Clonal selection of human immunodeficiency virus (HIV): Serological differences in the envelope antigens of the cloned viruses and HIV prototypes (HTLV-III B, LAV, and ARV)

Harada, Shinji; Kobayashi, Nobuyuki; Koyanagi, Yoshio; Yamamoto, Nobuto

doi:10.1016/0042-6822(87)90219-4

Cited by 28 publications

(8 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3). It was also found that the titers of neutralizing antibodies against both clones in the serum used in this RIP experiment were almost the same (10). Taken together, we can conclude that we are handling 2 clones producing almost the same amount of gp120 and other viral proteins, but having a marked difference in fusion activity ( Fig.…”

Section: Discussionsupporting

confidence: 65%

Syncytium‐Inducing Capacity of Human Immunodeficiency Virus (HIV): Analysis by the Use of Cloned Viruses

El‐Farrash

Harada

1989

Microbiology and Immunology

Self Cite

View full text Add to dashboard Cite

The marked cytopathic effects of human immunodeficiency virus (HIV) for susceptible cells are caused mainly by fusion between cells expressing viral envelope glycoproteins and cells expressing CD4 molecule. In this study, we tested the ability of different clones of HIV to induce syncytia in CD4-positive cells. We have reported marked difference in syncytium-inducing capacity of 2 clones of human T lymphotropic virus type III (HTLV-IIIB) isolate despite no detectable difference in expression of viral glycoprotein (gp120). This difference in syncytium induction could be explained by the difference detected in their infectivity and binding activities to CD4-positive cells. Meanwhile we reported difference in syncytium-inducing capacity of 2 clones of lymphadenopathy associated virus (LAVi) isolate parallel to the different amounts of gp 120 and other viral proteins expressed by these 2 clones. These results suggest that viral factors like infectivity and binding affinity of the virus to the susceptible cells and the amount of viral gp120 expressed by the infected cells may interact in a complex mannes affecting fusion activity and syncytium induction in CD4-positive cells.

show abstract

Section: Discussionsupporting

confidence: 65%

Syncytium‐Inducing Capacity of Human Immunodeficiency Virus (HIV): Analysis by the Use of Cloned Viruses

El‐Farrash

Harada

1989

Microbiology and Immunology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Thus, Harada et al (1987) showed HIV-1 SF2 to be 2-16 times more susceptible than HIV-1 (HTLV IIIB) and nine times more susceptible than HIV-1 LAV, Ho et al (1988) showed it to be 1-25 to two times more susceptible than HIV-1 (HTLV IIIB) and 1.39 to two times more susceptible than HIV-1 RF, and Steimer et al (1988) again showed it to be 10 times more susceptible than HIV-1 LAV. However, showed HIV-1 SF2 to have a level of neutralization similar to that of other isolates from the San Francisco area.…”

Section: Discussionmentioning

confidence: 99%

Antisera raised against the second variable region of the external envelope glycoprotein of human immunodeficiency virus type 1 cross-neutralize and show an increased neutralization index when they act together with antisera to the V3 neutralization epitope

Davis

Dm²,

Ca³

et al. 1993

Journal of General Virology

View full text Add to dashboard Cite

Antibodies have been raised against a synthetic peptide (IRDKIQKENALFRNL) containing a neutralizing epitope within the second variable region of the human immunodeficiency virus type 1 (HIV-1) SF2 strain external envelope glycoprotein (gpl20) and also against equivalent peptides of the HIV-1 LAI, RF and MN isolates. The resulting antisera cross-react with heterologous peptides but binding to heterologous recombinant gpl20 is more restricted. Antisera to HIV-1 SF2, RF and MN are able to neutralize homologous virus.Some cross-neutralization is also observed, but a consensus peptide failed to induce neutralizing antibodies to any of the isolates studied. Antibodies to the V2 and V3 epitopes give a higher neutralization index when acting together than when the individual sera are used alone. Antibodies induced in natural infection bind to two sets of hexamers within the region encompassed by the 15-mer peptide, and the response to these can differ between infected individuals and within the same host over time.

