Clinicopathological Significance and Antitumor Effect of MPHOSPH1 in Testicular Germ Cell Tumor

Abe, Tetsutaro; Kohashi, Kenichi; Takemoto, Junkichi; Kinoshita, Fumio; Eto, Masatoshi; Oda, Yoshinao

doi:10.7150/jca.25279

Cited by 1 publication

(1 citation statement)

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“…The overall survival rates of SGCT and NSGCT patients are favorite. However, poor prognosis, high recurrence rates, and low survival can occur after surgery or chemotherapy (Abe et al, 2018; Liu et al, 2018). Therefore, it is necessary to explore novel biomarkers and therapeutic agents for TGCT treatment.…”

Section: Introductionmentioning

confidence: 99%

Retracted: Physcion 8‐O‐β‐glucopyranoside inhibits testicular germ cell tumors through regulating microRNA‐199a

Dou

Wang

Zhang³

et al. 2019

The Anatomical Record

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Testicular germ cell tumors (TGCTs) are frequently diagnosed solid tumors in young adult males. The present study aimed to investigate the anti-tumor function of Physcion 8-O-β-Glucopyranoside (PG) in TGCTs, and to explore the underlying anti-tumor mechanism of PG in TGCTs. Cell viability was measured by a cell counting kit-8 (CCK-8) assay, and the cell cycle distribution was analyzed by flow cytometry. Cell apoptosis was measured using TUNEL staining and flow cytometry. The lactate dehydrogenase (LDH) release, glucose consumption, lactate production, and ATP generation were determined using a Flex Bioanalyzer. The expression of miR-199a was measured by qRT-PCR, and the expression of marker proteins were analyzed by western blotting. Moreover, a xenograft mouse model was used for investigating PG's antitumor effect in vivo. The results showed that PG significantly inhibited NTERA2 and NCCIT cell proliferation, blocked the cell cycle, and induced cell apoptosis. Furthermore, PG suppressed LDH release, glucose consumption, lactate production, and ATP generation in NTERA2 and NCCIT cells. The results of investigating the molecular mechanism of PG in TGCTs showed that miR-199a was decreased in TGCTs but was increased by PG in a dose-dependent manner. MiR-199a knockdown significantly increased NTERA2 and NCCIT cell proliferation, decreased the apoptotic cell rates, and promoted LDH release, glucose consumption, lactate production, and ATP generation. The effects of PG on TGCTs were reversed by miR-199a silencing. In addition, we found that PG significantly inhibited tumor growth in vivo. In conclusion, our study revealed that PG modulated cell proliferation, the cell cycle, apoptosis, and glucose metabolism in TGCTs through regulating miR-199a. These findings indicate a novel therapeutic strategy and a potential biomarker for TGCT treatment.

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Section: Introductionmentioning

confidence: 99%