Clinicopathologic Characteristics and Prognoses for Multicentric Occurrence and Intrahepatic Metastasis in Synchronous Multinodular Hepatocellular Carcinoma Patients

Li, Shi-Lai; Su, Ming; Peng, Tao; Xiao, Kaiyin; Shang, Liming; Xu, Banghao; Su, Zhiying; Ye, Xinping; Peng, Ning; Qin, Quanlin; Chen, Defeng; Chen, Jie; Li, Le‐Qun

doi:10.7314/apjcp.2013.14.1.217

Cited by 25 publications

(29 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hepatitis and cirrhosis are a host factor and known to be the risk factors of intrahepatic recurrence related to multicentric occurrence [5,24]. The present study shows that hepatitis B, hepatitis C, porto-periportal activity grade, and stage of cirrhosis are not related to ER.…”

Section: Discussionmentioning

confidence: 47%

See 1 more Smart Citation

The risk factors of early recurrence after hepatectomy in hepatocellular carcinoma

et al. 2014

View full text Add to dashboard Cite

PurposeEarly recurrence after hepatectomy is a well-known poor prognostic factor in patients with hepatocellular carcinoma. This study was undertaken to identify the risk factors of early recurrence in patients with hepatocellular carcinoma after hepatectomy.MethodsOne hundred and sixty-seven patients that underwent hepatectomy for hepatocellular carcinoma from January 2005 to December 2010 were enrolled. The numbers of patients with or without early recurrence group were 40 and 127, respectively. Clinico-pathologic factors were retrospectively analyzed.ResultsPotential risk factors were classified as host, tumor, or surgical factors. Of the host factors examined, lobular hepatitis activity was found to be a significant risk factor of early recurrence, and of the tumor factors, infiltrative type of gross appearance, level of preoperative AFP and worst Edmondson-Steiner grade were significant.ConclusionThe present study shows that an infiltrative gross appearance, a high preoperative AFP level, high lobular hepatitis activity, and a poor Edmondson-Steiner grade are independent risk factors of early recurrence. Accordingly, patients with these risk factors should be followed closely after hepatectomy.

show abstract

Section: Discussionmentioning

confidence: 47%

“…Histological grade (E-S) and its relation with ER are different depending on each reporter [23,24,25]. In this study, poor E-S grade acted as the important risk factor of ER.…”

Section: Discussionmentioning

confidence: 83%

The risk factors of early recurrence after hepatectomy in hepatocellular carcinoma

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The detection of specific mutations in individual cancer populations has recently become a popular area of research. In a previous study conducted by the authors, it was observed that analysing sequence variations in the mtDNA D-loop region may determine useful biomarkers for identifying the cell clonal origin of synchronous multinodular HCC (13). In the current study, HCC tissue samples were screened for mtDNA polymorphisms and mutations, and their association with HCC prognoses were analysed.…”

Section: Discussionmentioning

confidence: 94%

“…The mtDNA fragments were amplified using the following primers: Forward, 5'-ATT CTA ACC TGA ATC GGA GG-3'; and reverse, 5'-GAT GCT TGC ATG TGT AAT CT-3' (Sangon Biotech Co., Ltd., Shanghai, China). The fragments were 1, 528 bp in length, including the complete D-loop region of 1,122 bp, as described previously (13). Each DNA sample (50 ng) was amplified using polymerase chain reaction (PCR; MJ Research PTC-200 Thermal Cycler; Bio-Rad Laboratories, Inc., Hercules, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…The direct sequencing primers were the same as those used for amplification. As there were numerous structures containing poly-C in the mtDNA D-loop region, the following two additional primers were used for PCR experiments (F2: 5'-AAT AC TTG ACC ACC TGT AG-'3; and F3: 5-'CCT ATG TCG CAG TAT CTG TC-'3) to sequence the entire mtDNA D-loop region (13). Multiple primers overlapped the sequenced regions.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Associations between sequence variations in the mitochondrial DNA D-loop region and outcome of hepatocellular carcinoma

