Clinico-Pathological Studies of Marek's Disease in Chickens

Das, Supriya; Das, Debangshu Narayan; Panda, Sasmita; Sagarika, Sneha; Jena, Basanti

doi:10.5455/ijlr.20170720115734

Cited by 6 publications

(10 citation statements)

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“…Paleness of combs might be due to viral induced anaemia (Heidari et al, 2016) [7] . In addition there was marked reduction in egg production, reduction in size of eggs was observed in the present study was supported by Okonkwo (2015) [15] and Das et al (2018) [5] . In the present study few birds also revealed atrophied breast and thigh muscles, weakness of legs, sometimes paralysis with inward curvature of the toes and death of the birds in few days after showing the clinical signs was noticed.…”

Section: Biochemical Parameterssupporting

confidence: 88%

“…Our results are in line with the findings of earlier study by Payne and Rennie (1976) [17] in which they observed increase in total lymphocytes, eosinophils count and decrease in monocytes, heterophils and basophils count in the lymphoproliferative phase of the chickens affected with Marek's disease. Similarly Das et al (2018) [5] reported, significant increase in the TLC values and no significant difference between the mean values of haemoglobin concentration, total erythrocytes count, PCV, DLC among Marek's disease affected birds and the normal birds. Serum biochemical examination revealed significant increase in the triglycerides, AST levels and decrease in cholesterol levels and non significant decrease in albumin, ALT and phosphorus levels and increase in total protein, globulin, calcium levels in Marek's disease affected birds when compared to the control birds.…”

Section: Biochemical Parametersmentioning

confidence: 73%

“…(2017) [3] , Makwana et al (2018) [14] and Das et al (2018) [5] . Paleness of combs might be due to viral induced anaemia (Heidari et al, 2016) [7] .…”

Section: Biochemical Parametersmentioning

confidence: 99%

“…(2018) [5] in the chickens affected with Marek's disease. Significant increase in the lymphocytes count and non significant increase in the TLC, decrease in the heterophils and monocytes in the Marek's disease affected birds was noted in comparison to the normal birds.…”

Section: Biochemical Parametersmentioning

confidence: 99%

“…(2018) [5] and Kumar et al (2018) [12] . Immunocytochemical studies of impression smears taken from various organs like liver, heart, spleen, lungs, kidneys, ovaries, proventriculus having nodular growths from Marek's disease infected birds revealed immunopositive reaction in the cytoplasm of the lymphoid cells.…”

Section: Biochemical Parametersmentioning

confidence: 99%

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Clinico-pathological and Immunocytochemical studies on Marek’s Disease in poultry

Reddy¹,

Kumar²,

Amaravathi³

et al. 2021

Pharma Innovation

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The present investigation was carried out on layer flock belong to Livestock farm complex, College of Veterinary Science, Tirupati. Among 500 birds 41 birds revealed lesions suspected for Marek's disease and morphologically diagnosed on the basis of gross lesions, cytological smears and immunocytochemistry of different organs. Haematological and biochemical examination revealed significant increase in the lymphocytes count, AST, triglyceride levels and significant decrease in cholesterol levels in the Marek's disease affected birds when compared to normal birds. Clinically, birds affected with Marek's disease revealed emaciation, depression with drooping of the wings, anorexia, gasping, reduced weight gain, diarrhoea, pale combs, wattles with presence of nodules on combs, marked reduction in egg production and reduction in size of eggs. Few birds exhibited paralysis with inward curvature of the toes and death of the birds in few days after showing the clinical signs. Grossly, visceral organs (liver, heart, lungs, kidney, ovary, spleen, thymus, pancreas, proventriculus) were enlarged with presence of focal to discrete nodules. Cytological examination of the impression smears collected from the growths of the affected organs during necropsy revealed infiltration of pleomorphic lymphoid cells with thin rim of basophilic cytoplasm. Immunocytochemical staining of the smears revealed moderate to severe immunopositive reactivity for MDV antigens in the cytoplasm of the lymphoid cells.

