Clinicohistopathological implications of MMP/VEGF expression in retinoblastoma: a combined meta-analysis and bioinformatics analysis

Zhu, Jianwei; Zhang, Xi; Ai, Liqianyu; Yuan, Rongdi; Ye, Jian

doi:10.1186/s12967-019-1975-3

Cited by 16 publications

(14 citation statements)

References 56 publications

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“…MMPs are major proteolytic enzymes involved in tumour cell metastasis [47]. The overexpression of MMPs and VEGF can lead to malignant behaviour and are correlated with poor prognosis in many tumours [51][52][53][54]. Accordingly, in our study, we also found that silencing VEGF in NPC cells led to a decrease in N-cadherin, vimentin and MMPs and increase in E-cadherin, suggesting that VEGF can affect metastasis via EMT and MMPs in NPC cells.…”

Section: Discussionsupporting

confidence: 75%

VEGF promotes migration and invasion by regulating EMT and MMPs in nasopharyngeal carcinoma

et al. 2020

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Background: Vascular endothelial growth factor (VEGF) is an important pro-angiogenic factor. Accumulating data have indicated that VEGF is involved in tumour metastasis. However, the mechanism through which VEGF regulates nasopharyngeal carcinoma (NPC) metastasis is largely unknown. This study aimed to examine the biological function of VEGF in NPC metastasis and its underlying mechanism. Methods: We used western blotting and qPCR to examine the difference in VEGF expression between NPC cells and the immortalized nasopharyngeal epithelial cell line NP69. Wound healing assays, transwell assays and animal experiments were used to further verify the role of VEGF in the invasion and migration of NPC cells. The protein levels of the epithelial-mesenchymal transition (EMT) and matrix metalloproteinase (MMP) family were analysed by immunofluorescence (IF) and western blotting. Enzyme-linked immunosorbent assay (ELISA) and transwell assays were used to determine whether VEGF enhanced the invasion and migration of NPC cells in an autocrine manner. Western blotting was used to examine how autocrine VEGF-VEGFR2 signalling regulated EMT and MMPs. Results: We observed higher levels of VEGF in NPC cells than that in NP69 cells and identified an association between high VEGF levels and tumour invasion and migration. Mechanistically, the VEGF-mediated increase in EMT markers, MMP2 and MMP9 promoted NPC cell invasion and migration. Additionally, NPC cells secreted VEGF to promote cell invasion, migration and angiogenesis. Autocrine VEGF-VEGFR2 signalling increased ERK1/2 phosphorylation, promoted EMT process and MMPs at the indicated times. Conclusion: This study revealed that VEGF plays a role in controlling NPC cell metastasis by regulating EMT markers and MMPs in an autocrine manner.

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Section: Discussionsupporting

confidence: 75%

VEGF promotes migration and invasion by regulating EMT and MMPs in nasopharyngeal carcinoma

et al. 2020

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“…Previous studies demonstrated that MMP2/9 are important prognostic factors for various cancers. MMP2 and MMP9 overexpression was associated with poor prognosis in oral cancers [ 12 ], retinoblastoma [ 13 ], bladder cancer [ 14 ], and ovarian epithelial cancer [ 15 ]. The prognostic value of MMP2/9 in BC has also been investigated.…”

Section: Introductionmentioning

confidence: 99%

Prognostic values of tumoral MMP2 and MMP9 overexpression in breast cancer: a systematic review and meta-analysis

