Clinical, virological and phylogenetic characterization of a multiresistant HIV-1 strain outbreak in naive patients in southern Spain

Viciana, Isabel; González-Domenech, Carmen M.; Palacios, Rosario; Delgado, Marcial; Arco, Alfonso del; Téllez, Francisco; Jarilla, Francisco; Fernández, Sandra Revilla; Clavijo, Encarnación; Santos, Jesús

doi:10.1093/jac/dkv332

Cited by 7 publications

(7 citation statements)

References 23 publications

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“…Several studies have been reported in the literature about HIV genetic variability in PR and RT genes as indicators of drug resistance 13 – 15 , viral fitness 16 , and transmitted resistance 17 , 18 and as a mean to evaluate phylogenetic relationships among circulating virus 19 . However, these studies have been carried in HIV mono-infected (mi) populations.…”

Section: Introductionmentioning

confidence: 99%

HIV Reverse Transcriptase and Protease Genes Variability Can Be a Biomarker Associated with HIV and Hepatitis B or C Coinfection

Cantão

Almeida

Wolf

et al. 2018

Sci Rep

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Variability of the HIV reverse transcriptase (RT) and protease (PR) genes has been used as indicators of drug resistance and as a mean to evaluate phylogenetic relationships among circulating virus. However, these studies have been carried in HIV mono-infected populations. The goal of this study was to evaluate, for the first time, the HIV PR and RT sequences from HIV/HBV and HIV/HCV co-infected patients. HIV PR and RT genes were amplificated and sequenced to resistance analysis. The bioinformatics analysis was performed to infer about sequences clustering and molecular evolution. The results showed that the most frequent amino acid substitutions in RT were L214F (67.6%), I135T (55.9%), and in PR was V15I (41.2%). The molecular clock analysis showed that the HIV circulating in co-infected patients were separated in two clusters in the years 1999–2000. Some patients included as HIV mono-infected according patients’ medical records and inside the co-infected cluster were, in fact, co-infected by PCR analysis. Analysis of the decision trees showed susceptibility to lamivudine and emtricitabine were important attribute to characterize co-infected patients. In conclusion, the results obtained in this study suggest, for the first time, that HIV RT and PR genes variability could be a genetic biomarker to coinfection.

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Section: Introductionmentioning

confidence: 99%

HIV Reverse Transcriptase and Protease Genes Variability Can Be a Biomarker Associated with HIV and Hepatitis B or C Coinfection

Cantão

Almeida

Wolf

et al. 2018

Sci Rep

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“…While the definition of VF varied, many included 1 or 2 consecutive measurements of "detectable" HIV-1 RNA [31,32], or 1 or 2 consecutive measurements of HIV-1 RNA > or ≥50 copies/mL [33][34][35][36][37][38][39][40][41], >200 copies/mL [40,42,43], >400 copies/mL [37,44], ≥500 copies/mL [41], and/or >1000 copies/mL [45,46] as part of study-defined VF criteria. Many did not explicitly define VF criteria [47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62]. Real-world data by regimen are described in further detail in the following sections.…”

Section: Treatment-emergent Resistance By Regimenmentioning

confidence: 99%

A Comprehensive Literature Review of Treatment-Emergent Integrase Resistance with Dolutegravir-Based Regimens in Real-World Settings

Henegar,

Letang,

Wang

et al. 2023

Viruses

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After a decade of dolutegravir (DTG) use in various antiretroviral therapy combinations and in diverse populations globally, it is critical to identify HIV strains with reduced drug susceptibility and monitor emergent resistance in people living with HIV who experience virologic failure while on DTG-based regimens. We searched the PubMed, Embase, and Cochrane databases to identify studies that reported DTG resistance-associated mutations (RAMs) emerging under selection pressure. Our review showed that RAMs conferring resistance to DTG were rare in 2-drug and 3-drug regimens used in real-world cohorts, corroborating data from clinical trials. The potency of DTG in maintaining virologic suppression was demonstrated, even in cases of pre-existing resistance to companion drugs in the regimen. Estimates of DTG RAMs depended on the population and certain risk factors, including monotherapy, baseline resistance or lack of genotypic testing, treatment history and prior virologic failure, and suboptimal treatment adherence. The RAMs detected after virologic failure, often in heavily treatment-experienced individuals with prior exposure to integrase strand transfer inhibitors, were G118R, E138K, G140A/C/R/S, Q148H/K/R, N155H, and R263K. Overall, these data highlight the durable effectiveness and high barrier to resistance of DTG as part of combination antiretroviral therapy in a wide variety of settings.

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“…Importantly, molecular epidemiology analyses have been used to detect transmission clusters that had spread unnoticed to nonmolecular epidemiological analyses and tools [5][6][7]. Molecular epidemiology analyses have also been able to detect the expansion of drug-resistant HIV variants among the drug-naive population [8][9][10].…”

Section: Molecular Approaches To Study Hiv Epidemicsmentioning

confidence: 99%

Molecular Evolution Methods to Study HIV-1 Epidemics

Patiño-Galindo

Gónzález-Candelas

2018

Future Virol.

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Nucleotide sequences of HIV isolates are obtained routinely to evaluate the presence of resistance mutations to antiretroviral drugs. But, beyond their clinical use, these and other viral sequences include a wealth of information that can be used to better understand and characterize the epidemiology of HIV in relevant populations. In this review, we provide a brief overview of the main methods used to analyze HIV sequences, the data bases where reference sequences can be obtained, and some caveats about the possible applications for public health of these analyses, along with some considerations about their limitations and correct usage to derive robust and reliable conclusions.

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Clinical, virological and phylogenetic characterization of a multiresistant HIV-1 strain outbreak in naive patients in southern Spain

Abstract: We report a cluster of an HIV-1 strain with multiple resistance mutations that was transmitted over a period of >8 years, affecting 30 naive patients from the same geographical area. The strain was susceptible to first-line combination ART.

Cited by 7 publications

References 23 publications

HIV Reverse Transcriptase and Protease Genes Variability Can Be a Biomarker Associated with HIV and Hepatitis B or C Coinfection

HIV Reverse Transcriptase and Protease Genes Variability Can Be a Biomarker Associated with HIV and Hepatitis B or C Coinfection

A Comprehensive Literature Review of Treatment-Emergent Integrase Resistance with Dolutegravir-Based Regimens in Real-World Settings

Molecular Evolution Methods to Study HIV-1 Epidemics

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