Clonal Multiplication ofNicotiana rustica L. from Shoot Meristems in Culture

Walkey, D. G. A.; Woolfitt, J. M. G.

doi:10.1038/2201346a0

Cited by 18 publications

(4 citation statements)

References 7 publications

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“…Wimber (1963) found that the process occurred more efficiently if the apex was grown in agitated liquid media where the proliferating mass would bceak up into individual~rotocorms, which would then repeat the process. This same technique has now been successfully applied to a number of species including carnation (Hackett and Anderson 1967) and Nicotiana rustica (Walkey and Woolfitt 1968). Although this method seems not to have been successf~~l thus far with trees, it would obviously find its greatest application in the propagation of comn~ercially valuable slow-to-mature woody species.…”

Section: Vegetative Propagationmentioning

confidence: 89%

The use of cultured tissue in studying developmental problems

Halperin¹

1973

Can. J. Bot.

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Section: Vegetative Propagationmentioning

confidence: 89%

The use of cultured tissue in studying developmental problems

Halperin¹

1973

Can. J. Bot.

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“…Each of the viruses was maintained in proliferating units of N . rustica in shake cultures at 22 "C ; the stock cultures were established as described by Walkey & Woolfitt (1968), except that the tips were taken from virus-infected N. rustica plants. Each stock culture was subcultured every 14 days into 25 ml of fresh culture medium contained in a 100 ml conical flask (Walkey & Cooper, 1972).…”

Section: Materials a N D M E T H O D Smentioning

confidence: 99%

High temperature inactivation of cucumber and alfalfa mosaic viruses in Nicotiana rustica cultures

Walkey¹

1976

Ann Applied Biology

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SUMMARY Cucumber mosaic (CMV) and alfalfa mosaic (AlfMV) viruses could not be detected in Nicotiana rustica tissues cultured at 32 °C for 16–18 days or at 40 °C for 5 days, but infectivity remained high in comparable tissue cultured at 22 °C. Incubation of infected cultures at 28–30 °C resulted in an initial reduction followed by a partial recovery in the infectivity of both viruses. The infectivity of CMV in tissues grown between 12 and 32 °C was highest in cultures grown at 12 °C. Although CMV infectivity was not detected in cultures after 16–18 days at 32 °C, virus was eliminated only after a further 30 days at 32 °C. When cultures were transferred from 32 to 22 °C after shorter treatment periods, infectivity rapidly increased to levels higher than those of infected tissues grown continuously at 22 °C. At 40 °C, CMV was eliminated from infected tissues after 9 days and AlfMV after 7 days. Cultures grown continuously at 40 °C deteriorated rapidly but, when grown under diurnal alternating periods of 8 h at 40 °C and 16 h at 22 °C, they remained viable and CMV was also inactivated.

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“…Infected tips were cultured in constant temperature cabinets either in static tubes on filter-paper bridges or in moving flasks as previously described (Walkey & Woolfitt, 1968;Walkey & Cooper, 1972).…”

Section: Culture Of Source Plants and Tipsmentioning

confidence: 99%

Effect of temperature on virus eradication and growth of infected tissue cultures

Walkey¹,

Cooper²

1975

Annals of Applied Biology

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Cucumber mosaic (CMV) and alfalfa mosaic (AlfMV) viruses were eradicated or their concentrations greatly diminished when infected meristem tips were grown in static or shake culture at 32 or 34 "C. Similar cultures grown at 22 "C remained infected. Tobacco mosaic virus (TMV) was not eradicated from shake cultures at 32 "C and the concentration of TMV in such cultures at 32 "C was sometimes higher than at 22 "C.The concentration of CMV and AlfMV was considerably less in cultured tissues than in infected leaf material, but that of TMV was similar. Cultures infected with CMV or TMV grew more slowly than healthy cultures. Growth of AlfMV infected and healthy cultures was similar.

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Clonal Multiplication ofNicotiana rustica L. from Shoot Meristems in Culture

Cited by 18 publications

References 7 publications

The use of cultured tissue in studying developmental problems

The use of cultured tissue in studying developmental problems

High temperature inactivation of cucumber and alfalfa mosaic viruses in Nicotiana rustica cultures

Effect of temperature on virus eradication and growth of infected tissue cultures

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