Clonal Analysis of Neisseria meningitidis Serogroup B Strains in South Africa, 2002 to 2006: Emergence of New Clone ST-4240/6688

Moodley, Chivonne; Plessis, Mignon du; Ndlangisa, Kedibone; Gouveia, Linda de; Klugman, Keith P.; Gottberg, Anne von

doi:10.1128/jcm.01079-12

Cited by 5 publications

(1 citation statement)

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“…MLVA is a PCR-based technique that utilizes the variability in the number of short tandem repeat sequences that is used to create DNA fingerprints and has been reported to have higher discriminatory power than MLST when employing suitable VNTR loci. MLVA has been successfully used as a sub typing method for several bacteria, such as Mycobacterium tuberculosis, Yersinia pestis, and Leptospira L-form [12] – [14] , and several studies investigating N. meningitidis with MLVA have been performed, particularly for serogroup C meningococci [15] – [19] . Therefore, we used MLVA in this study to analyze the microevolution of N. meningitidis serogroup C ST-4821 in the last decade.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Neisseria meningitidis Serogroup C ST-4821 in China Based on Multiple-Locus Variable Number Tandem Repeat Analysis

et al. 2014

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Neisseria meningitidis sequence type (ST)-4821 was first reported in China in 2003, and a new hyper-virulent lineage has been designated as the ST-4821 complex. A large number of N. meningitidis ST-4821 strains have been identified in China since 2003; however, the microevolution characteristics of this complex are unclear. Different combinations of variable number of tandem repeats (VNTR) loci were used in multiple-locus VNTR analysis (MLVA) to analyze 118 N. meningitidis serogroup C ST-4821 strains isolated from seventeen provinces between 2003 and 2012. Additionally, MLVA with five VNTR loci was performed due to its high discriminatory power. One hundred and eighteen isolates were found to comprise 112 subtypes based on MLVA, and 16 outbreak-associated strains were clustered into one group. These data indicate a high level of diversity for N. meningitidis ST-4821 due to microevolution in the last decade. In addition, the results revealed high similarity between isolates from the same geographic origins, which is helpful when monitoring the spread of N. meningitidis serogroup C ST-4821 and will provide valuable information for the control and prevention of bacterial meningitis in China.

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Section: Introductionmentioning

confidence: 99%