Abstract:The main pigment which affects flower colors is the anthocyanin, and dihydroflavonol 4-reductase (DFR) is a key enzyme in anthocyanin synthesis. In this study, we employed the native pigmented potato varieties 'Jianchuanhong' and 'Zhuanxinwu' from Yunnan Province, and the introduced potato variety 'Heimeiren' as the experimental materials. The complete cDNAs sequence of DFR was cloned from their tubers epidermis by RT-PCR, and the bioinformatics analysis was carried out. The sequence analysis displayed that DF… Show more
“…5 , most of the structural genes expressed in the tubers that were transcriptome sequenced showed a decreasing trend; however, structural genes that were expressed at the end of the synthetic pathway, such as 3GT (Flavonoids 3-O-glycosyltransferase), MT (Methyltransferase), and GST (Glutathione S-transferase), showed an increasing trend. The reason for this result may be because the amount of anthocyanin accumulation in the tubers sent for sequencing peaked, and most of the structural genes promoted the metabolism of phenylpropanoids and provided essential compounds for the metabolism of flavonoids in the latter stage [ 35 , 36 ], However, a significant increase in the expression of structural genes with end modified anthocyanins can prove that more anthocyanins are stably present in plants [ 37 ], indicating that low temperature can promote the progression of anthocyanin synthesis and the generation of more stable anthocyanins. Fig.…”
MYB transcription factors play an extremely important regulatory role in plant responses to stress and anthocyanin synthesis. Cloning of potato StMYB-related genes can provide a theoretical basis for the genetic improvement of pigmented potatoes. In this study, two MYB transcription factors, StMYB113 and StMYB308, possibly related to anthocyanin synthesis, were screened under low-temperature conditions based on the low-temperature-responsive potato StMYB genes family analysis obtained by transcriptome sequencing. By analyzed the protein properties and promoters of StMYB113 and StMYB308 and their relative expression levels at different low-temperature treatment periods, it is speculated that StMYB113 and StMYB308 can be expressed in response to low temperature and can promote anthocyanin synthesis. The overexpression vectors of StMYB113 and StMYB308 were constructed for transient transformation tobacco. Color changes were observed, and the expression levels of the structural genes of tobacco anthocyanin synthesis were determined. The results showed that StMYB113 lacking the complete MYB domain could not promote the accumulation of tobacco anthocyanins, while StMYB308 could significantly promote the accumulation involved in tobacco anthocyanins. This study provides a theoretical reference for further study of the mechanism of StMYB113 and StMYB308 transcription factors in potato anthocyanin synthesis.
“…5 , most of the structural genes expressed in the tubers that were transcriptome sequenced showed a decreasing trend; however, structural genes that were expressed at the end of the synthetic pathway, such as 3GT (Flavonoids 3-O-glycosyltransferase), MT (Methyltransferase), and GST (Glutathione S-transferase), showed an increasing trend. The reason for this result may be because the amount of anthocyanin accumulation in the tubers sent for sequencing peaked, and most of the structural genes promoted the metabolism of phenylpropanoids and provided essential compounds for the metabolism of flavonoids in the latter stage [ 35 , 36 ], However, a significant increase in the expression of structural genes with end modified anthocyanins can prove that more anthocyanins are stably present in plants [ 37 ], indicating that low temperature can promote the progression of anthocyanin synthesis and the generation of more stable anthocyanins. Fig.…”
MYB transcription factors play an extremely important regulatory role in plant responses to stress and anthocyanin synthesis. Cloning of potato StMYB-related genes can provide a theoretical basis for the genetic improvement of pigmented potatoes. In this study, two MYB transcription factors, StMYB113 and StMYB308, possibly related to anthocyanin synthesis, were screened under low-temperature conditions based on the low-temperature-responsive potato StMYB genes family analysis obtained by transcriptome sequencing. By analyzed the protein properties and promoters of StMYB113 and StMYB308 and their relative expression levels at different low-temperature treatment periods, it is speculated that StMYB113 and StMYB308 can be expressed in response to low temperature and can promote anthocyanin synthesis. The overexpression vectors of StMYB113 and StMYB308 were constructed for transient transformation tobacco. Color changes were observed, and the expression levels of the structural genes of tobacco anthocyanin synthesis were determined. The results showed that StMYB113 lacking the complete MYB domain could not promote the accumulation of tobacco anthocyanins, while StMYB308 could significantly promote the accumulation involved in tobacco anthocyanins. This study provides a theoretical reference for further study of the mechanism of StMYB113 and StMYB308 transcription factors in potato anthocyanin synthesis.
“…We analyzed the DFR gene and MYB-FL transcription factor regarding visible colors and UV-light response phenotypes. DFR is part of the anthocyanin biosynthesis pathway that occurs as a single-copy gene in several angiosperm species, such as Petunia [ 41 ] and Solanum [ 42 , 43 ], or duplicated, due to polyploidization as in Nicotiana [ 11 ].…”
Floral syndromes are known by the conserved morphological traits in flowers associated with pollinator attraction, such as corolla shape and color, aroma emission and composition, and rewards, especially the nectar volume and sugar concentration. Here, we employed a phylogenetic approach to investigate sequences of genes enrolled in the biosynthetic pathways responsible for some phenotypes that are attractive to pollinators in Solanaceae genomes. We included genes involved in visible color, UV-light response, scent emission, and nectar production to test the hypothesis that these essential genes have evolved by convergence under pollinator selection. Our results refuted this hypothesis as all four studied genes recovered the species’ phylogenetic relationships, even though some sites were positively selected. We found differences in protein motifs among genera in Solanaceae that were not necessarily associated with the same floral syndrome. Although it has had a crucial role in plant diversification, the plant–pollinator interaction is complex and still needs further investigation, with genes evolving not only under the influence of pollinators, but by the sum of several evolutionary forces along the speciation process in Solanaceae.
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