Cloneless genomic DNA analysis: an efficient and simple methods for de novo genomic sequencing projects and gap filling

Nguyen, Giang Huong; Bukanov, Nikolayev; Oshimura, Mitsuo; Smith, Cassandra L.

doi:10.1016/j.bioeng.2004.08.002

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Comparative Genomics Approaches and Technologies

Smith

Stamenović

2012

Encyclopedia of Analytical Chemistry

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Comparative molecular approaches used DNA sequencing and less costly alternatives. Today new DNA sequencing methods are dramatically impacting comparative genomics studies and many old approaches are being adapted or enhanced by the ultra‐high‐throughput sequencing (UHTPS) methods. Even so, the expenses of the newer methods limits their access, while the older methods continue to be improved and applied widely. This article reviews comparative genomics approaches used today but does not review UHTPS methods. A subsequent article by us (Smith and Oh, in preparation) will focus on the impact of the new UHTPS methods on comparative genomics.

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