2024
DOI: 10.3390/genes15030315
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Cloning and Analysis of Expression of Genes Related to Carotenoid Metabolism in Different Fruit Color Mutants of Pepper (Capsicum annuum L.)

Penglong Feng,
Yayi Wang,
Junqin Wen
et al.

Abstract: The formation of fruit color in pepper is closely related to the processes of carotenoid metabolism. In this study, red wild-type pepper XHB, SP01, PC01 and their corresponding mutants H0809 (orange), SP02 (yellow), and PC02 (orange) were used as research materials. The Ggps, Psy, Lcyb, Crtz, Zep, and Ccs genes involved in carotenoid biosynthesis were cloned, and bioinformatics and expression analyses were carried out. The results showed that the full lengths of the six genes were 1110 bp, 2844 bp, 1497 bp, 20… Show more

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“…Several studies have undertaken research on the phenotypic manifestations, quantitative compositions, and chromosomal allocations of carotenoids and flavonoids within botanical specimens [6][7][8][9][10]. Feng [11] used three wildtype red peppers and their corresponding mutants (yellow and orange) as research materials to clone the genes involved in carotenoid biosynthesis, including GGPS, PSY, LCYB, CRTZ, ZEP, and CCS. It was discovered that in two mutant types, the complete CCS gene could not be cloned.…”
Section: Introductionmentioning
confidence: 99%
“…Several studies have undertaken research on the phenotypic manifestations, quantitative compositions, and chromosomal allocations of carotenoids and flavonoids within botanical specimens [6][7][8][9][10]. Feng [11] used three wildtype red peppers and their corresponding mutants (yellow and orange) as research materials to clone the genes involved in carotenoid biosynthesis, including GGPS, PSY, LCYB, CRTZ, ZEP, and CCS. It was discovered that in two mutant types, the complete CCS gene could not be cloned.…”
Section: Introductionmentioning
confidence: 99%