Clonal Dissemination of Clinical Isolates of Acinetobacter baumannii Carriers of 16S rRNA Methylase Genes in an Oncological Hospital in Recife, Brazil

Pereira, Jussyêgles Niedja da Paz; Andrade, Carlos Alberto das Neves; Lima, Jailton Lobo da Costa; Lima-Neto, Reginaldo Gonçalves de; Araújo, Paulo Sérgio Ramos de; Maciel, Maria Amélia Vieira

doi:10.1007/s00284-019-01786-6

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…Two randomized clinical trials have evaluated the mortality rate in patients infected by Gram-negative bacteria resistant to carbapenems and identified a 30% to 40% mortality associated with colistin in 28 days of treatment, whereas the mortality rates associated with the use of imipenem-relebactam and plazomicin were 10 and 12%, respectively [35,36]. erefore, the need for studies regarding alternative treatments is urgent [2,4,37]. e emergence of infections caused by resistant bacteria has encouraged the use of combined therapeutic approaches in the medical clinic.…”

Section: Synergistic Antimicrobial Activity Of Polymyxin B Andmentioning

confidence: 99%

“…The wide variety of virulence and resistance mechanisms presented by P. aeruginosa and Acinetobacter spp. isolates, as well as their capacity of interacting and exchanging genetic material with other bacteria, stimulates the search for resistance genes in isolates found in hospital environments [ 2 – 4 ]. Besides, the increasing number of infections caused by multidrug-resistant (MDR) Gram-negative strains led to the return of the polymyxins (polymyxin B and colistin) in medical clinics as one of the treatment options [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Synergistic Effect between Usnic Acid and Polymyxin B against Resistant Clinical Isolates of Pseudomonas aeruginosa

Júnior

Silva

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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The present study aimed to characterize the susceptibility profile of Pseudomonas aeruginosa and Acinetobacter spp. clinical isolates to polymyxin B in a public hospital in Recife-PE, Brazil, between the years of 2018 and 2019, as well as to search for the presence of the mcr-1 gene and evaluate the interaction between polymyxin B and usnic acid against these isolates. The strains were identified using the BD Phoenix™ automated system and the agar-spot test was used to determine the susceptibility profile to polymyxin B. The minimum inhibitory concentrations (MICs) of usnic acid and polymyxin B were determined through the broth microdilution method according to the Clinical and Laboratory Standards Institute (CLSI). Subsequently, Polymerase Chain Reaction (PCR) was performed to detect the mcr-1 gene in the isolates. The interaction between usnic acid and polymyxin B was evaluated by the Checkerboard assay. Among 34 isolates of P. aeruginosa, 26.5% (9/34) were positive for the polymyxin B agar-spot test, and 11.8% (4/34) presented an intermediate susceptibility (MIC = 4 μg/mL), while 14.7% (5/34) presented antimicrobial resistance with MIC values ranging from 8 to 32 μg/mL. Among 38 isolates of Acinetobacter spp., 13.2% (5/38) were positive for the polymyxin B agar-spot test and all of them were resistant to polymyxin B with a MIC value > 32 μg/mL. The mcr-1 gene was not detected in the clinical isolates. Regarding usnic acid, it presented a moderate antibacterial activity against two P. aeruginosa isolates (MIC = 250 μg/mL) and no activity was detected against the others. A synergistic effect between usnic acid and polymyxin B was observed against three clinical isolates of P. aeruginosa which were resistant to polymyxin B (FICI ≤ 0.5). Therefore, it was possible to observe that usnic acid is a promising candidate to be used in combination with polymyxin B against infections caused by resistant P. aeruginosa.

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Section: Synergistic Antimicrobial Activity Of Polymyxin B Andmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synergistic Effect between Usnic Acid and Polymyxin B against Resistant Clinical Isolates of Pseudomonas aeruginosa

Júnior

Silva

et al. 2020

Evidence-Based Complementary and Alternative Medicine

Self Cite

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“…The 16S-RMTase gene rmtC was firstly detected in P. mirabilis isolated from Japan in 2004 [ 63 ]. Since then, rmtC has been spread widely around the world and distributed in various species, such as K. pneumoniae , E. coli , P. aeruginosa, and A. baumanii [ 24 , 60 , 69 ], resulting in high-level resistance to aminoglycosides with MIC ≥ 1024 μg/mL [ 63 ]. According to the genetic sequences around the rmtC , there was an obvious pattern that IS Ecp1 was located upstream of rmtC and played an important role in the expression of rmtC as well as the transfer among different Gram-negative strains [ 60 , 63 ].…”

Section: Plasmid-mediated 16s Rrna Methylase Resistance Gene and Its ...mentioning

confidence: 99%

“…rmtB1E. coli 2019-Japan, China, Korea; 2020-India, China, French; 2021-China, Britain, Greece, Kenya; 2022-Switzerland[7,20,24,32, …”

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confidence: 99%

Research Updates of Plasmid-Mediated Aminoglycoside Resistance 16S rRNA Methyltransferase

Yang

2022

Antibiotics

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With the wide spread of multidrug-resistant bacteria, a variety of aminoglycosides have been used in clinical practice as one of the effective options for antimicrobial combinations. However, in recent years, the emergence of high-level resistance against pan-aminoglycosides has worsened the status of antimicrobial resistance, so the production of 16S rRNA methyltransferase (16S-RMTase) should not be ignored as one of the most important resistance mechanisms. What is more, on account of transferable plasmids, the horizontal transfer of resistance genes between pathogens becomes easier and more widespread, which brings challenges to the treatment of infectious diseases and infection control of drug-resistant bacteria. In this review, we will make a presentation on the prevalence and genetic environment of 16S-RMTase encoding genes that lead to high-level resistance to aminoglycosides.

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Emergence of rmtD1 gene in clinical isolates of Pseudomonas aeruginosa carrying blaKPC and/or blaVIM-2 genes in Brazil

et al. 2021

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Clonal Dissemination of Clinical Isolates of Acinetobacter baumannii Carriers of 16S rRNA Methylase Genes in an Oncological Hospital in Recife, Brazil

Cited by 5 publications

References 38 publications

Synergistic Effect between Usnic Acid and Polymyxin B against Resistant Clinical Isolates of Pseudomonas aeruginosa

Synergistic Effect between Usnic Acid and Polymyxin B against Resistant Clinical Isolates of Pseudomonas aeruginosa

Research Updates of Plasmid-Mediated Aminoglycoside Resistance 16S rRNA Methyltransferase

Emergence of rmtD1 gene in clinical isolates of Pseudomonas aeruginosa carrying blaKPC and/or blaVIM-2 genes in Brazil

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