Clinicopathological and prognostic significance of circulating tumor cells in patients with lung cancer: a meta-analysis

Xu, Tongwen; Shen, Guodong; Cheng, Min; Wang, Xu; Shen, Gan; Hu, Shilian

doi:10.18632/oncotarget.19122

Cited by 7 publications

(5 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Identification of circulating molecular biomarkers is therefore critical to improve early detection of lung cancer and represents an important approach with a large clinical potential [ 3 ]. Despite the recent improvements in the characterization of different circulating biomarkers such as cell free DNA [ 4 , 5 ], circulating tumor cells [ 6 – 8 ], extracellular vesicles or circulating miRNA [ 9 , 10 ], the search for optimal biomarkers still remains a challenge. In the past, most studies were centered on identifying potential biomarkers using molecules differentially expressed by tumor cells.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic role of circulating extracellular matrix-related proteins in non-small cell lung cancer

et al. 2018

View full text Add to dashboard Cite

BackgroundInteractions between cancer cells and the surrounding microenvironment are crucial determinants of cancer progression. During this process, bi-directional communication among tumor cells and cancer associated fibroblasts (CAF) regulate extracellular matrix (ECM) deposition and remodeling. As a result of this dynamic process, soluble ECM proteins can be released into the bloodstream and may represent novel circulating biomarkers useful for cancer diagnosis. The aim of the present study was to measure the levels of three circulating ECM related proteins (COL11A1, COL10A1 and SPARC) in plasma samples of lung cancer patients and in healthy heavy-smokers controls and test whether such measurements have diagnostic or prognostic value.MethodsGene expression profiling of lung fibroblasts isolated from paired normal and cancer tissue of NSCLC patients was performed by gene expression microarrays. The prioritization of the candidates for the study of circulating proteins in plasma was based on the most differentially expressed genes in cancer associated fibroblasts. Soluble ECM proteins were assessed by western blot in the conditioned medium of lung fibroblasts and by ELISA assays in plasma samples.ResultsPlasma samples from lung cancer patients and healthy heavy-smokers controls were tested for levels of COL11A1 and COL10A1 (n = 57 each) and SPARC (n = 90 each). Higher plasma levels of COL10A1 were detected in patients (p ≤ 0.001), a difference that was driven specifically by females (p < 0.001). No difference in COL11A1 levels between patients and controls was found. SPARC levels were also higher in plasma patients than controls (p < 0.001) with good performance in discriminating the two groups (AUC = 0.744). No significant association was observed between plasma proteins levels and clinicopathological features or survival.ConclusionSoluble factors related to proficient tumor-stroma cross-talk are detectable in plasma of primary lung cancer patients and may represent a valuable complementary diagnostic tool to discriminate lung cancer patients from healthy heavy-smokers individuals as shown for the SPARC protein.Electronic supplementary materialThe online version of this article (10.1186/s12885-018-4772-0) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 99%

Diagnostic role of circulating extracellular matrix-related proteins in non-small cell lung cancer

et al. 2018

View full text Add to dashboard Cite

show abstract

“…At each time point (first, second, and third quartiles of RFS), PB-CTCs showed excellent predictive value and the highest AUC for the third quartile of RFS compared to PV- and BM-CTCs. A meta-analysis showed that CTCs were significantly correlated with overall survival and progression-free survival in lung cancer patients [ 17 ]. Although many studies have shown similar results, the first point of note in our study is that it analyzed surgically resected lung cancer patients, and the second was that the analyzed samples were not only limited to PB, but also included samples from the PV and BM, unlike similar previous studies.…”

Section: Discussionmentioning

confidence: 99%

Prognostic Role of Circulating Tumor Cells in the Pulmonary Vein, Peripheral Blood, and Bone Marrow in Resectable Non-Small Cell Lung Cancer

et al. 2022

View full text Add to dashboard Cite

Background Studies of the prognostic role of circulating tumor cells (CTCs) in early-stage non-small cell lung cancer (NSCLC) are still limited. This study investigated the prognostic power of CTCs from the pulmonary vein (PV), peripheral blood (PB), and bone marrow (BM) for postoperative recurrence in patients who underwent curative resection for NSCLC. Methods Forty patients who underwent curative resection for NSCLC were enrolled. Before resection, 10-mL samples were obtained of PB from the radial artery, blood from the PV of the lobe containing the tumor, and BM aspirates from the rib. A microfabricated filter was used for CTC enrichment, and immunofluorescence staining was used to identify CTCs. Results The pathologic stage was stage I in 8 patients (20%), II in 15 (38%), III in 14 (35%), and IV in 3 (8%). The median number of PB-, PV-, and BM-CTCs was 4, 4, and 5, respectively. A time-dependent receiver operating characteristic curve analysis showed that PB-CTCs had excellent predictive value for recurrence-free survival (RFS), with the highest area under the curve at each time point (first, second, and third quartiles of RFS). In a multivariate Cox proportional hazard regression model, PB-CTCs were an independent risk factor for recurrence (hazard ratio, 10.580; 95% confidence interval, 1.637–68.388; p<0.013). Conclusion The presence of ≥4 PB-CTCs was an independent poor prognostic factor for RFS, and PV-CTCs and PB-CTCs had a positive linear correlation in patients with recurrence.

