Clonal relationship among Vibrio parahaemolyticus isolated from Mediterranean mussels (Mytilus galloprovincialis) and grooved carpet shells (Ruditapes decussatus) harvested in Sardinia (Italy)

Lamon, Sonia; Bastardo, Asmine; Meloni, Domenico; Consolati, Simonetta Gianna; Fois, Federica; Porcheddu, Gabriella; Agus, Vanessa; Pes, Margherita; Cambula, Maria Giovanna; Mureddu, A; Romalde, Jesús L.

doi:10.1016/j.fm.2019.103258

Cited by 7 publications

(6 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Typhimurium is involved in most of human infection outbreaks and its recovering could lead to products that are unsuitable for guaranteeing the safety of Sardinian consumers. Recent studies reporting collateral surveys carried out in M. galloprovincialis and R. decussatus harvested in the same period from the same production areas, highlighted the presence of potentially pathogenic V. parahaemolyticus isolates ( tdh + or trh +) too (Lamon et al ., 2019a ; Lamon et al , 2019b ).…”

Section: Discussionmentioning

confidence: 99%

Enumeration of Escherichia coli and determination of Salmonella spp. and verotoxigenic Escherichia coli in shellfish (Mytilus galloprovincialis and Ruditapes decussatus) harvested in Sardinia, Italy

Lamon¹,

Piras²,

Meloni³

et al. 2020

Ital J Food Safety

Self Cite

View full text Add to dashboard Cite

The aim of the present study was to evaluate the occurrence of Salmonella spp., Verotoxigenic E. Coli (VTEC) and enumerate E. coli in shellfish (Mytilus galloprovincialis and Ruditapes decussatus) collected before and after depuration from two class B harvesting areas located in Sardinia (Italy). All the samples were analyzed for Salmonella spp. detection according to European Commission Regulation (EC) 2073/2005 and examined using the five tube Most-Probable-Number (MPN) method for enumeration of E. coli in accordance with the European Union reference method ISO 16649-3:2015. E. coli VTEC was investigated following a direct multiplex Polymerase Chain Reaction (PCR) screening test. The enumeration of E. coli met the European law limit for Class A areas of 230 MPN/100g. The averaged enumeration of E. coli in samples of M. galloprovincialis and R. decussatus collected at the harvesting time was 39 and 37 MPN/100 g respectively. The average contamination levels in samples collected after purification were 58 MPN/100g (M. galloprovincialis) and 32 MPN/100 g (R. decussatus). E. coli VTEC was not detected, on the contrary, Salmonella ser. Typhimurium was detected in one sample of M. galloprovincialis and in one sample of R. decussatus collected at the harvesting time. No significant associations were observed between E. coli levels in shellfish and environmental parameters of water or with the detection of Salmonella ser. Typhimurium in M. galloprovincialis and R. decussatus samples. Nevertheless, the occurrence of Salmonella ser. Typhimurium, involved in human infection outbreaks, should be considered a potential risk for consumers.

show abstract

Section: Discussionmentioning

confidence: 99%

Enumeration of Escherichia coli and determination of Salmonella spp. and verotoxigenic Escherichia coli in shellfish (Mytilus galloprovincialis and Ruditapes decussatus) harvested in Sardinia, Italy

Lamon¹,

Piras²,

Meloni³

et al. 2020

Ital J Food Safety

Self Cite

View full text Add to dashboard Cite

show abstract

“…The comparison of typing approaches enables the user to retain a convenient method while discarding an ineffective one (Abdel-Rhman and Rizk, 2021). As proved in the previous study, majority of these methods have limitations in terms of resolving evolutionary relationships, particularly when employed in isolation-no single method is sufficient to analyse the genetic relatedness of the endemic environmental V. cholerae population in an unambiguous manner (Lamon et al, 2019). Occasionally, single analyses may fail to give sufficient resolution or may produce inconsistent results.…”

Section: Resultsmentioning

confidence: 95%

“…Occasionally, single analyses may fail to give sufficient resolution or may produce inconsistent results. As a result, a combination of approaches may be necessary to completely type all V. cholerae strains, hence increasing phylogenetic accuracy and resolution (Lamon et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

Random amplified polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus (ERIC) PCR of Vibrio cholerae from a foodborne outbreak in Limbang, Sarawak