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Section: Biochemical Parameterssupporting

confidence: 88%

Section: Biochemical Parametersmentioning

confidence: 73%

“…(2017) [3] , Makwana et al (2018) [14] and Das et al (2018) [5] . Paleness of combs might be due to viral induced anaemia (Heidari et al, 2016) [7] .…”

Section: Biochemical Parametersmentioning

confidence: 99%

Section: Biochemical Parametersmentioning

confidence: 99%

Section: Biochemical Parametersmentioning

confidence: 99%

See 3 more Smart Citations

Clinico-pathological and Immunocytochemical studies on Marek’s Disease in poultry

Reddy¹,

Kumar²,

Amaravathi³

et al. 2021

Pharma Innovation

View full text Add to dashboard Cite

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Investigation of a herpesvirus outbreak in mixed breeds of adult domestic ducks using next generation sequencing

et al. 2023

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This report characterizes the first lethal outbreak of Marek’s disease on a large farm of mixed-breed adult ducks (>18,000) and identifies the pathogen that resulted in high mortality (35%). Clinical signs included inappetence, respiratory distress, depression, muscle weakness, and ataxia. Post mortem revealed enlarged fragile liver mottled with miliary whitish spots and an enlarged spleen. Histopathology revealed hepatocellular necrosis with eosinophilic intra-nuclear inclusion bodies, necrosis of splenic follicles and degeneration/necrosis of renal tubules. The disease was tentatively diagnosed as a herpesvirus infection, confirmed by virus isolation from the liver. DNA was isolated from 15-year-old archival formalin-fixed tissues from infected ducks and subjected to next generation sequencing (NGS). Despite highly degraded DNA, short stretches of G- and C-rich repeats (TTAGGG and TAACCC) were identified as telomeric repeats frequently found in herpesviruses. Megablast and further investigative bioinformatics identified presence of Marek’s disease virus (MDV), a Gallid alphaherpesvirus type 2 (GAHV-2), as the cause of the acute fatal infection. The source of infection may be attributed to a dead migratory flamingo found close to the duck enclosures three days prior to the outbreak; hence, GAHV-2 may also be responsible for the fatal infection of the flamingo accentuated by heat stress. Considering the possible spread of this highly contagious and lethal virus from a flamingo to the ducks, and the increasing zoonosis of animal viruses into humans, such as monkey B alphaherpesvirus transmission from macaques to humans with ~80% fatality, this observation has important ramifications for human health and safety of the poultry industry.

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Economic and value chain analysis to support an investigation and risk mitigation efforts on Marek's disease in layers in the southern part of Thailand

et al. 2023

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Background and Aim: Marek's disease (MD) is a common lymphoproliferative disease affecting chickens and causing economic losses in commercial poultry. The MD outbreak was noticed in the southern part of Thailand in 2019. The suspected cases were found with an abnormal number of cases of layers dying with clinical signs, for example, weakness and emaciation, with evidence of MD gross lesions. This study aimed to raise awareness of the MD outbreak through value chain analysis (VCA), identifying associated possible risk factors, and estimating the associated economic impact. Materials and Methods: Value chain analysis, including seasonal calendar, value chain diagram, and layer movement mapping of the layer industry, was conducted. High-risk stakeholders were identified on the basis of risk practices and interactions between stakeholders. A case–control study was conducted to determine risk factors associated with the MD outbreak on layer farms, and partial budget analysis was used to estimate economic losses associated with MD. Results: The value chain diagram showed the linkages between stakeholders, including estimation of the percentage of products moved from one stakeholder group to another and the negotiated price. Fourteen out of 35 layer farms were case farms. Farm size and source of birds were significantly associated with the MD outbreak. The MD outbreak caused total economic losses of 295,823 USD. Farms that slaughtered infected birds with additional revenues incurred losses of 140,930 USD, whereas farms that culled infected birds without additional revenue returned incurred losses of 1995 USD. Conclusion: The VCA provided a better understanding of the layer and egg businesses in South Thailand and guided the development of questionnaires for outbreak investigation. The potential risk factor findings suggested the need for further exploration of the source of the MD outbreak.

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Clinico-Pathological Studies of Marek's Disease in Chickens

Abstract: The present study was aimed to investigate/ study the pathology of Marek's disease in chickens by

Cited by 6 publications

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Clinico-pathological and Immunocytochemical studies on Marek’s Disease in poultry

Clinico-pathological and Immunocytochemical studies on Marek’s Disease in poultry

Investigation of a herpesvirus outbreak in mixed breeds of adult domestic ducks using next generation sequencing

Economic and value chain analysis to support an investigation and risk mitigation efforts on Marek's disease in layers in the southern part of Thailand

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