Jiang

2021

BMC Cancer

142

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Background Breast cancer (BC) is a leading cause of cancer-related death in females worldwide. Previous studies have demonstrated that matrix metalloproteinases (MMPs) play key roles in metastasis and are associated with survival in various cancers. The prognostic values of MMP2 and MMP9 expression in BC have been investigated, but the results remain controversial. Thus, we performed the present meta-analysis to investigate the associations between MMP2/9 expressions in tumor cells with clinicopathologic features and survival outcome in BC patients. Methods Eligible studies were searched in PubMed, Web of Science, EMBASE, CNKI and Wanfang databases. The associations of MMP2/9 overexpression in tumor cells with overall survival (OS), disease-free survival (DFS) and recurrence-free survival (RFS) were assessed by hazard ratio (HR) and 95% confidence interval (CI). The associations of MMP2/9 overexpression with clinicopathological features were investigated by calculating odds ratio (OR) and 95% CI. Subgroup analysis, sensitivity analysis, meta-regression, and analysis for publication bias were performed. Results A total of 41 studies comprising 6517 patients with primary BC were finally included. MMP2 overexpression was associated with an unfavorable OS (HR = 1.60, 95% CI 1.33 –1.94, P < 0.001) while MMP9 overexpression predicted a shorter OS (HR = 1.52, 95% CI 1.30 –1.77, P < 0.001). MMP2 overexpression conferred a higher risk to distant metastasis (OR = 2.69, 95% CI 1.35–5.39, P = 0.005) and MMP9 overexpression correlated with lymph node metastasis (OR = 2.90, 95% CI 1.86 – 4.53, P < 0.001). Moreover, MMP2 and MMP9 overexpression were both associated with higher clinical stage and histological grade in BC patients. MMP9 overexpression was more frequent in patients with larger tumor sizes. Conclusions Tumoral MMP2 and MMP9 are promising markers for predicting the prognosis in patients with BC.

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“…MMPs are a group of zinc endopeptidases vital for the breakdown of ECM components and basement membrane. Studies have demonstrated that MMP9 is a key prognostic factor for various cancers such as retinoblastoma, bladder cancer, and ovarian epithelial cancer [7][8][9]. In a meta-analysis done by Wei Deng and his colleagues on overexpression of MMPs in Oral cancer patients, have included 15 studies evaluating different types of MMPs concerning 1266 patients.…”

Section: Discussionmentioning

confidence: 99%

Role of Matrix Metalloproteinase 9 in Predicting Lymph Node Metastases in Oral Squamous Cell Carcinoma

Chakraborty¹,

Suresh²,

Mohiyuddin³

2023

Cureus

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BackgroundOral cancer is a common malignancy worldwide, with approximately 3,50,000 new cases diagnosed yearly. Out of many factors which affect the survival in patients with oral cancer, lymph node metastases are a major factor that reduces survival by 50%. Even though many biomarkers have been studied to predict lymph node metastasis, none have yet been accepted for routine use. Matrix metalloproteinases (MMPs) play a vital role in extracellular matrix (ECM) degradation, thus facilitating the invasive potential and the metastatic cascade of tumors. Of the different subtypes, multiple studies have demonstrated that matrix metalloproteinase 9 (MMP9) overexpression is often associated with the aggressive nature of the tumor. Therefore, this investigation is done to know the role of MMP9 in predicting lymph node metastasis in oral squamous cell carcinoma (OSCC). AimTo determine the immunohistochemical expression of MMP9 in OSCC and to find its association with lymph node metastasis. Settings and designIt is a laboratory-based observational study. Materials and methodsOne hundred five histologically proven cases of OSCC were studied. Histopathological parameters like depth of invasion, presence of lymph node metastasis, grading, and TNM staging were done according to the 8th AJCC staging criteria. Both intensity and proportion of MMP9 expression were recorded. Statistical analysisFor qualitative data, the Chi-square test was used as a test of significance. The p-value (probability that the result is true) of <0.05 was considered statistically significant after assuming all the rules of statistical tests. ResultsA higher expression of MMP9 was observed in 56.2% of cases and the higher expression correlated with the presence of lymph node metastases (p<0.001), an advanced stage of cancer (P <0.001), and grade of the tumor (p=0.003). ConclusionA positive association between MMP9 and lymph node metastasis and pathological TNM staging demonstrates MMP9 as a potential biomarker to predict the behavior of the tumor.

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Clinicohistopathological implications of MMP/VEGF expression in retinoblastoma: a combined meta-analysis and bioinformatics analysis

Cited by 16 publications

References 56 publications

VEGF promotes migration and invasion by regulating EMT and MMPs in nasopharyngeal carcinoma

VEGF promotes migration and invasion by regulating EMT and MMPs in nasopharyngeal carcinoma

Prognostic values of tumoral MMP2 and MMP9 overexpression in breast cancer: a systematic review and meta-analysis

Role of Matrix Metalloproteinase 9 in Predicting Lymph Node Metastases in Oral Squamous Cell Carcinoma

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