Cloning and analysis of gene expression of interleukin-17 homolog in triangle-shell pearl mussel, Hyriopsis cumingii, during pearl sac formation

Zhang, Rui; Wang, Meng; Ni, Xiaomin; Yu, Shuang; Chen, Yi; Wang, Ning

doi:10.1016/j.fsi.2016.03.027

Cited by 13 publications

(8 citation statements)

References 34 publications

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“…For example, in Pacific oyster, IL17 mRNA was detected in all examined tissues, including hemocytes, heart, gills, digestive glands, gonad, mantle and muscle, and highly expressed in the gills and digestive glands tissues [ 23 ]. For freshwater pearl mussel Hyriopsis cumingii , IL17 was also found in any tissues tested, with the gills and digestive glands having the highest levels of IL17 [ 29 ]. In the present study, Mc IL17-1 also showed a broad spectrum of expression and significantly higher expressed in gills and hemocytes compared with that in other tissues.…”

Section: Discussionmentioning

confidence: 99%

“…In Pinctada fucata , IL17 has proven to be capable of activating the NF-κB signal pathway against exogenous pathogens [ 25 ]. Several IL17s and -17Rs have also been identified in other mollusks, including Haliotis discus hannai and Mytilus galloprovincialis [ 26 , 27 , 28 , 29 ], which provided valuable clues to elucidate the functional roles of IL17s in innate immune regulation in mollusks.…”

Section: Introductionmentioning

confidence: 99%

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An Interleukin-17 Isoform from Thick Shell Mussel Mytilus coruscus Serves as a Mediator of Inflammatory Response

et al. 2023

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The inflammatory cytokine interleukin-17 (IL17) plays an important role in innate immunity by binding to its receptors (IL17Rs) to activate immune defense signals. To date, information on members of the IL17 family is still very limited in molluscan species. Here, a novel member of the IL17 family was identified and characterized from thick shell mussel Mytilus coruscus, and this gene was designated as McIL17-1 by predicting structural domains and phylogenetic analysis. McIL17-1 transcripts existed in all examined tissues with high expression levels in gills, hemocytes and digestive glands. After the stimuli of different pathogen associated molecular patterns (PAMPs) for 72 h, transcriptional expression of McIL17-1 was significantly upregulated, except for poly I:C stimulation. Cytoplasm localization of McIL17-1 was shown in HEK293T cells by fluorescence microscopy. Further, in vivo and in vitro assays were performed to evaluate the potential function of McIL17-1 played in immune response. McIL17-1 was either knocked down or overexpressed in vivo through RNA inference (RNAi) and recombinant protein injection, respectively. With the infection of living Vibrio alginolyticus, a high mortality rate was exhibited in the McIL17-1 overexpressed group compared to the control group, while a lower mortality rate was observed in the McIL17-1 knocked down group than control group. In vitro, the flow cytometric analysis showed that the apoptosis rate of McIL17-1 inhibited hemocytes was significantly lower than that of the control group after lipopolysaccharide stimulation. These results collectively suggested that the newly identified IL17 isoform is involved in the inflammatory response to bacterial infection in M. coruscus.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An Interleukin-17 Isoform from Thick Shell Mussel Mytilus coruscus Serves as a Mediator of Inflammatory Response

et al. 2023

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“…The collected mussels were cultured for 2 weeks in our laboratory before any experiments. The laboratory culture of the mussels and mantle tissue transplantation surgery were performed as described in our previous report (Zhang et al 2016). Hyriopsis cumingii without mantle tissue transplantation were killed and their tissue anatomy was analyzed.…”

Section: Experimental Animals and Tissue Samplingmentioning

confidence: 99%

“…Real-time quantitative PCR components were mixed in a total reaction volume of 20:10 μl of 2 × SYBR ExTaqII premix (TaKaRa, Dalian, China), the forward and reverse primers (10 μM) and 1 μl diluted template cDNA (about 10 ng). The PCR condition was described previously (Zhang et al 2016;Mao et al 2017): pre-incubation at 95 °C for 30 s, followed by 40 cycles of denaturation at 95 °C for 5 s and annealing and extension at 60 °C for 34 s. Dissociation curve analysis (60-95 °C) was implemented to confirm the absence of nonspecific products. The primers used for this study were listed in Table 1.…”

