Cloacael Carriage and Multidrug Resistance Escherichia coli O157:H7 from Poultry Farms, Eastern Ethiopia

Abstract

A cross-sectional study was carried out to determine antimicrobial drug resistance patterns of E. coli O157:H7 isolates and estimate the level of the pathogen. A total of 194 cloacae swab samples were collected randomly in two poultry farms. Standard cultural, biochemical, and serological (latex agglutination) methods were used to isolate E. coli O157:H7. The isolates were subjected to antimicrobial susceptibility testing using disc diffusion method. Out of 194 cloacae samples examined, 13.4% (n = 26) were found to be positive for E. coli O157:H7. The finding indicated differences in E. coli O157:H7 infection among the different risk factors. Chicken from Adele Poultry Farm showed higher E. coli O157:H7 infection (OR = 3.89) than Haramaya University poultry farm and young birds had more infection (OR = 4.62) than adult birds. Of the total 14 antimicrobials included in the panel of study, the susceptibility results were varied with 96.15% and 0% E. coli O157:H7 isolates expressing resistance to erythromycin, clindamycin, spectinomycin, and ciprofloxacin, respectively. Multidrug resistance to more than two antimicrobial agents was detected in 24 (92.30%) of the isolates. The study showed high presence of antimicrobial resistant isolates of E. coli O157:H7. Further study is required to better understand the ecology and evolution of bacterial resistance to antimicrobial agents.

RETRACTED ARTICLE: Occurrence and antimicrobial susceptibility profile of Escherichia coli O157:H7 from food of animal origin in Bishoftu town, Central Ethiopia

“…Similarly, Hiko et al (2008) and Bekele (2012) from Ethiopia and Magwira et al (2005) from Botswana revealed that the resistance of E. coli does exist mainly to ampicillin and streptomycin. However, various authors reported that E. coli is resistant to tetracycline (Hiko et al 2008;Bekele 2012;Mude et al 2017), which is contrary to the results of the present study. But in Dire Dawa, Mohammed et al (2014) reported that E. coli was susceptible to tetracycline, which is in line with the present study finding.…”

“…This is in agreement with the report of Mude et al (2017), who showed 92.3% of isolates were multidrug resistant. Moreover, various authors (Bekele et al 2014;Iweriebor et al 2015;Atnafie et al 2017) from the country and abroad reported multidrug resistance patterns.…”

Isolation and antimicrobial susceptibility pattern of Escherichia Coli from laying chicken and egg in Jimma Town, Ethiopia

“…But this finding is lower than some reports from previous works (13). The prevalence of E. coli from cloacae in this study is in line with the privies studies [14,15]. In contrast the finding of this prevalence in the present study is higher than the study of [16,22,23,24 ] in which E. coli was isolated from cloacae samples taken from poultry farms.…”

“…In the previous study, which carried out the determination of isolation and antimicrobial drug resistance patterns of E. coli isolates and estimates, the level of the pathogen was connected at eastern Ethiopia on poultry farm of cloacae swabs sample and in different studies were conducted on the prevalence of E.coli from both markets and farm egg [8]. There is very little information regarding the E.coli profile from egg and cloacae of poultry and drug susceptibility patterns particularly in Jimma Town, south western Ethiopia.…”

<i>Campylobacter, Salmonella</i> and <i>Escherichia coli</i> Food Contamination Risk in Free-Range Poultry Production System

“…Other authors reported that E. coli was the most prevalent bacteria (53.50%) in poultry droppings in Akure, Ondo State, Nigeria [69]. Another research conducted in Ethiopia revealed that out of 194 cloacae samples examined for E. coli O157:H7, 13.4% were found to be positive for E. coli O157:H7 [39]. Free-range chicks aged 1 to 3 weeks have the highest prevalence (10.89%) of E. coli.…”