Clonal origin of haematopoietic colonies in the postnatal mouse liver

Rossant, Janet; Vijh, K. M.; Grossi, CE; Cooper, Max D.

doi:10.1038/319507a0

Cited by 40 publications

(34 citation statements)

References 7 publications

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“…The fetal hemopoietic liver as a source of myeloid precursor cells is well established [42], and fetal liver cells have successfully been used to restore granulopoiesis and monocytopoiesis in transplanted animals [43, 441. In addition, hemopoietic activity of the liver even up to 14 days of postnatal development has recently been reported to result in clonal hemopoietic foci and mixed colonies of erythroid cells and "Ma-like" accessory cells [45]. Infection of mice with oncogene-carrying retroviruses led to the establishment of leukemic, monocyte-like tumors in blood and liver.…”

Section: Discussionmentioning

confidence: 98%

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Liver‐associated macrophage precursor cells proliferate under impairment of regular hemopoiesis

Decker¹,

Lohmann‐Matthes²,

Baccarini³

1988

Eur J Immunol

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We reported previously that immature macrophage precursor cells can be isolated from spleen and liver of cyclophosphamide or pyran copolymer-pretreated mice. We now extended our investigations to livers of normal, untreated specific pathogen-free mice. Using the response to the macrophage growth factor colony-stimulating factor-1 (CSF-1) and the presence of the mouse macrophage-specific F4/80 antigen as criteria of definition, in the liver of normal mice we could demonstrate macrophage precursor (M phi P) cells by means of proliferation assays and flow cytometric analysis. The amount of M phi P present in the normal liver was significantly increased after administration of pyran copolymer. Also an enhanced proliferative response to CSF-1 as well as augmented natural killer activity and cytostasis of Candida albicans was noted in liver nonparenychymal cells (LNPC) after treatment of bone marrow (BM)-irradiated, splenectomized mice with pyran copolymer. Since the irradiated BM was actually proven to be silent by assessment of BM number and proliferative capacity and by scoring white blood cells, our findings suggest a response of endogenous liver M phi P under the applied conditions. Further evidence for the presence of endogenous liver hemopoietic cells was obtained from transplantation experiments in which LNPC brought about the survival of lethally irradiated mice. The data point towards a significance of the liver in disposing hemopoietic cells to the organism under impairment of regular hemopoiesis.

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Section: Discussionmentioning

confidence: 98%

“…Cells were incubated either in medium alone or with F4/80 mAb for 45 (15 x lo6 in 0.5 ml RPMI 1640) 2 h after irradiation. When indicated the mice received 500 pg pyran copolymer together with the cell suspension.…”

Section: Staining and Flow Cytometry Analysismentioning

confidence: 99%

Liver‐associated macrophage precursor cells proliferate under impairment of regular hemopoiesis

Decker¹,

Lohmann‐Matthes²,

Baccarini³

1988

Eur J Immunol

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“…In this way, 'oligoclones' would develop around individual macrophages before their exit into the circulation. Experimental evidence for this possibility has been obtained in studies of postnatal liver, using mouse chimeras (Rossant et al 1984), and has been inferred by the observation that erythroblasts often appear to be synchronized in erythroblastic islets isolated from regenerating adult haematopoietic tissues and foetal liver (Bessis et al 1978;Morris et al 1988). Similarly, with the mouse thymus, Kyewski & coworkers (1982) demonstrated that 'rosettes' of thymocytes attached to a central macrophage could be isolated by enzymic dispersion.…”

Section: Characterization Of Haematopoietic Cell Clustersmentioning

confidence: 96%

Novel cell surface adhesion receptors involved in interactions between stromal macrophages and haematopoietic cells

