Clonal relatedness and biofilm formation of OXA-23-producing carbapenem resistant Acinetobacter baumannii isolates from hospital environment

Aliramezani, Amir; Douraghi, Masoumeh; Hajihasani, Azade; Mohammadzadeh, Mona; Rahbar, Mohammad

doi:10.1016/j.micpath.2016.08.034

Cited by 20 publications

(16 citation statements)

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“…A. baumannii might first spread from infected patients or colonized personnel/visitors and then contaminate surfaces, thereby acting as prolonged sources for HAIs. Several studies suggested that A. baumannii could colonize different medical equipment and surfaces (11,21,22) and also could persist for years in patients (23) with the ability of biofilm formation (11). In the present study, we observed a significant MDR (21.9%) and XDR (18.8%) frequency.…”

Section: Discussionsupporting

confidence: 59%

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Genetic Diversity of OXA Producing Carbapenem-Resistant Acinetobacter baumannii from Environment of Tertiary Hospitals in Central Iran

Rostami

Mehrban

Ghourchian

et al. 2020

Arch Clin Infect Dis

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Background:The hospital environment usually involves in cross-colonization and/or outbreaks of carbapenem-resistant Acinetobacter baumannii (CRAB). This study aims to identify genetic diversity of environmental Acinetobacter baumannii (A. baumannii) isolates based on OXA-type carbapenems. Objectives: It was intended to identify the environmental source of A. baumannii strains to control future infections and ongoing outbreaks. Methods: Swab samples were collected from equipment, fluids and surfaces of intensive care units of five hospitals (H1 to H5). The susceptibility of the isolates was defined through disk diffusion method. blaOXA-51, blaOXA-58, blaOXA-23, and blaOXA-24 genes were detected by multiplex polymerase chain reaction (PCR) . Genetic diversity was investigated using ERIC-PCR. Mean values were compared using an Independent samples t-test. Results:The mean number of Gram-negative bacilli colony per sample was significantly higher in moist surfaces (1.9 ± 2.37) than in dry surfaces and medical equipment (P < 0.05). Among 32 A. baumannii isolates, 17 (53.1%) were classified as CRAB, 13 (40.6%) as multidrug resistant (MDR) and six (18.8%) as extremely drug resistant (XDR) phenotypes. The isolates showed the most susceptibility to tigecycline (81.3%) and doxycycline (81.3%). Totally, 10 isolates (31.3%) carried blaOXA-23 and one isolate carried blaOXA-58, but none contained blaOXA-24. Typing of the strains provided seven single types (ST) and nine clusters (A-I). Two isolates in cluster B (different surfaces of H4) and two in cluster I (from ventilators of H3 and H1) were designated as common type (CT). In H4 and H1, different isolates belonging to different clusters were recovered from bed sheet and baby sink bath, respectively. Conclusions: Clonally-related blaOXA-23 positive CRABs occurred in the environment, especially on moist surfaces of hospitals. The distribution of the same clones in different hospitals may point to expansion of a specific clone which increases the risk of crosscolonization of vulnerable patients.

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Section: Discussionsupporting

confidence: 59%

“…The standard phenol-chloroform extraction procedure was followed for genomic DNA extraction (11). A multiplex PCR was used to detect bla OXA-58 , bla OXA-24 , and bla OXA-23 genes in A. baumannii isolates, as described previously (12).…”

Section: Multiplex Pcr For Detection Of Oxa-type Carbapenemases Genesmentioning

confidence: 99%

Genetic Diversity of OXA Producing Carbapenem-Resistant Acinetobacter baumannii from Environment of Tertiary Hospitals in Central Iran

Rostami

Mehrban

Ghourchian

et al. 2020

Arch Clin Infect Dis

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“…The reported OXA-23 gene carrying isolates rate of A. baumannii were 52% and 85.6% in 2016, according to studies carried out in a referral hospital in the South and SouthWest of Iran, respectively (6,20). OXA-23 producing isolates is considered a significant cause of A. baumannii outbreaks worldwide (24,25).…”

Section: Discussionmentioning

confidence: 99%

Polyclonal Distribution of blaOXA-23 Gene Among Acinetobacter baumannii Isolated from Intensive Care Unit Patients in Tehran; Pulsed-Field Gel Electrophoresis Analysis

