Clinicobiochemical investigations of gangrenous mastitis in does: immunological responses and oxidative stress biomarkers

Abstract

Abstract:A total of 50 does were used to determine selected hematological and biochemical parameters with special references to oxidative stress markers, acute phase protein profiles, and proinflammatory cytokines in healthy and gangrenous mastitis affected does. Animals were divided into two equal groups represented as clinically healthy (control) and diseased groups, respectively. The bacteriological examination of milk samples from diseased does revealed many types of bacterial infection. The isolated bacteria were Staphylococcus aureus (N=23/25), Escherichia coli (N=11/25), and Clostridium perfringens (N=4/25). There was a significant increase in the levels of β-hydroxybutyrate, non-esterified free fatty acids, triglyceride, low density lipoprotein cholesterol (LDL-C), aspartate aminotransferase, and alanine aminotransferase and a significant reduction in the levels of glucose, cholesterol, and high density lipoprotein cholesterol (HDL-C) in does with gangrenous mastitis compared to healthy does. Moreover, there was a significant increase in the levels of malondialdehyde and uric acid with a significant decrease in the levels of reduced glutathione, super oxide dismutase, and catalase in does with gangrenous mastitis compared to healthy does. In addition, there was a significant increase in the haptoglobin, serum amyloid A, fibrinogen, interleukin 6 (IL-6), IL-1β, and tumor necrosis factor-α (TNF-α) in does with gangrenous mastitis compared to healthy ones. Conclusively, oxidative stress biomarkers, acute phase proteins, and proinflammatory cytokines play an essential task as biomarkers for gangrenous mastitis in does. Mastitis may be considered as one of the ketotic stressors in does after parturition.

Acute phase response and oxidative stress parameters in pneumonic camel calves (Camelus dromedarius)

“…They reported that oxidative stress may induce lipid peroxidation and severe tissue lesions which result in damaged cell organelles, nucleic acids, lipids and proteins. The present findings of oxidative stress are also in harmony with Art et al (1999) and Deaton et al (2004) in horses with recurrent airway obstruction, Ledwozyw & Stolarczyk (1992) in calves with bronchopneumonia, Al-Qudah (2009) in calves with acute pneumonia and in does with gangrenous mastitis (El-Deeb, 2013).…”

Relationship between cortisol and acute phase protein concentrations in female rabbits

“…Despite the potential value of APPs as stress biomarkers, there are few published studies in rabbits that analyse the relationships between APPs and health status (Sun et al, 2005;Dishlyanova et al, 2011;El-Deeb, 2013). Our hypothesis was that lactation would be a stressful period for female rabbits and that cortisol and APP concentrations in blood would increase.…”

A variety of gene polymorphisms associated with idiopathic granulomatous mastitis

“…IGM, is a rare inflammatory breast disease although it is mostly accepted as an autoimmune disease [ 2 ]. In various publications, MTHFR-homozygous 677 TTR mutations [ 6 ], PAI-1 5 G/5 G polymorphism [ 7 ], β-fibrinogen heterozygous 455 G>A mutation [ 8 ], ACE I/D mutation [ 9 ] are reported as closely related with inflammatory, autoimmune events and breast cancer. In the studies, there seem to be a genetic predisposition in the development of many autoimmune and malignant diseases [ 10 ].…”