Crocker

Morris

Gordon

1988

Journal of Cell Science

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SummaryImmunocytochemical staining of tissues with the mouse macrophage-specific monoclonal antibody, F4/80, has shown that large numbers of stromal macrophages are present in adult and foetal haematopoietic tissues. Macrophage plasma membrane processes are seen to establish extensive associations with myeloid and erythroid cells in adult bone marrow and with developing erythroblasts in foetal liver, suggestive of local trophic interactions. To explore the nature of these interactions, methods were developed for isolation of resident bone marrow macrophages (RBMM) and foetal liver macrophages (FLM). Following collagenase digestion of bone marrow or foetal liver, clusters were obtained which were composed of one or more central macrophages surrounded by proliferating haematopoietic cells. After attachment of clusters to glass coverslips, adherent macrophages could be stripped free of haematopoietic cells by pipetting in the absence of divalent cations. The purified RBMM, but not FLM, expressed a novel haemagglutinin, which mediated binding, without ingestion, of large numbers of unopsonized sheep erythrocytes by a divalent cation-independent mechanism. In view of the possibility that this sheep erythrocyte receptor (SER) could interact with a homologous ligand on mouse bone marrow cells, its properties were examined. SER was found to be a lectin-like protein which recognized protease-resistant sialylated glycoconjugates on sheep erythrocytes. The expression of SER was restricted to certain stromal tissue macrophages and was low or absent on monocytes and macrophages obtained from serous cavities. High levels of SER could be induced on elicited peritoneal macrophages by cultivation in mouse serum and the induced receptor was found to mediate low-avidity binding of murine bone marrow cells with characteristics indistinguishable from those seen for binding of sheep erythrocytes. However, maximal binding of bone marrow cells to RBMM depended on a distinct, divalent cation-dependent adhesion system. Using erythroblasts as a ligand, FLM were selected to explore the properties and expression of this adhesion receptor, the erythroblast receptor (EbR). Similar to SER, EbR did not mediate ingestion, and was restricted in its expression to foetal and adult stromal tissue macrophages. Unlike SER, EbR activity was not affected by neuraminidase treatment of the ligand and the receptor was not induced on peritoneal macrophages cultured in mouse serum. EbR appears to be a novel cell adhesion receptor because it was unaffected by inhibitors of several previously described cell adhesion molecules, including the fibronectin receptor. Future studies will attempt to explore the functional significance of these two receptors in macrophage-haematopoietic cell interactions.

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“…Alternate sections were hybridized with biotin-labelled nick-translated plasmids containing 1400 bp of the M. musculus major satellite DNA sequence (C. Davis, unpublished data) or a 900 bp fragment of the M. caroli major satellite sequence (G. Fraser & J. Rossant, unpublished data). Conditions of hybridization were essentially as previously described (Rossant et al 1986). Hybridization was visualized by binding of streptavidin-biotin-horseradish peroxidase complex (Enzo Biochem) to the biotinylated DNA.…”

Section: Methodsmentioning

confidence: 99%

Cell-autonomous action of the testis-determining gene: Sertoli cells are exclusively XY in XX ↔XY chimaeric mouse testes

Burgoyne¹,

Buehr²,

Koopman³

et al. 1988

Development

Self Cite

178

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The distribution of XX and XY cells in XX—XY chimaeric mouse testes was analysed by enzyme marker analysis of separated testicular tissues and by in situ DNA marker analysis of air-dried testicular cells and testis sections. XX cells contributed to the Leydig cells, the peritubular cells and the vascularized connective tissue of the tunica albuginea. The Sertoli cells, on the other hand, appeared to be exclusively XY. These results indicate that during the development of the testis, Sertoli cell differentiation is triggered by cell-autonomous activity of the Y chromosomal testis-determining gene Tdy. Subsequent steps in testis differentiation may be a consequence of Sertoli cell activity.

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Clonal origin of haematopoietic colonies in the postnatal mouse liver

Cited by 40 publications

References 7 publications

Liver‐associated macrophage precursor cells proliferate under impairment of regular hemopoiesis

Liver‐associated macrophage precursor cells proliferate under impairment of regular hemopoiesis

Novel cell surface adhesion receptors involved in interactions between stromal macrophages and haematopoietic cells

Cell-autonomous action of the testis-determining gene: Sertoli cells are exclusively XY in XX ↔XY chimaeric mouse testes

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