Clinico-pathological responses and PCR detection of Corynebacterium pseudotuberculosis and its immunogenic mycolic acid extract in the vital organs of goats

Odhah, Mohammed Naji; Jesse, Faez Firdaus Abdullah; Chung, Eric Lim Teik; Mahmood, Zaid; Haron, Abd Wahid; Lila, Mohd Azmi Mohd; Zamri‐Saad, Mohd

doi:10.1016/j.micpath.2019.103628

Cited by 9 publications

(7 citation statements)

References 29 publications

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“…It is composed of alkyl-b-hydroxyl fatty acids of a specific length and complexity which represents a major lipid component of the cell wall of some bacteria including C. pseudotuberculosis and M. tuberculosis among others. The cell wall purified mycolic acid MAs has been shown to mimic certain vital features of natural infections with the actual bacteria as earlier reported (Korf et al, 2005;Odhah et al, 2019).…”

Section: Introductionmentioning

confidence: 63%

Immune Responses of Goats to Corynebacterium pseudotuberculosis and its Mycolic Acids (MAs) Extract

Odhah¹,

Jesse²,

Paul³

et al. 2023

JAHP

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Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis in small ruminants and is of considerable economic importance in many countries worldwide. The control of the disease in animals depends on the control of the disease in infected animals. Nevertheless, few countries where the disease was previously endemic have successfully eradicated the pathogen. This investigation was undertaken to determine the antibody titre (IgM and IgG) in does challenged with C. pseudotuberculosis and its Mycolic acids (MAs) immunogen extract. About 12 healthy crossbred female Boer goats were assigned into three groups (A, B and C), each comprising of 4 goats. Group A (Negative control group) was inoculated intradermally with 2 ml of sterile phosphate-buffered saline (PBS-pH 7); Group B (Mycolic acid group) was inoculated intradermally with 2 ml of immunogenic Mycolic acid extract (1g /ml); while group C (Positive control group) was inoculated intradermally with 2 ml of 10 9 colony-forming unit of live C. pseudotuberculosis. All the animals were observed for 90 days post-inoculation. Blood samples were collected via the jugular vein from all the groups before the inoculation and once weekly after the challenge until the end of the research period. The result of the study showed that the Immunoglobulin M (IgM) concentration in goats inoculated with C. pseudotuberculosis significantly (p<0.05) increased at week 2 (18.97±0.28 ng/ml), week 3 (33.97±0.59 ng/ml), week 4 (31.94±0.28 ng/ml) and week 5 (12.51±2.42). Whereas, in the mycolic acid group, the IgG antibody titre increased consistently from week 1 to week 3 (20.99±1.96 ng/ml; 15.52±3.78 ng/ml; 14.74±0.34 ng/ml) respectively. On the other hand, the Immunoglobulin G (IgG) concentration was found to significantly increase (p<0.05) after treatments and the increase persisted up till week 11 post-inoculation. The concentration of IgG steadily increased in C. pseudotuberculosis and MAs-treated groups, reaching the peak at week 9 (32.82±8.56 ng/ml) in C. pseudotuberculosis group and week 10 (28.41±1.27 ng/ml) in MAs group, then declined slowly from week 10 till week 12. In conclusion, there was an elevated level in the IgM and IgG antibodies post-infection with C. pseudotuberculosis and MAs. However, while the IgG antibody levels started declining at week 9 for C. pseudotuberculosis, the MAs group was sustained until week ten before reducing. Therefore, MAs can be used as an immunogen to control caseous lymphadenitis infection caused by C. pseudotuberculosis among small ruminants.

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Section: Introductionmentioning

confidence: 63%

Immune Responses of Goats to Corynebacterium pseudotuberculosis and its Mycolic Acids (MAs) Extract

Odhah¹,

Jesse²,

Paul³

et al. 2023

JAHP

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“…Survey 2 also underscored that thoracic and abdominal abscesses in adult sheep were associated with poor body condition scores. Similarly, body weight loss was linked to the CL-visceral form in infected goats [19,41,42]. This could be attributed to reduced appetite and compromised nutrient digestion and absorption [42].…”

