Clonal Fidelity and Variation in Plantain (Musa AAB) Regenerated from Vegetative Stem and Floral Axis Tips in vitro

Krikorian, A. D.; Irizarry, Héber; Cronauer-Mitra, Sandra S.; Rivera, Edmundo

doi:10.1006/anbo.1993.1068

Cited by 43 publications

(25 citation statements)

References 20 publications

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“…Without specifying numbers, Krikorian et al (1993) suggested that excessive numbers of subcultures may increase the rate of somaclonal variation in vitro. Therefore, it is believed that the age of the culture is related to increased genetic instability.…”

Section: Introductionmentioning

confidence: 99%

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

Hsie¹,

Brito²,

Nova³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Sugarcane culture is an important source of income for the Brazilian economy. The aim of this study was to identify somaclonal variation in sugarcane varieties RB943365 and RB92579 arising from micropropagation using inter-simple sequence repeat (ISSR) DNA markers. The evaluated plants were generated from the in vitro propagation of shoot tips grown in MS medium supplemented with vitamins, myoinositol, glycine, and sucrose, without the use of growth regulators. Fifteen consecutive subcultures with intervals of 14 days were carried out, and DNA was extracted from young leaves obtained from each of the subcultures. The DNA was amplified with ISSR markers and separated by electrophoresis on 2% agarose gels. No evidence of polymorphism was observed in subcultures of the varieties analyzed, suggesting the absence of somaclonal variants. In this way, the ISSR marker was efficient at analyzing somaclonal variation, and in vitro propagation of sugarcane can be considered efficient for 15 consecutive subcultures of the varieties analyzed.

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Section: Introductionmentioning

confidence: 99%

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

Hsie¹,

Brito²,

Nova³

et al. 2015

Genet. Mol. Res.

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“…A mesma preocupação foi demonstrada por KRIKORIAN et al (1993), no controle do número de subcultivos, para a manutenção da qualidade da muda. Para REUVENI et al (1986) o número de plantas obtidas a partir de um ápice caulinar de uma triplóide AAA, não deve ultrapassar a 1.000 plantas, mantendo-se assim a qualidade genética da muda micropropagada.…”

Section: Resultsunclassified

“…Willians (grupo AAA). KRIKORIAN et al (1993) sem especificar números, sugeriram que possa ocorrer influência do número excessivo de subcultivos in vitro no aumento da taxa de variação somaclonal.…”

Section: Introductionunclassified

Variação somaclonal em mudas micropropagadas de bananeira, cultivar Pacovan

Santos

Rodrigues

2004

Bragantia

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RESUMOA literatura tem relatado a ocorrência de variação somaclonal em plantas derivadas de cultura de tecidos. Neste trabalho, foi estudada a influência do número de subcultivos na indução de variantes em bananeira, cultivar Pacovan (Musa spp., grupo AAB). Ápices caulinares foram introduzidos e multiplicados in vitro, utilizando-se no estabelecimento o meio de cultura MS com adição de 2,5 mg.L -1 de BAP e nos subcultivos subseqüentes, o meio MS com 4,0 mg.L -1 de BAP para indução de brotações laterais. Foram obtidas gerações com diferentes números de subcultivos (3, 4, 5, 6, 7, 8 e 9), e comparadas com mudas obtidas de rizomas. Observaram-se as plantas obtidas, no total de 426, em condições de campo no Vale do Açu (RN). Para cálculo da porcentagem de variantes somaclonais, avaliaram-se as seguintes caracterís-ticas morfológicas: estatura da planta, coloração e forma das folhas e conformação do cacho. Foram consideradas como variantes apenas as plantas cujos perfilhos também mostravam o mesmo tipo de variação. Constatou-se a ocorrência de variação somaclonal nas mudas provenientes dos tratamentos a partir de cinco subcultivos, atingindo-se o valor de 5,8% de variantes no nono subcultivo. O aumento da porcentagem de variação somaclonal com o do número de subcultivos indica o cuidado que as biofábricas devem ter em relação a esse parâmetro, desenvolvendo protocolos de micropropagação específicos para cada cultivar a ser comercializada.Palavras-chave: produção de mudas, Musa spp, in vitro, variação somaclonal. ABSTRACT SOMACLONAL VARIATION EVENT ON MICROPROPAGATED PACOVANBANANA SEEDLING (Musa spp. AAB group)The occurrence of somaclonal variation for plants in developed from tissue culture is well documented in the literature. The present study evaluated the influence of numbers subculture in the induction of variants in Pacovan banana tree (Musa spp., AAB group). Apex stems were introduced and multiplied in vitro using culture media MS, with addition of 2.5 mg.L -1 of BAP. In subsequent subcultures, MS with 4.0 mg.L -1 of BAP was used to induce side buds. Results showed that plants were regenerated with different numbers of subculture (3, 4, 5, 6, 7, 8, and 9). These data were compared with seedlings from developed rhizomes. All the plants, were observed at field conditions in Vale do Açu, Rio Grande do Norte State. The percentage of somaclonal variants, some was estimated using morphological characteristics, such as plant size, color and shape of leaves and bunch shape. Only plants in which buds

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“…Genetic fidelity largely depends on explant source [37]. The explant tissue can affect the frequency and nature of somaclonal variation [38,39].…”

Section: Explant Sourcementioning

confidence: 99%

Somaclonal Variation in Tissue Culture: A Case Study with Olive

Leva¹,

Petruccelli²,

Rinaldi³

2012

Recent Advances in Plant in Vitro Culture

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Clonal Fidelity and Variation in Plantain (Musa AAB) Regenerated from Vegetative Stem and Floral Axis Tips in vitro

Cited by 43 publications

References 20 publications

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

Determining the genetic stability of micropropagated sugarcane using inter-simple sequence repeat markers

Variação somaclonal em mudas micropropagadas de bananeira, cultivar Pacovan

Somaclonal Variation in Tissue Culture: A Case Study with Olive

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