Clonal growth of carp (Cyprinus carpio) T cells in vitro

Yamaguchi, Takuya; Katakura, Fumihiko; Shitanda, Satoshi; Niida, Yoshimitsu; Toda, Hideaki; Ohtani, Maki; Yabu, Takeshi; Suetake, Hiroaki; Moritomo, Tadaaki; Nakanishi, Teruyuki

doi:10.1016/j.dci.2010.09.007

Cited by 17 publications

(3 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cell markers expressed in monocytes, lymphocytes and T cells analysed in this study were previously reported [40], [45], [46], and each marker gene was expressed only in a specific cell type. Moreover, analysis of morphology and FCM of blood leukocytes have been reported in several fishes [47], [48], [49].…”

Section: Discussionmentioning

confidence: 75%

Cytokine Gene Expression in CD4 Positive Cells of the Japanese Pufferfish, Takifugu rubripes

Kono

Korenaga

2013

PLoS ONE

View full text Add to dashboard Cite

CD4+ T (Th) cells are a central component of the adaptive immune response and are divided into distinct sets based on their specific cytokine production pattern. Several reports have suggested that fish possess Th subset activity similar to that of mammals. The aim of the present study was to isolate CD4+ T cells from the blood of Japanese pufferfish, Fugu rubripes, and to characterize their cytokine expression profile. We produced a specific antibody against Fugu CD4 and performed cell sorting with the magnetic activated cell sorting system. Sorted Fugu CD4+ cells were characterized by morphology and expression analysis of cell marker genes. Fugu CD4+ cells expressed T-cell marker genes but not macrophage or B-cell marker genes. In addition, peripheral blood lymphocytes were stimulated with lipopolysaccharide (LPS), polycytidylic acid (polyI:C), concanavalin A (ConA) prior to sorting, and then Multiplex RT-PCR was used to examine the expression of Th cytokines by the stimulated Fugu CD4+ cells. LPS and polyI:C stimulation upregulated the expression of Th1, Th17 and Treg cytokines and downregulated the expression of Th2 cytokines. ConA stimulation upregulated the expression of all Th cytokines. These results suggest that fish exhibit the same upregulation of Th-specific cytokine expression as in mammals.

show abstract

Section: Discussionmentioning

confidence: 75%

Cytokine Gene Expression in CD4 Positive Cells of the Japanese Pufferfish, Takifugu rubripes

Kono

Korenaga

2013

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…After pelleting down of leukocytes by centrifugation, supernatant was discarded and 2 mL of distilled water was added to the cell pellet to lyse mature erythrocytes, then 2 mL of twice concentrated MEM was immediately added. Remaining blood leukocytes were washed twice by centrifugations with MEM (Yamaguchi et al, 2011).…”

Section: Preparation Of Hematopoietic Cells From Carp Kidney Spleen mentioning

confidence: 99%

“…Blood leukocytes were prepared as described previously (Yamaguchi et al, 2011). In short, blood was withdrawn from the caudal vein from fish with heparinized syringe and transferred into a capillary glass tubes.…”

Section: Preparation Of Hematopoietic Cells From Carp Kidney Spleen mentioning

confidence: 99%

Exploring erythropoiesis of common carp (Cyprinus carpio) using an in vitro colony assay in the presence of recombinant carp kit ligand A and erythropoietin

Katakura

Yabu

Yamaguchi

et al. 2015

Developmental & Comparative Immunology

Self Cite

View full text Add to dashboard Cite

Effects of single or conjoint administration of lactic acid bacteria as potential probiotics on the growth, immune responses, and disease resistance of Carassius auratus

Chi

et al. 2022

Aquacult Int

View full text Add to dashboard Cite

Clonal growth of carp (Cyprinus carpio) T cells in vitro

Cited by 17 publications

References 37 publications

Cytokine Gene Expression in CD4 Positive Cells of the Japanese Pufferfish, Takifugu rubripes

Cytokine Gene Expression in CD4 Positive Cells of the Japanese Pufferfish, Takifugu rubripes

Exploring erythropoiesis of common carp (Cyprinus carpio) using an in vitro colony assay in the presence of recombinant carp kit ligand A and erythropoietin

Effects of single or conjoint administration of lactic acid bacteria as potential probiotics on the growth, immune responses, and disease resistance of Carassius auratus

Contact Info

Product

Resources

About