show abstract

“…Moreover, cancer cells highly expressing CD133 are invasive in vitro and associated with poor prognosis in patients 36 . Interestingly, the CD133 expression as a stem cell marker gradually decreases with cell differentiation 37 , which is likely linked to changes in cancer metabolism. CSCs have also been isolated from primary laryngeal cancer cell lines using CD133 as a screening marker 38 .…”

Section: Discussionmentioning

confidence: 99%

Characterisation of a subpopulation of CD133+ cancer stem cells from Chinese patients with oral squamous cell carcinoma

Zhang

Liu

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Cancer stem cells (CSCs) play a critical role in cancer development and growth. The aim of this study was to identify and isolate cScs from populations of primary oral squamous cell carcinoma (OSCC) cells, which were obtained from OSCC specimens and identified by cell morphology and immunohistochemical staining for keratin. CD133 + cells detected by flow cytometry comprised 0.41 ± 0.06% of primary OSCC cells and were isolated from primary OSCC cell populations using magnetic-activated cell sorting, revealing that 93.39% of high-purity CD133 + cells were in the G0/ G1 phase of the cell cycle. Additionally, the growth rate of CD133 + cells was higher than that of CD133 − cells, and in vivo tumourigenesis experiments showed that the tumourigenic ability of CD133 + cells was markedly stronger than that of CD133 − cells. Moreover, CD133 + cells showed increased chemotherapeutic resistance to cisplatin and higher self-renewal ability according to sphere-formation assay, as well as higher mRNA levels of stemness-associated genes, including NANOG, SOX2, ALDH1A1, and OCT4. These results indicated that OSCC cells, which share certain characteristics of CSCs, harbour CD133 + cells potentially responsible for OSCC aggressiveness, suggesting CD133 as a potential prognostic marker and therapeutic target. Head and neck cancer (HNC) is the sixth most common malignancy worldwide, with oral squamous cell carcinoma (OSCC) the most common type of HNC and accounting for >90% of all oral cancers 1,2. Smoking, drinking, and chewing areca nuts are considered risk factors for OSCC, which can affect any part of the mouth, including the lips, tongue, and throat 3. Furthermore, when patients develop OSCC, they experience maxillofacial deformity and psychological trauma in addition to common symptoms of cancer. Although there have been advances in surgical techniques and chemotherapeutic strategies for OSCC, the patient survival rate remains low 4. Cisplatin is the first-line chemotherapeutic drug currently used to treat patients with locally advanced, resectable OSCC; however, cisplatin resistance poses a major challenge for its clinical application 5 and is considered the critical cause of tumour recurrence in OSCC patients 6. Therefore, a better understanding of the mechanisms underlying OSCC recurrence is required to develop novel therapeutic strategies 7. Studies have increasingly focused on the roles of cancer stem cells (CSCs) in cancer invasion and recurrence 8. CSCs represent highly heterogeneous subpopulations with functional heterogeneity in their respective tumours and are characterised by infinite proliferation, self-renewal, chemotherapeutic resistance, and multidirectionality 9. The role of CSCs was previously demonstrated in the development and therapeutic resistance of liver cancer 10. Similar to their roles in other malignancies, CSCs also play a pivotal role in OSCC development and progression 11,12. The study of CSCs, also referred to as tumour-initiating cells 13 , has recently become among the most popular research dire...

show abstract

Clinicopathological and prognostic significance of circulating tumor cells in patients with lung cancer: a meta-analysis

Cited by 7 publications

References 39 publications

Diagnostic role of circulating extracellular matrix-related proteins in non-small cell lung cancer

Diagnostic role of circulating extracellular matrix-related proteins in non-small cell lung cancer

Prognostic Role of Circulating Tumor Cells in the Pulmonary Vein, Peripheral Blood, and Bone Marrow in Resectable Non-Small Cell Lung Cancer

Characterisation of a subpopulation of CD133+ cancer stem cells from Chinese patients with oral squamous cell carcinoma

Contact Info

Product

Resources

About