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Clinical Value of Radioimmunoassay of Plasma Growth-Hormone-Releasing Factor

Cited by 49 publications

References 0 publications

Peripheral Plasma levels of Human Growth Hormone Releasing Hormone (GHRH) during the Sleep Test in Short Children

Peripheral Plasma levels of Human Growth Hormone Releasing Hormone (GHRH) during the Sleep Test in Short Children

Nocturnal Release of Immunoreactive Growth Hormone-Releasing Hormone and Growth Hormone in Normal Children

Two Different Pituitary Adenomas in a Patient with Multiple Endocrine Neoplasia Type 1 Associated with Growth Hormone-Releasing Hormone-Producing Pancreatic Tumor: Clinical and Genetic Features.

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