Dehbalaei

Peerayeh

Behmanesh

et al. 2017

Jundishapur J Microbiol

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Background: Despite a relatively low virulence of Acinetobacter baumannii isolates, emerging multidrug-resistant (MDR) strains to pose a formidable threat to patients, particularly in ill patients in intensive care unit (ICU). Objectives: The aim of the present study was to determine the genetic relatedness and antimicrobial susceptibility patterns in the endemic clones of A. baumannii isolated from patients in the ICU. Methods: Fifty-five non-repetitive A. baumannii isolates were examined for antimicrobial susceptibility, oxacillinase genes, class 1 integrons and genetic relationships by PFGE. Results: Antibiotic susceptibility testing showed that 21.8%) 12 isolates) were resistant to all tested antibiotics. Resistant to carbapenems were up to 85%. OXA-23, OXA-24 and OXA-58 genes were detected in 81.81%, 16.36% and 1.81% isolates respectively. The ISAba1 element upstream of blaOXA-51 was detected in 18 (32%) isolates, and 22 (40%) isolates had an ISAba1 insertion sequence upstream of the blaOXA-23. Integron class 1 was detected in 25 (55.5%) OXA-23 carrying isolates, 2 (22.2%) in OXA-24 positive isolates and in one OXA-58 carrying isolate. PFGE analysis resolved 50 distinct pulsotypes, Most of the isolates were scattered throughout across the dendrogram and a few grouped as clusters. Conclusions: No significant association has been found between the pulsotype of each isolate and MDR patterns and the presence of carbapenemase genes, however, highly resistant blaOXA-23 gene carrying endemic clones of A. baumannii disseminated in the ICU of two hospitals. Therefore, active surveillance and health policies are urgently needed for the detection and control the dissemination of such organisms.

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“…Among the several factors, the biofilm-associated protein encoded by the bap gene plays an important role in intercellular adhesion, accumulation of bacterial cells, and establishment of biofilm [7,8]. In the literature reports, the presence and expression of the bla PER-1 gene has been identified to encourage the clinical isolates of A. baumannii to form biofilm and adhere to respiratory epithelial cells [9,10,11].…”

Section: Introductionmentioning

confidence: 99%

Biofilm Formation in Acinetobacter Baumannii: Genotype-Phenotype Correlation

et al. 2019

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Strains of Acinetobacter baumannii are commensal and opportunistic pathogens that have emerged as problematic hospital pathogens due to its biofilm formation ability and multiple antibiotic resistances. The biofilm-associated pathogens usually exhibit dramatically decreased susceptibility to antibiotics. This study was aimed to investigate the correlation of biofilm-forming ability, antibiotic resistance and biofilm-related genes of 154 A. baumannii isolates which were collected from a teaching hospital in Taiwan. Biofilm-forming ability of the isolates was evaluated by crystal violet staining and observed by scanning electron microscopy. Antibiotic susceptibility was determined by disc diffusion method and minimum inhibitory concentration; the biofilm-related genes were screened by polymerase chain reaction. Results showed that among the 154 tested isolates, 15.6% of the clinical isolates were weak biofilm producers, while 32.5% and 45.4% of them possessed moderate and strong biofilm formation ability, respectively. The experimental results revealed that the multiple drug resistant isolates usually provided a higher biofilm formation. The prevalence of biofilm related genes including bap, blaPER-1, csuE and ompA among the isolated strains was 79.2%, 38.3%, 91.6%, and 68.8%, respectively. The results indicated that the antibiotic resistance, the formation of biofilm and the related genes were significantly correlated. The results of this study can effectively help to understand the antibiotic resistant mechanism and provides the valuable information to the screening, identification, diagnosis, treatment and control of clinical antibiotic-resistant pathogens.

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Clonal relatedness and biofilm formation of OXA-23-producing carbapenem resistant Acinetobacter baumannii isolates from hospital environment

Cited by 20 publications

References 25 publications

Genetic Diversity of OXA Producing Carbapenem-Resistant Acinetobacter baumannii from Environment of Tertiary Hospitals in Central Iran

Genetic Diversity of OXA Producing Carbapenem-Resistant Acinetobacter baumannii from Environment of Tertiary Hospitals in Central Iran

Polyclonal Distribution of blaOXA-23 Gene Among Acinetobacter baumannii Isolated from Intensive Care Unit Patients in Tehran; Pulsed-Field Gel Electrophoresis Analysis

Biofilm Formation in Acinetobacter Baumannii: Genotype-Phenotype Correlation

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