Wan

Peng

et al. 2016

Oncology Letters

Self Cite

View full text Add to dashboard Cite

Abstract. The association between mitochondrial DNA (mtDNA) polymorphisms or mutations and the prognoses of cancer have been investigated previously, but the results have been ambiguous. In the present study, the associations between sequence variations in the mtDNA D-loop region and the outcomes of patients with hepatocellular carcinoma (HCC) were analysed. A total of 140 patients with HCC (123 males and 17 females), who were hospitalised to undergo radical resection, were studied. Polymerase chain reaction and direct sequencing were performed to detect the sequence variations in the mtDNA D-loop region. Multivariate and univariate analyses were conducted to determine important factors in the prognosis of HCC. A total of 150 point sequence variations were observed in the 140 cases (13 point mutations, 8 insertions, 20 deletions and 116 polymorphisms). The variation rate was 13.4% (150/1, 122). mtDNA nucleotide 150 (C/T) was an independent factor in the logistic regression for early/late recurrence of HCC. Patients with 150T appeared to have later recurrences. In a Cox proportional hazards regression model, hepatitis B virus DNA, Child-Pugh class, differentiation degree, tumour-node-metastasis (TNM) stage, nucleotide 16263 (T/C) and nucleotide 315 (N/insertion C) were independent factors for tumour-free survival time. Patients with the 16263T allele had a greater tumour-free survival time than patients with the 16263C allele. Similarly, patients with 315 insertion C had a superior tumour-free survival time when compared with patients with 315 N (normal). In the Cox proportional hazards regression model, recurrence type (early/late), Child-Pugh class, TNM stage and adjuvant treatment after tumour recurrence (none or one/more than one treatment) were independent factors for overall survival. None of the mtDNA variations served as independent factors. Patients with late recurrence, Child-Pugh class A, and low TNM stages and/or those who received more than one adjuvant treatment following tumour recurrence had favourable outcomes. mtDNA D-loop polymorphisms were associated with early recurrence and tumour-free survival time, but not with overall survival. mtDNA D-loop mutations in HCC were infrequent and lacked prognostic utility. The detection of mtDNA D-loop polymorphisms may assist in identifying risk factors for HCC prognosis, particularly for the short-term outcome, thereby aiding the construction of an appropriate therapeutic strategy.

show abstract

PDSS2‐Del2, a new variant of PDSS2, promotes tumor cell metastasis and angiogenesis in hepatocellular carcinoma via activating NF‐κB

et al. 2020

View full text Add to dashboard Cite

Hepatocellular carcinoma (HCC) is among the leading causes of cancer-related mortality worldwide. Our previous study identified a novel alternative splicing variant of prenyl diphosphate synthase subunit 2 (PDSS2) in HCC characterized by a deletion of exon 2, named PDSS2-Del2, which is devoid of the tumor-suppressive function of full-length PDSS2 (PDSS2-FL). To better understand the clinical significance of PDSS2-Del2, we performed a BaseScope assay on an HCC tissue microarray and found that positive staining for PDSS2-Del2 predicted a worse overall survival in patients with HCC (P=0.02). PDSS2-Del2 levels correlated significantly with microvessel counts in HCC tumor tissues. Importantly, PDSS2-Del2 overexpression functionally promoted HCC metastasis as demonstrated by in vitro and in vivo migration assays. In vivo assays also demonstrated that PDSS2-Del2 increased angiogenesis in xenografts. Furthermore, we discovered that elevated PDSS2-Del2 expression in HCC tumor cells decreased fumarate levels and activated canonical NF-κB pathway. The epithelial-to-mesenchymal transition (EMT) and WNT/β-catenin signaling pathways were also activated by overexpression. Dimethyl fumarate (DMF), a fumaric acid ester, effectively reduced the metastasis induced by PDSS2-Del2 as observed with in vivo spleen-liver metastasis animal experiments. Considering that DMF is a prescribed oral therapy for multiple sclerosis, it might be a potential treatment for metastasis of patients with HCC. Early clinical trials are needed to validate its potential in this context.

show abstract

Clinicopathologic Characteristics and Prognoses for Multicentric Occurrence and Intrahepatic Metastasis in Synchronous Multinodular Hepatocellular Carcinoma Patients

Cited by 25 publications

References 24 publications

The risk factors of early recurrence after hepatectomy in hepatocellular carcinoma

The risk factors of early recurrence after hepatectomy in hepatocellular carcinoma

Associations between sequence variations in the mitochondrial DNA D-loop region and outcome of hepatocellular carcinoma

PDSS2‐Del2, a new variant of PDSS2, promotes tumor cell metastasis and angiogenesis in hepatocellular carcinoma via activating NF‐κB

Contact Info

Product

Resources

About