Clinicopathological role of miR-30a-5p in hepatocellular carcinoma tissues and prediction of its function with bioinformatics analysis

Abstract

BackgroundIt has been reported that deregulation or dysfunction of microRNAs (miRNAs) plays an essential part in the hepatocarcinogenesis. However, the contribution and mechanism of microRNA-30a-5p (miR-30a-5p) in hepatocellular carcinoma (HCC) remains largely unknown. Therefore, our aim was to investigate the clinicopathological role of miR-30a-5p in HCC tissues and explore its potential pathways in this study.MethodsThe expression of miR-30a-5p was measured in 95 HCC and adjacent noncancer tissues by real-time reverse transcription quantitative polymerase chain reaction. The relationship between miR-30a-5p expression levels and clinicopathological parameters was also analyzed. Furthermore, the potential target genes of miR-30a-5p were collected via online prediction and literature searching. Gene ontology and pathway enrichment analyses were used to identify the possible function of miR-30a-5p in HCC.ResultsCompared with adjacent noncancer tissues (2.23±0.77), expression level of miR-30a-5p was significantly lower in HCC tissues (1.26±0.66, P<0.001). MiR-30a-5p expression was evidently correlated with tumor nodes, metastasis, tumor–node–metastasis stage, portal vein tumor embolus, vascular invasion, and status of tumor capsule (all P<0.05). A total of 878 genes were finally used for the biological informatics analyses. These prospective target genes were highly enriched in various key pathways, for instance, Ubiquitin-mediated proteolysis, Axon guidance, Neurotrophin signaling pathway, Amyotrophic lateral sclerosis, and ErbB signaling pathway.ConclusionIn conclusion, this study clarifies that the downregulation of miRNA-30a-5p might play a vital part in the incidence and progression of HCC via targeting various prospective genes and pathways. Future validation is required to further explore the prospective molecular mechanism of miR-30a-5p in HCC.

RETRACTED: DDP-resistant ovarian cancer cells-derived exosomal microRNA-30a-5p reduces the resistance of ovarian cancer cells to DDP

“…From previous reports, miR-30a-5p is known to play an anti-oncogenic role in tumourigenesis. For instance, low expression of miR-30a-5p correlates with tumour metastasis, a high tumour stage, as well as tumour-capsule infiltration [20]. Additionally, induction of miR-30a-5p enhances drug sensitivity by inhibiting Beclin-1 in human small cell lung cancer [21].…”

Over-expression of Thrombospondin 4 correlates with loss of miR-142 and contributes to migration and vascular invasion of advanced hepatocellular carcinoma

“…Reduced expression of miR-142 was found in some types of cancers such as osteosarcoma and renal cancer [ 11 , 12 ], but this has not observed in HCC. Previous studies have demonstrated that miRNAs were involved in the regulation of HCC invasion and migration, for example, miR-30, miR-135, and miR-1299 [ 28 – 30 ]. In this study we identified that miR-124 suppresses HCC invasion and migration through down-regulation of THBS4 , and loss of miR-124 leads to over-expression of THBS4 thereby promoting HCC invasion and migration.…”

Evaluation and Application of Drug Resistance by Biomarkers in the Clinical Treatment of Liver Cancer

“…MiR-30a-5p plays vital roles in the carcinogenesis and progression of various malignancies via different molecular mechanisms [ 123 ]. miR-30a-5p expression is significantly lower in HCC tumor tissue compared with adjacent normal tissue and is correlated with tumor nodes, metastasis, the tumor–node–metastasis stage, portal vein tumor embolus, vascular invasion, and tumor capsule status [ 124 ]. The aberrant glucose metabolism in cancer cells, reflecting a shift away from oxidative phosphorylation towards aerobic glycolysis during tumorigenesis (i.e., Warburg effect), is associated with resistance to chemotherapeutic agents.…”