Clonal propagation of Rosa clinophylla Thory. through axillary bud culture

Misra, Pratibha; Chakrabarty, Debasis

doi:10.1016/j.scienta.2008.07.028

Cited by 18 publications

(15 citation statements)

References 17 publications

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“…In this treatment, on an average, 19.6 shoots having an average of 5.3-cm height and 8.5 leaves per shoot were formed ( Table 1). The stimulatory effect of GA on internodal elongation is well documented in literature [10][11][12][13]. It is pertinent to mention that in our study, the rate of regeneration of shoots is quite higher, i.e., 19.6 shoots per explant as compared with only 7.16 shoots per nodal explant as reported earlier [6].…”

Section: Resultssupporting

confidence: 76%

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Rapid In Vitro Production of Cloned Plants of Uraria picta (Jacq.) DC—A Rare Medicinal Herb in Long-Term Culture

Kumar

Sharma

Jain

et al. 2010

Appl Biochem Biotechnol

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An efficient in vitro process for rapid production of cloned plants of Uraria picta has been developed employing nodal stem segments taken from field-grown plants. Explants showed bud-break followed by regeneration of shoots with restricted growth within 12 days on modified Murashige and Skoog's medium supplemented with 0.25 mg l(-1) each of 6-benzylaminopurine and indole-3-acetic acid and 25 mg l(-1) adenine sulfate. Normal growth of shoots with good proliferation rate was achieved by reducing the concentrations of 6-benzylaminopurine and indole-3-acetic acid to 0.1 mg l(-1) each and incorporating 0.5 mg l(-1) gibberellic acid in the medium in which, on an average, 19.6 shoots per explant were produced. Further, during successive subcultures, increased concentrations of adenine sulfate (50 mg l(-l)) and gibberellic acid (2 mg l(-l)) along with the addition of 20 mg l(-l) DL: -tryptophan were found conducive to control the problem of necrosis of shoots. In this treatment, several "crops" of shoots were obtained from single culture by repeated subculturing of basal portion of stalk in long-term. Isolated shoots rooted 100% in 0.25 mg l(-1) indole-3-butyric acid. In vitro-raised plants after hardening in inorganic salt solution grew normally in soil and came to flowering. Genetic fidelity of in vitro-raised plants was ascertained by rapid amplified polymorphic DNA (RAPD) markers. Also, quantitative estimation of two isoflavonones in their root extracts further confirmed true-to-type nature of plantlets.

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Section: Resultssupporting

confidence: 76%

“…The data are presented in Table 4. The purity (99%) of estimated compounds was further confirmed by comparison with published data of standard (1) and (2) by UV, 1 HNMR, 13 CNMR, and ESI-MS [5,28] and was found identical. This study further ensures the genetic fidelity of plantlets regenerated through in vitro culture.…”

Section: Segment Nomentioning

confidence: 55%

Rapid In Vitro Production of Cloned Plants of Uraria picta (Jacq.) DC—A Rare Medicinal Herb in Long-Term Culture

Kumar

Sharma

Jain

et al. 2010

Appl Biochem Biotechnol

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“…The best treatment for shoot tip is 5%-40 min [0.2625% (w/v) NaOCl], a treatment comparable to Jo et al [30] who used about 0.15% (w/v) of NaOCl with 30 min exposure for shoot tip of Alocasia amazonica. In contrast, Misra and Chakrabarty [22] used higher concentration, 4% (w/v) NaOCl, and shorter exposure time, 5 min, to sterilize Rosa clinophylla shoot tip.…”

Section: Explant Surface Sterilizationmentioning

confidence: 99%

“…One drop of Tween-20 was added to the Clorox solution prior to sterilization. After each treatment, the explants were thoroughly washed with sterile deionized water 3 times [22], each comprising 10 min. After the sterilization steps above and prior to culturing on the nutrient medium, each node, internode and shoot tip explants were trimmed into 1 cm length.…”

Section: Explant Surface Sterilizationmentioning

confidence: 99%

Sterilization of <i>Hibiscus rosa-sinensis</i> L. Vegetative Explants Sourced from Plants Grown in Open Environment and Influences of Organic Ingredients on <i>In Vitro</i> Direct Regeneration

