Clonal propagation of Crataegus monogyna Jacq. (Lindm.)

Wawrosch, Christoph; Prinz, Sonja; Soleiman, Y.; Kopp, B.

doi:10.1055/s-2007-987397

Cited by 2 publications

(2 citation statements)

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“…(Lindm.) reported by Wawrosch et al 2007. Nikam et al (2008 (2011) reported an efficient protocol for multiple shoot induction in Ceropegia juncea.…”

Section: Propagation By Shoot and Node Culturementioning

confidence: 99%

In Vitro Micropropagation of Medicinal and Aromatic Plants

Máthé

Hassan

Kader

2015

Medicinal and Aromatic Plants of the World

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Modern biotechnological methods like in vitro micropropagation technique hold tremendous potential for the production of high-quality plant-based medicine. They also allow to achieve the large scale multiplication of diseasefree plants, faster cloning and the conservation of desired genotypes, in a very short span of time. Via genetic transformation techniques, the modification of both genetic information of MAPs and the regulation of genes responsible for the production of valuable biologically active substances has also become possible in either higher amounts or with better properties.Micropropagation protocols are worked out for many plant species cultured in vitro to provide macro-and micro-mineral nutrients, vitamins, source of carbohydrates, appropriate environmental conditions (light intensity, photoperiod and temperature) and plant growth regulators required to obtain high regeneration rates. As such they are expected to facilitate commercially feasible micropropagation. Well-defined cell culture methods have also been developed to produce pharmacologically important secondary metabolites. Genetic engineering is applied to produce transgenic medicinal plants and metabolites.This chapter offers a brief insight into the present status of the biotechnology of MAPs. Special emphasis has been placed on the in vitro micropropagation and rapid clonal multiplication of selected elite genotypes, the regulation of organogenesis and somatic embryogenesis, the exploitation of advantages in somaclonal variation and genetic engineering techniques for both crop improvement and in vitro germplasm conservation. The production of flavor and volatile constituents in tissue cultures will also be briefly discussed, similarly to the application of farther biotechnological approaches.

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“…(Lindm.) reported by Wawrosch et al 2007. Nikam et al (2008 (2011) reported an efficient protocol for multiple shoot induction in Ceropegia juncea.…”

Section: Propagation By Shoot and Node Culturementioning

confidence: 99%

In Vitro Micropropagation of Medicinal and Aromatic Plants

Máthé

Hassan

Kader

2015

Medicinal and Aromatic Plants of the World

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“…on the stage of multiplication and receipt of regenerant plants in vivo acclimatized. According to the scientific writings, various scientists have researched the matters of the various species of hawthorn microclonal propagation (Iapichino and Airo, 2009;Wowtosh et al, 2007;Dai et al, 2007). These authors use the apical and auxiliary meristema (Caboni et al, 2010) or single-bud segments of the lignified shoot (Gökbunar, 2007) as the explants.…”

Section: Introductionmentioning

confidence: 99%

Microclonal Propagation of Crataegus Monogyna Jacq. In Vitro

Zarnadze

Dolidze

Manjgaladze

et al. 2019

CBUP

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We have researched Crataegus Monogina Jacq. microclonal propagation and in vitro mass regeneration processes. For this experiment we used the asleep buds isolated from maternal plants and in vitro cultivated apical and axillary buds as the effective explants. The high coefficient of micropropagation was provided with Gamborg Medium (B5) nutrient medium added alongside the hormones of cytokinin nature. The best result was achieved in the case of introduction of 2-Isopentenyladenine (2-iP) at concentration of 5-10 mcm and Benzilaminopurine (BAP) at concentration of 10-15 mcm. For stimulation of the mictropropagation process it is reasonable to add a small dose of the auxin hormone 1-Naphtylacetic acid (NAA) together with the cytokinins to the nutritive medium. It has been shown that the growth of micropropagation coefficient in pro rata the kinds and concentration of phytohormones. Regenerated microshoots had a good capacity of rooting. The most effective method for rooting purpose was the introduction of Indole-3 -butyric acid (IBA) at concentration of 5mcm into 1 /2 B5 nutrient medium. The rooted regenerant plants successfully adopted in vivo.

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Clonal propagation of Crataegus monogyna Jacq. (Lindm.)

Cited by 2 publications

References 0 publications

In Vitro Micropropagation of Medicinal and Aromatic Plants

In Vitro Micropropagation of Medicinal and Aromatic Plants

Microclonal Propagation of Crataegus Monogyna Jacq. In Vitro

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