Clonal Relationship and Resistance Profiles Among ESBL-Producing Escherichia coli

Abstract

AmpC β-lactamases hydrolyze all β-lactams except cefepime and carbapenems. The study of AmpC-producing E. coli has high priority for the infection control committee. This research is aimed to investigate the resistant urinary AmpC-generating E. coli isolates and identify their genetic variety. Some 230 E. coli isolates from patients suffering urinary tract infection symptoms were studied in 2017–2018 to assess their susceptibility toward antimicrobial agents. AmpC gene was evaluated by PCR and molecular typing using the 10-loci MLVA method. MLVA images were examined by BioNumerics 6.6 software through the use of the UPGMA algorithms. Thirty-eight AmpC-generating E. coli isolates were detected. The most abundant determinant was blaCIT and blaEBC, blaFOX, and blaDHA had the next ranks, respectively. Six major clusters and a singleton were identified by MLVA. AmpC beta-lactamases in urinary isolates of E. coli in the hospital under study and high rate of additional resistance to gentamicin, cotrimoxazole and ciprofloxacin. The most frequent gene determinant of AmpC beta-lactamase was blaCIT and vary depending on time and geographical location.

Distribution and expression of virulence genes (hlyA, sat) and genotyping of Escherichia coli O25b/ST131 by multi-locus variable number tandem repeat analysis in Tehran, Iran

“…However, the drawback of his method was the need for sequencing, which increased costs [41]. More loci can improve the differentiating power, so eight to ten loci have been used [39,42]. All strains had the above loci, while in the study of Caméléna et al, 23% of strains did not have one or two VNTR loci [23].…”

Amygdalin Reverses Macrophage PANoptosis Induced by Drug-Resistant Escherichia coli

“…It is also a member of the commensal flora in the human and other mammalian intestines [ 1 ]. As a result of antibiotic abuse, E. coli strains encoding extended-spectrum β-lactamases (ESBL) have emerged, raising concerns about the lack of effective treatment choices for E. coli infections and effectively negating the use of whole classes of antibiotics leaving few alternatives [ 2 ]. Infections caused by drug-resistant E. coil are a cause of concern for medical establishments due to the high morbidity and mortality rates associated with them [ 3 ].…”