Tiong¹,

Ong

Namasivayam³

et al. 2012

AJPS

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This paper reports on the effects of organic ingredients in facilitating direct shoot regeneration from nodal explants of Hibiscus rosa-sinensis L. This paper also compares the sterilization conditions for 3 types of explants (node, internode, and shoot tip) harvested from an open field. The optimized sterilization conditions for the explants were 40% Clorox-20 min exposure, 10% Clorox-15 min exposure, and 5% Clorox-40 min exposure for the node, internode and shoot tip, respectively. In the direct shoot regeneration using the nodal explants, we found MS medium containing 40 g/L sucrose, 0.3% (w/v) activated charcoal, and supplementations with myo-inositol, thiamine and nicotinic acid were suitable. The in vitro shoot survival rate was 30% with a mean leaf numbers of 2.68 produced, and a mean leaf length of 1.71 cm achieved after 5 weeks of culture on the modified medium.

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“…GA 3 is also known to promote ethyleneinduced necrosis and leaf abscission (Morgan, 1976), which can be a particular problem for cultures in sealed vessels. However, use of ethylene inhibitors such as cobalt(II) chloride hexahydrate (CoCl 2 ), or silver nitrate (AgNO 3 ) can be effective for mediating the negative impacts of GA 3 -promoted ethylene (Ma et al, 1998;Misra and Chakrabarty, 2009).…”

mentioning

confidence: 99%

Micropropagation of Mahonia ‘Soft Caress’

Rounsaville¹,

Touchell²,

Ranney³

et al. 2011

horts

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Mahonia ‘Soft Caress’ is a unique new cultivar exhibiting a compact form and delicate evergreen leaves. Protocols for micropropagation of M. ‘Soft Caress’ were developed to expedite multiplication and serve as a foundation for future work with other taxa of Mahonia Nutt. Combinations of sucrose at 30 or 45 g·L−1 in conjunction with Gamborg B5 (B5), Quoirin and Lepoivre (QL), and Murashige and Skoog (MS) basal media as well as other selected growth regulator treatments were evaluated as multiplication media. Rooting of microcuttings was conducted in vitro using combinations of indole-3-butyric acid (IBA) at 0, 2, 4, 8, or 16 μM under either light or dark. Quick dip treatments with aqueous solutions of the potassium (K) salt (K-salt) of IBA at 0, 5.2, 10.4, 20.7, or 41.4 μM were tested in a second experiment for ex vitro rooting. Media containing B5 basal salts and vitamins supplemented with sucrose at 30 g·L−1, 5 μM 6-benzylaminopurine, 5 μM kinetin, 0.5 μM indole-3-acetic acid, and 2.5 μM gibberellic acid yielded 2.80 ± 0.14 microshoots with a mean length of 14.76 ± 0.63 mm over a 6-week culture period and was an optimal multiplication media. Light treatment and IBA concentration had a significant effect on rooting percentages. Microcuttings treated with 8 μM IBA and maintained in the dark resulted in the best rooting (70%) and ex vitro establishment.

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Clonal propagation of Rosa clinophylla Thory. through axillary bud culture

Cited by 18 publications

References 17 publications

Rapid In Vitro Production of Cloned Plants of Uraria picta (Jacq.) DC—A Rare Medicinal Herb in Long-Term Culture

Rapid In Vitro Production of Cloned Plants of Uraria picta (Jacq.) DC—A Rare Medicinal Herb in Long-Term Culture

Sterilization of <i>Hibiscus rosa-sinensis</i> L. Vegetative Explants Sourced from Plants Grown in Open Environment and Influences of Organic Ingredients on <i>In Vitro</i> Direct Regeneration

Micropropagation of Mahonia ‘Soft Caress’

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Clonal propagation of Rosa clinophylla Thory. through axillary bud culture

Cited by 18 publications

References 17 publications

Rapid In Vitro Production of Cloned Plants of Uraria picta (Jacq.) DC—A Rare Medicinal Herb in Long-Term Culture

Rapid In Vitro Production of Cloned Plants of Uraria picta (Jacq.) DC—A Rare Medicinal Herb in Long-Term Culture

Sterilization of &lt;i&gt;Hibiscus rosa-sinensis&lt;/i&gt; L. Vegetative Explants Sourced from Plants Grown in Open Environment and Influences of Organic Ingredients on &lt;i&gt;In Vitro&lt;/i&gt; Direct Regeneration

Micropropagation of Mahonia ‘Soft Caress’

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Sterilization of <i>Hibiscus rosa-sinensis</i> L. Vegetative Explants Sourced from Plants Grown in Open Environment and Influences of Organic Ingredients on <i>In Vitro</i> Direct Regeneration