show abstract

“…HIV-1 used for in vitro infection was a filtered culture supernatant of MOLT4/LAI C-3 cells (11). For HIV-1 infection in vitro, CD4 + T-cells isolated from HIV-1 uninfected healthy donors were spinoculated with culture supernatants containing HIV-1 at a multiplicity of infection of 0.03 to 0.1 at room temperature for 1.5 h, extensively washed, and cultured in a medium containing recombinant human IL-2 at a concentration of 10 5 cells/200 lL in a 96-well round-bottom plate.…”

Section: In Vitro Hiv-1 Infection Of Cd4 + T-cellsmentioning

confidence: 99%

Allo-Antigen Stimulated CD8+ T-Cells Suppress NF-κB and Ets-1 DNA Binding Activity, and Inhibit Phosphorylated NF-κB p65 Nuclear Localization in CD4+ T-cells

et al. 2014

View full text Add to dashboard Cite

CD8 + T-cells of asymptomatic HIV-1 carriers (AC) suppress human immunodeficiency virus type 1 (HIV-1) replication in a class I major histocompatibility complex (MHC-I)-restricted and -unrestricted manner. In order to investigate the mechanism of MHC-I-unrestricted CD8 + T-cell-mediated HIV-1 suppression, we previously established allo-antigen stimulated CD8 + T-cells from HIV-1-uninfected donors. These allo-antigen stimulated CD8 + T-cells suppressed HIV-1 replication in acutely infected autologous CD4 + T-cells when directly cocultured. To elucidate the mechanism of HIV-1 replication suppression, we analyzed DNA-binding activity and phosphorylation of transcriptional factors associated with HIV-1 replication by electrophoresis mobility shift assay and Western blotting. When CD4 + T-cells were cultured with allo-antigen stimulated CD8 + T-cells, the reduction of NF-jB and Ets-1 DNA-binding activity was observed. Nuclear localization of NF-jB p65 and Ets-1 was suppressed in CD4 + T-cells. Although NF-jB p65 and Ets-1 are known to be regulated by protein kinase A (PKA), no difference was observed in the expression and phosphorylation of the PKA catalytic subunit in CD4 + T-cells cultured with PHA-treated CD8 + T-cells or allo-antigen stimulated CD8 + T-cells. Cyclic AMP is also known to enter through gap junctions, but the suppression of HIV-1 replication mediated by allo-antigen stimulated CD8 + T-cells was not affected by the gap junction inhibitor. The nuclear transport of phosphorylated NF-jB p65 (Ser276) was inhibited only in CD4 + T-cells cultured with allo-antigen stimulated CD8 + Tcells. Our results indicate that allo-antigen stimulated CD8 + T-cells suppress the transcriptional activity of NF-jB p65 or Ets-1 in an antigen-nonspecific manner, and inhibit the nuclear transport of phosphorylated NF-jB p65 (Ser276).

show abstract

Clonal selection of human immunodeficiency virus (HIV): Serological differences in the envelope antigens of the cloned viruses and HIV prototypes (HTLV-III B, LAV, and ARV)

Cited by 28 publications

References 19 publications

Syncytium‐Inducing Capacity of Human Immunodeficiency Virus (HIV): Analysis by the Use of Cloned Viruses

Syncytium‐Inducing Capacity of Human Immunodeficiency Virus (HIV): Analysis by the Use of Cloned Viruses

Antisera raised against the second variable region of the external envelope glycoprotein of human immunodeficiency virus type 1 cross-neutralize and show an increased neutralization index when they act together with antisera to the V3 neutralization epitope

Allo-Antigen Stimulated CD8+ T-Cells Suppress NF-κB and Ets-1 DNA Binding Activity, and Inhibit Phosphorylated NF-κB p65 Nuclear Localization in CD4+ T-cells

Contact Info

Product

Resources

About