Clonal Fidelity and Genetic Diversity of Micropropagated Hancornia speciosa Gomes (Apocynaceae) as Evaluated by Molecular Markers

Abstract

The plant tissue culture procedures for asexual multiplication of Hancornia speciosa represent an important process facilitating the preservation of selected genotypes of this threatened species and large-scale production of plantlets for population reinforcement or other utilization. However, there is no research regarding the somaclonal variation of this threatened species at molecular level. This study aimed to evaluate the role of the multiplication process in the genetic stability of H. speciosa plantlets and calluses using molecular markers. The tissue culture techniques for H. speciosa micropropagation used in this study did not influence the occurrence of somaclonal variation, which can be related to the genotypes of the donor plants and to the low concentrations of growth regulators used in tissue culture procedures adopted. The results observed here provide a reliable methodology for further studies involving micropropagation techniques with the goal of preserving selected or endangered genotypes of H. speciosa without genetic losses and producing seedlings of this species on a large scale. In addition, it was possible to verify that the donor plants used in this study showed a relatively low genetic variability.

Induction of Axillary Bud Swelling of Hevea brasiliensis to Regenerate Plants through Somatic Embryogenesis and Analysis of Genetic Stability

“…In this study, analysis using ISSR markers revealed a low level of polymorphism between the test samples. Similar results were observed in many previous reports [55][56][57]. In the genetic diversity assessments of Hevea ABRPs, BSs and SSESs using ISSR markers, 488 loci were amplified.…”

Advances and perspectives in the propagation of mangabeira (Hancornia speciosa Gomes) - review on a tropical fruit tree with socioeconomic importance

“…Other studies have shown that inoculation of bacterial isolates during mangabeira micropropagation influenced the number of buds and expanded leaves (Cabral et al, 2018). The occurrence of somaclonal variation during micropropagation of H. speciosa was also not observed (Costa et al, 2022).…”

“…• Micropropagation of H. speciosa did not influence the occurrence of somaclonal variation. (Pereira-Netto, 1996;Pereira-Netto, 2001;Machado et al, 2004;Soares et al, 2011;Sá et al, 2012;Oliveira et al 2016;Vieira et al, 2018;Cabral et al, 2018;Costa et al, 2022) Growth in vitro…”