Clonal growth and differentiation of mesenchymal stromal cells from rat liver at different stages of embryogenesis

Payushina, O. V.; Буторина, Н. Н.; Nikonova, T. M.; Kozhevnikova, M. N.; Sheveleva, O. N.; Старостин, В. И.

doi:10.1134/s1990519x12010075

Cited by 4 publications

(2 citation statements)

References 17 publications

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“…The amount of these cells in the liver changes during development in correlation with hematopoietic activity [65][66][67], which is evidence for their important role in organization of the hematopoietic microenvironment. The cessation of hematopoiesis in the liver is accompanied by a decrease in not only the number of MSCs but also in their proliferative activity [59] and differentiation potential [67].…”

Section: Mesenchymal Stromal Cells (Mscs)mentioning

confidence: 99%

Hematopoietic Microenvironment in the Fetal Liver: Roles of Different Cell Populations

Payushina

2012

ISRN Cell Biology

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Hematopoiesis is the main function of the liver during a considerable period of mammalian prenatal development. Hematopoietic cells of the fetal liver exist in a specific microenvironment that controls their proliferation and differentiation. This microenvironment is created by different cell populations, including epitheliocytes, macrophages, various stromal elements (hepatic stellate cells, fibroblasts, myofibroblasts, vascular smooth muscle and endothelial cells, mesenchymal stromal cells), and also cells undergoing epithelial-to-mesenchymal transition. This paper considers the involvement of these cell types in the regulation of fetal liver hematopoiesis.

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Section: Mesenchymal Stromal Cells (Mscs)mentioning

confidence: 99%

Hematopoietic Microenvironment in the Fetal Liver: Roles of Different Cell Populations

Payushina

2012

ISRN Cell Biology

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“…into similar while growth of hematopoietic stem cells of the cord blood or bone marrow, consequently this is related to all types of colony. 7 This fact evidences about the high proliferative potential of hematopoietic cells of fetal liver. Unique properties of hematopoietic precursor cells of fetal liver -much shorter than in comparison with other sources of cell cycle, 8 that has greatest significance from the viewpoint of effective repopulation of the hematopoietic organs while transplantation.…”

Section: Mgmjmsmentioning

confidence: 89%

Fetal Liver CD34+-Cells in Dynamic of Human Fetus Development

Priya¹,

Kukharchuk²

2014

MGM Journal of Medical Sciences

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Fetal liver stem cells are not homogeneous but represented mostly by polyploidy hepatocytes and hepatoblasts and likewise early hematopoietic cells. Both cells lately used in regenerative medicine. However, fact remains unclear that, in which period of gestation preferably produces liver parenchyma stem cells and hematopoietic stem cells. In this case practically there is absence of phenotypic characteristics of liver cells in the dyna mics of its development. Phenotype of cells reflects the status of its genome, therefore such information are necessary for further development of cell transplantation. Aim: To create CD34 + phenotypic map of fetal stem cells, isolated from abortive material (fetal liver) obtained by means of MTP in gestation periods from 6 to 20 weeks. Methods: Flow cytometry phenotypic characteristics of hematopoietic fetal stem cells: CD34 + cells. Results: Results show that maximum total amount of fetal liver cells could be isolated in the gestation period 20 weeks, minimum-in the gestation period of 6 to 7 weeks. Maximum absolute quantity of CD34 + cells observed in the period of gestation 20 weeks, minimum-in the period of gestation 6 to 7 weeks. Viability of the cells while using our method of isola tion is high and varies from 91.5 to 95.5%. The highest percent of CD34 + cells observed in the gestation period 11 weeks, and lowest-in the period of gestation 6 to 7 weeks. Conclusion: Obtaining results allows making preliminary conclusion, that optimal period for isolation of fetal liver hematopoietic CD34 + cells are 11 weeks and 18 to 20 weeks periods of gestation age.

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Hematopoietic microenvironment and the role of mesenchymal stromal cells in its organization

Payushina

2015

Biol Bull Rev

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Clonal growth and differentiation of mesenchymal stromal cells from rat liver at different stages of embryogenesis

Cited by 4 publications

References 17 publications

Hematopoietic Microenvironment in the Fetal Liver: Roles of Different Cell Populations

Hematopoietic Microenvironment in the Fetal Liver: Roles of Different Cell Populations

Fetal Liver CD34+-Cells in Dynamic of Human Fetus Development

Hematopoietic microenvironment and the role of mesenchymal stromal cells in its organization

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