1997
DOI: 10.1128/jb.179.15.4821-4830.1997
|View full text |Cite
|
Sign up to set email alerts
|

Cloning and analysis of the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis genes of Aeromonas caviae

Abstract: A 5.0-kbp EcoRV-EcoRI restriction fragment was cloned and analyzed from genomic DNA of Aeromonas caviae, a bacterium producing a copolyester of (R)-3-hydroxybutyrate (3HB) and (R)-3-hydroxyhexanoate (3HHx) [P(3HB-co-3HHx)] from alkanoic acids or oils. The nucleotide sequence of this region showed a 1,782-bp poly (3-hydroxyalkanoate) (PHA) synthase gene (phaC Ac [i.e., the phaC gene from A. caviae]) together with four open reading frames (ORF1, -3, -4, and -5) and one putative promoter region. The cloned fragme… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

3
260
2
2

Year Published

2000
2000
2012
2012

Publication Types

Select...
8
2

Relationship

0
10

Authors

Journals

citations
Cited by 255 publications
(267 citation statements)
references
References 49 publications
3
260
2
2
Order By: Relevance
“…Previously, Kolibachuk and co-workers noticed that 3HHx monomer was accumulated by recombinant C. necator PHBˉ4 harboring the PHA synthase gene of C. violaceum when fed with even carbon numbered fatty acids (Kolibachuk et al, 1999). Similar studies were also done to generate 3HHx monomer by expressing PHA synthase gene of A. caviae in mutant C. necator PHB -4 (Fukui and Doi, 1997). A. caviae is known to synthesize both SCL and MCL PHA containing four to six carbon atoms (Shimamura et al, 1994).…”
Section: Carbon Sourcesmentioning
confidence: 84%
“…Previously, Kolibachuk and co-workers noticed that 3HHx monomer was accumulated by recombinant C. necator PHBˉ4 harboring the PHA synthase gene of C. violaceum when fed with even carbon numbered fatty acids (Kolibachuk et al, 1999). Similar studies were also done to generate 3HHx monomer by expressing PHA synthase gene of A. caviae in mutant C. necator PHB -4 (Fukui and Doi, 1997). A. caviae is known to synthesize both SCL and MCL PHA containing four to six carbon atoms (Shimamura et al, 1994).…”
Section: Carbon Sourcesmentioning
confidence: 84%
“…Similar genes were found in other bacteria that have in common with R. sphaeroides the absence of a functional glyoxylate cycle for acetate assimilation. However, the overall sequence identity of these proteins to the characterized member of this so-called MaoC or FkbR2 family, an R-specific 3-hydroxyacyl-CoA dehydratase from Aeromonas caviae (18), is only in the range of 20%. Furthermore, the expected molecular mass of the encoded enzyme is about 38 kDa, which is larger than the masses of normal enoyl-CoA hydratases (14 to 28 kDa).…”
Section: Resultsmentioning
confidence: 99%
“…One is PHA synthase of Thiocapsa pfennigii, which has two subunits, PhaC and PhaE, with approximately 85% similarity, respectively, to those of A. vinosum (class III), but unlike A. vinosum it can incorporate medium-as well as short-chain-length HACoAs into the polymer (11). The other exception is PHA synthase of Aeromonas caviae, which has approximately 45% similarity to that of R. eutropha (class I) and can incorporate 3-hydroxyhexanoyl thioester monomers into the polymer (5).…”
Section: Alcaligenes Eutrophus)mentioning
confidence: 99%