Section: Discussionmentioning

confidence: 99%

Rearing Management and Its Impact on Caseous Lymphadenitis in Sheep

El Khalfaoui,

El Amiri,

Cabaraux

et al. 2024

Animals

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Two surveys were conducted to assess the prevalence and risk factors of ovine caseous lymphadenitis (CL) and explore the association between its superficial and visceral forms in Sardi sheep in Settat province, Morocco. A total of 1521 sheep, including 318 lambs < 6 months, 572 young sheep aged 6–24 months, and 631 adult sheep > 24 months, were examined from 57 flocks. Superficial CL prevalence levels were as follows: 69/318 in lambs, 292/572 in young sheep, and 114/631 in adult sheep. Additionally, 2124 sheep, including 1813 young and 311 adult sheep, were inspected in slaughterhouses ante- and post-mortem. Among all infected animals, the prevalence of visceral abscesses was notably higher in adult sheep (83%, OR = 9.8, 95% CI = 5.5–17.2) compared to young sheep (35%). Data on flock size, sex, age, body condition score, rearing methods, and hygienic measures were collected. Suspected CL cases were confirmed using quadruplex-PCR. Poor barn ventilation, lack of abscess control, and younger age were identified as key risk factors for CL. Additionally, a high percentage (89%) of animals with thoracic abscesses did not display superficial lesions. While age and abscess control have been reported in previous studies, barn ventilation appears to be a new, but important, risk factor. In conclusion, the identified risk factors in Settat province are linked to breeding management practices. Implementing regular abscess control measures and improving barn ventilation are essential for CL prevention.

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“…La infección primaria ocurre en el sitio de ingreso de la bacteria, con diseminación hematógena y linfática formando abscesos en los nódulos linfáticos más cercanos al sitio de infección (parotídeos, submandibulares, prefemorales, preescapulares, poplíteos o mamarios). Luego ocurre una infección secundaria con la formación de abscesos en ganglios linfáticos (torácicos, bronquiales y mediastínicos) y diversos órganos (63,64) . En ovinos la apariencia morfológica de los nódulos abscedados es la característica de capa de cebolla al presentar una distribución en láminas concéntricas fibrosas separadas por material caseoso.…”

Section: Patogénesis Y Mecanismos De Evasión Del Sistema Inmuneunclassified

Linfadenitis caseosa: factores de virulencia, patogénesis y vacunas. Revisión

Domínguez

Jiménez

Guerrero

2022

Rev. Mex. Cienc. Pecu.

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La linfadenitis caseosa es una enfermedad que afecta la producción ovina y caprina a nivel mundial. El agente etiológico es una bacteria Gram positiva, intracelular facultativa denominada Corynebacterium pseudotuberculosis biovar ovis. La enfermedad puede cursar con un desarrollo cutáneo o visceral, provocando deterioro en la condición física del animal, así como pérdidas en la producción de leche y carne, decomiso de las canales, rechazo de las pieles y como consecuencia, grandes pérdidas económicas. El estudio de los factores de virulencia y los mecanismos de patogénesis han permitido comprender esta enfermedad, así como establecer las moléculas diana para el desarrollo de nuevas vacunas. Existen vacunas comerciales disponibles a nivel mundial; sin embargo, la protección conferida por éstas no ha sido eficaz en el control de la enfermedad. Actualmente el uso de nuevas tecnologías ha permitido la obtención y caracterización de proteínas con potencial inmunogénico para el desarrollado de nuevas vacunas, las cuales podrían ser una alternativa para incrementar la protección. En el presente trabajo se exponen los principales factores de virulencia de Corynebacterium pseudotuberculosis, sus implicaciones en la patogénesis y las tendencias actuales en las formulaciones vacunales.

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Clinico-pathological responses and PCR detection of Corynebacterium pseudotuberculosis and its immunogenic mycolic acid extract in the vital organs of goats

Cited by 9 publications

References 29 publications

Immune Responses of Goats to Corynebacterium pseudotuberculosis and its Mycolic Acids (MAs) Extract

Immune Responses of Goats to Corynebacterium pseudotuberculosis and its Mycolic Acids (MAs) Extract

Rearing Management and Its Impact on Caseous Lymphadenitis in Sheep

Linfadenitis caseosa: factores de virulencia, patogénesis y vacunas. Revisión

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