Elexson,

Ja'afar,

Joel

et al. 2023

IFRJ

View full text Add to dashboard Cite

Toxigenic and non-toxigenic V. cholerae strains can be monitored for changes in clones or serogroups, linkages between clinical and environmental isolates, genesis and clonal selection of epidemic strains, and population structure. Also, determining genetic relatedness among V. cholerae strains is critical for determining population genetic structure and evolutionary trends. In collaboration with the Sarawak Government Hospital, the present work was carried out on a total of 16 V. cholerae isolates in order to determine the genetic relatedness or heterogeneity of V. cholerae isolates from a foodborne outbreak among guests who attended a wedding ceremony in Limbang, Sarawak, Malaysia. The random amplified polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus (ERIC) were conducted to compare and determine strains and trace disease-causing microorganism. The RAPD fingerprinting was conducted using a total of 10-mer oligos 100 nmole random primers (OPAE1 - OPAE20). The primers OPAE7, OPAE10, OPAE14, and OPAE17 were selected because they were the most stable and discriminatory for V. cholerae. The PCR fingerprinting of ERIC-PCR was carried out using primer set of ERIC, ERIC1R (5’- ATGTAAGCTCCTGGGGATTCAC-3’), and ERIC2F (5’- AAGTAAGTGACTGGGGTGAGCG-3’). As a result, the 1 confirmed V. cholerae O1 samples were successfully fingerprinted. Based on the profiling results, the genetic fingerprint of some of the isolates from the clinical and environmental samples had 100% similarity, as indicated by the dendrogram. This indicated that the strains shared the same genetic profile. The smaller the genetic distance, the more homogeneous the strains are. The clinical and environmental strains shared some genetic characteristics. As indicated by the dendrogram, some strains were found to be closely linked to one another, while others were heterogeneous. Therefore, RAPD-PCR and ERIC-PCR produced the highest discrimination index. By combining these typing methods, evaluation of isolates' genetic diversity may be improved. The findings of the present work demonstrated the need for continued surveillance of V. cholera in Sarawak, Malaysia.

show abstract

“…Our results are substantially in accordance with other studies on viable V. parahaemolyticus in frozen products. In fact, viable Vibrio spp., including V. parahaemolyticus , are commonly isolated from fresh bivalve shellfish worldwide [ 100 , 101 , 102 ] but their occurrence in frozen products is less common. Panebianco et al [ 28 ], on 81 samples of frozen bivalve molluscs, isolated V. parahaemolyticus in 3.24% of samples (clams, scallops, mussels).…”

Section: Discussionmentioning

confidence: 99%

“…Similar results showing clams as the main source of V. parahaemolyticus were obtained by Lopatek et al [ 103 ] (31 of 120 clam samples, 25.8%) and Roque et al [ 104 ] (38 of 90 clam samples, 42.2%). Lamon et al [ 100 , 102 ] detected higher loads of Vibrio spp. in clams ( Ruditapes decussatus ) than in mussels ( Mytilus galloprovincialis ) collected in Sardinia (Italy).…”

Section: Discussionmentioning

confidence: 99%

Quantitative Detection of Viable but Nonculturable Vibrio parahaemolyticus in Frozen Bivalve Molluscs

Salvo

Panebianco

et al. 2023

Foods

View full text Add to dashboard Cite

Vibrio parahaemolyticus is a foodborne pathogen diffusely distributed in the marine environment and often isolated from raw seafood belonging to different species, mostly shellfish. Ingestion of under- or uncooked seafood contaminated by V. parahaemolyticus can cause severe gastrointestinal symptoms in humans. Due to its ability to withstand low temperatures, Vibrio spp. could survive in frozen seafoods for long periods by entering the viable but nonculturable state (VBNC) and may constitute an unrecognized source of food contamination and infection. In the present study, seventy-seven frozen bivalve molluscs (35 mussels; 42 clams) were subjected to the detection and enumeration of viable V. parahaemolyticus using standard culture methods. VBNC forms were detected and quantified by applying an optimized protocol based on Propidium Monoazide (PMA) and Quantitative PCR (qPCR). All samples were negative for both the detection and enumeration of V. parahaemolyticus by the standard culture methods. VBNC forms were detected in 11.7% of the samples (9/77), with values ranging from 1.67 to 2.29 Log CFU/g. Only clam samples were positive for the detection of VBNC forms. The results of this study highlighted that VBNC V. parahaemolyticus may be present in frozen bivalve molluscs. Further data on the prevalence of VBNC V. parahaemolyticus in frozen seafood are needed in order to perform a robust risk assessment.

show abstract

Clonal relationship among Vibrio parahaemolyticus isolated from Mediterranean mussels (Mytilus galloprovincialis) and grooved carpet shells (Ruditapes decussatus) harvested in Sardinia (Italy)

Cited by 7 publications

References 73 publications

Enumeration of <em>Escherichia coli</em> and determination of <em>Salmonella</em> spp. and verotoxigenic <em>Escherichia coli</em> in shellfish (<em>Mytilus galloprovincialis</em> and <em>Ruditapes decussatus</em>) harvested in Sardinia, Italy

Enumeration of <em>Escherichia coli</em> and determination of <em>Salmonella</em> spp. and verotoxigenic <em>Escherichia coli</em> in shellfish (<em>Mytilus galloprovincialis</em> and <em>Ruditapes decussatus</em>) harvested in Sardinia, Italy

Random amplified polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus (ERIC) PCR of Vibrio cholerae from a foodborne outbreak in Limbang, Sarawak

Quantitative Detection of Viable but Nonculturable Vibrio parahaemolyticus in Frozen Bivalve Molluscs

Contact Info

Product

Resources

About