Section: Real-time Quantitative Pcr Analysismentioning

confidence: 99%

Molecular cloning and characterization of Pif gene from pearl mussel, Hyriopsis cumingii, and the gene expression analysis during pearl formation

et al. 2018

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In the present study, the Pif gene of the freshwater pearl aquaculture mussel, (HcPif) was successfully cloned and functionally characterized. The full sequence of HcPif gene consists of 3415 base pairs, which putatively encode two proteins, HcPif90 and HcPif80. A sequence analysis revealed that HcPif contained a von Willebrand factor type A domain and a chitin-binding domain, and shared many functional residues with other Pif homologues. A highly conserved sequence, FKGLDEIELML, at the C-terminus of Pif80s was identified as the key functional site. The corresponding peptide fragment markedly modified the morphology of calcite crystallites in CaCO crystallization assay and might play an essential role in the interactive binding between HcPif80 and CaCO. Moreover, real-time PCR results showed that HcPif gene was dominantly expressed in the pearl secreting tissues and its expression changed in response to the different development status of the pearl sac during pearl aquaculture. The gene expression of HcPif was maximum 7 days after mantle grafting and declined to about the control level on day 30. Our in vitro and in vivo experimental data indicated that HcPif gene possessed the inherent characteristics of a nacre formation gene and its expression might faithfully reflect the pearl secretion status of the pearl mussels examined. Our findings may extend the understanding of the biomineralization mechanism of nacre formation and provide a potential biomarker for pearl farming.

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“…For example, transcriptomic sequencing has been used to evaluate immune system response mechanisms after xenotransplantation and allotransplantation (Wei et al, 2017). These methods have also been applied to investigations of freshwater pearl oysters (Zhang et al, 2016). RNA-seq based transcriptomic analysis is a highly effective approach for investigating the immune responses at the genomic and transcriptomic levels.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis reveals immune-related genes involved in allograft and xenograft transplantation in Pinctada fucata

Zheng,

Wang,

Guo

et al. 2024

Front. Mar. Sci.

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BackgroundThe marine pearl culture industry is a key industry in the Beibu Gulf of China that achieves large-scale pearl production by artificial nucleus insertion in pearls. High-quality pearls can produced by xenotransplantation, but allotransplantation or xenotransplantation can lead to various immune responses, resulting in nucleus rejection or even the recipient shell death and thereby causing significant losses in pearl production.MethodsFew studies have investigated the immune defenses of oysters related to allografts and xenografts. In this study, transcriptomic comparisons of allograft and xenograft Pinctada fucata haemocytes were conducted to identify genes associated with immune responses.ResultsA total of 33.11 Gbp of clean reads were generated from five P. fucata haemocytes. De-novo assembly of quality-filtered reads generated a total of 26,526 unigenes, with 22,002 known genes and 4,524 predicted novel genes. In addition, 34,904 novel transcripts were detected, with 15,620 novel alternative splicing isoforms of known protein coding genes and 4,605 belonging to novel protein coding genes, with the remaining 14,679 comprising long non-coding RNA transcripts. Functional enrichment analysis of immune-related differentially expressed genes (DEGs) using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases revealed 36–44 significantly enriched GO terms and 34 significantly enriched KEGG pathways. Ten DEGs were subjected to validation of expression levels using RT-q PCR analysis, revealing generally consistent values as the high-throughput sequencing data.ConclusionOyster haemocytes were comprehensively evaluated in this study using transcriptomic comparisons and with a focus on immune-related functional genes and pathways. The results revealed numerous DEGs related to immune function that can serve as the basis for subsequent immune response analysis of allotransplantation and xenotransplantation.

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Cloning and analysis of gene expression of interleukin-17 homolog in triangle-shell pearl mussel, Hyriopsis cumingii, during pearl sac formation

Cited by 13 publications

References 34 publications

An Interleukin-17 Isoform from Thick Shell Mussel Mytilus coruscus Serves as a Mediator of Inflammatory Response

An Interleukin-17 Isoform from Thick Shell Mussel Mytilus coruscus Serves as a Mediator of Inflammatory Response

Molecular cloning and characterization of Pif gene from pearl mussel, Hyriopsis cumingii, and the gene expression analysis during pearl formation

Comparative transcriptome analysis reveals immune-related genes involved in allograft and xenograft transplantation in Pinctada fucata

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