Clonality of multidrug-resistant nontypeable strains of Haemophilus influenzae

Fusté, M. Carmen; Pineda, Marbin; Palomar, J; Viñas, Marc; Lorén, J. Gaspar

doi:10.1128/jcm.34.11.2760-2765.1996

Cited by 15 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The repeated findings on the strong genetic diversity of noncapsulated H. influenzae, which are consistent with the results described here using ERIC-PCR, allowed some authors to make hypothetical predictions suggesting the possibility of a nonclonal population for nontypeable H. influenzae (22). However, Fusté et al (9), who recently surveyed the extent of clonality within nontypeable strains, showed a basic clonal structure with little possibility of recombination. Mutations in the H. influenzae genome probably contribute to the strong variability of noncapsulated isolates, as observed using ERIC-PCR.…”

Section: Discussionsupporting

confidence: 88%

Genomic Variability of Haemophilus influenzae Isolated from Mexican Children Determined by Using Enterobacterial Repetitive Intergenic Consensus Sequences and PCR

et al. 2000

View full text Add to dashboard Cite

Genomic fingerprints from 92 capsulated and noncapsulated strains of Haemophilus influenzae from Mexican children with different diseases and healthy carriers were generated by PCR using the enterobacterial repetitive intergenic consensus (ERIC) sequences. A cluster analysis by the unweighted pair-group method with arithmetic averages based on the overall similarity as estimated from the characteristics of the genomic fingerprints, was conducted to group the strains. A total of 69 fingerprint patterns were detected in theH. influenzae strains. Isolates from patients with different diseases were represented by a variety of patterns, which clustered into two major groups. Of the 37 strains isolated from cases of meningitis, 24 shared patterns and were clustered into five groups within a similarity level of 1.0. One fragment of 1.25 kb was common to all meningitis strains. H. influenzae strains from healthy carriers presented fingerprint patterns different from those found in strains from sick children. Isolates from healthy individuals were more variable and were distributed differently from those from patients. The results show that ERIC-PCR provides a powerful tool for the determination of the distinctive pathogenicity potentials of H. influenzae strains and encourage its use for molecular epidemiology investigations.

show abstract

Section: Discussionsupporting

confidence: 88%

Genomic Variability of Haemophilus influenzae Isolated from Mexican Children Determined by Using Enterobacterial Repetitive Intergenic Consensus Sequences and PCR

et al. 2000

View full text Add to dashboard Cite

show abstract

“…Studies of the epidemiology of nontypeable strains prove the important heterogenieity of these strains (4,12,16,26). This heterogenieity is the same as that in AMP r ␤Ϫ H. influenzae strains, and Mendelman et al (23) have deduced that this resistance originated long ago.…”

Section: Discussionmentioning

confidence: 94%

Molecular Epidemiology of Ampicillin-Resistant Non-β-Lactamase-Producing Haemophilus influenzae

Gazagne

Delmas

Bingen³

et al. 1998

J Clin Microbiol

View full text Add to dashboard Cite

Resistance to ampicillin without β-lactamase production is not a frequent occurrence among Haemophilus influenzaestrains. This kind of resistance is encountered in unencapsulated strains isolated from bronchial secretions and ear, nose, and throat specimens and is exceptional in H. influenzae type b. We studied 29 of these strains from various areas in France and 2 reference strains. Strains were compared by using ribotyping, arbitarily primed PCR with two primers, and pulsed-field gel electrophoresis. Each technique enabled the identification of 20 to 23 different patterns among the 31 strains. The combination of the different patterns for the strains obtained by the different techniques provided 27 distinct profiles. According to these results, it seems that the clonal propagation of these resistant strains does not occur.

show abstract

“…To determine whether I A is significantly greater than zero, a Monte Carlo procedure can be performed. Computer programs written by J. G. Lorén (17) were used to calculate locus and genotypic diversity, to calculate I A , and to perform the Monte Carlo simulations. Figure 1 illustrates the RFLP and PFGE patterns obtained with nine isolates from the mother, the father, and two children of family A.…”

Section: Methodsmentioning

confidence: 99%

Helicobacter pylori : Clonal Population Structure and Restricted Transmission within Families Revealed by Molecular Typing

Han¹,

Zschausch²,

Meyer³

et al. 2000

J Clin Microbiol

View full text Add to dashboard Cite

Helicobacter pylori infects up to 50% of the human population worldwide. The infection occurs predominantly in childhood and persists for decades or a lifetime. H. pylori is believed to be transmitted from person to person. However, tremendous genetic diversity has been reported for these bacteria. In order to gain insight into the epidemiological basis of this phenomenon, we performed molecular typing of H. pylori isolates from different families. Fifty-nine H. pylori isolates from 27 members of nine families were characterized by using restriction fragment length polymorphism analysis of five PCR-amplified genes, by pulsed-field gel electrophoresis (PFGE) of chromosomal DNA, and byvacA and cagA genotyping. The 16S rRNA gene exhibited little allelic variation, as expected for a unique bacterial species. In contrast, the vacA, flaA,ureAB, and lspA-glmM genes were highly polymorphic, with a mean genetic diversity of 0.83, which exceeds the levels recorded for all other bacterial species. In conjunction with PFGE, 59 H. pylori isolates could be differentiated into 21 clonal types. Each individual harbored only one clone, occasionally with a clonal variant. Identical strains were always found either between siblings or between a mother and her children. Statistical analysis revealed clonality of population structure in all isolates. The results of this study suggest the possible coexistence of a large array of clonal lineages that are evolving in each individual in isolation from one another. Transmission appears to occur primarily from mother to child and perhaps between siblings.

show abstract

Clonality of multidrug-resistant nontypeable strains of Haemophilus influenzae

Cited by 15 publications

References 19 publications

Genomic Variability of Haemophilus influenzae Isolated from Mexican Children Determined by Using Enterobacterial Repetitive Intergenic Consensus Sequences and PCR

Genomic Variability of Haemophilus influenzae Isolated from Mexican Children Determined by Using Enterobacterial Repetitive Intergenic Consensus Sequences and PCR

Molecular Epidemiology of Ampicillin-Resistant Non-β-Lactamase-Producing Haemophilus influenzae

Helicobacter pylori : Clonal Population Structure and Restricted Transmission within Families Revealed by Molecular Typing

Contact Info

Product

Resources

About